Experiment: protonated control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yadE and panD are separated by 3 nucleotides panD and yadD are separated by 273 nucleotides yadD and panC are separated by 73 nucleotides panC and panB are separated by 11 nucleotides
b0130: yadE - predicted polysaccharide deacetylase lipoprotein (NCBI), at 145,081 to 146,310
yadE
b0131: panD - aspartate 1-decarboxylase precursor (NCBI), at 146,314 to 146,694
panD
b0132: yadD - predicted transposase (NCBI), at 146,968 to 147,870
yadD
b0133: panC - pantoate--beta-alanine ligase (NCBI), at 147,944 to 148,795
panC
b0134: panB - 3-methyl-2-oxobutanoate hydroxymethyltransferase (NCBI), at 148,807 to 149,601
panB
Position (kb)
146
147
148 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 146.033 kb on + strand, within yadE at 146.034 kb on - strand, within yadE at 146.083 kb on + strand, within yadE at 146.142 kb on + strand, within yadE at 146.142 kb on + strand, within yadE at 146.153 kb on - strand, within yadE at 146.153 kb on - strand, within yadE at 146.275 kb on + strand at 146.275 kb on + strand at 146.277 kb on + strand at 146.277 kb on + strand at 146.461 kb on + strand, within panD at 146.513 kb on - strand, within panD at 146.602 kb on + strand, within panD at 146.627 kb on + strand, within panD at 146.632 kb on + strand, within panD at 146.791 kb on - strand at 146.901 kb on - strand at 146.901 kb on - strand at 146.917 kb on - strand at 146.964 kb on + strand at 146.979 kb on + strand at 146.979 kb on + strand at 146.982 kb on - strand at 146.985 kb on - strand at 147.127 kb on - strand, within yadD at 147.127 kb on - strand, within yadD at 147.127 kb on - strand, within yadD at 147.139 kb on - strand, within yadD at 147.154 kb on - strand, within yadD at 147.154 kb on - strand, within yadD at 147.164 kb on - strand, within yadD at 147.175 kb on - strand, within yadD at 147.268 kb on + strand, within yadD at 147.268 kb on + strand, within yadD at 147.278 kb on + strand, within yadD at 147.417 kb on - strand, within yadD at 147.436 kb on + strand, within yadD at 147.451 kb on - strand, within yadD at 147.466 kb on + strand, within yadD at 147.524 kb on + strand, within yadD at 147.524 kb on + strand, within yadD at 147.544 kb on - strand, within yadD at 147.558 kb on + strand, within yadD at 147.576 kb on - strand, within yadD at 147.631 kb on - strand, within yadD at 147.631 kb on - strand, within yadD at 147.663 kb on - strand, within yadD at 147.663 kb on - strand, within yadD at 147.665 kb on - strand, within yadD at 147.687 kb on - strand, within yadD at 147.696 kb on - strand, within yadD at 147.776 kb on + strand, within yadD at 147.806 kb on - strand at 147.806 kb on - strand at 147.820 kb on - strand at 147.820 kb on - strand at 147.901 kb on - strand at 147.954 kb on - strand at 147.984 kb on - strand at 147.984 kb on - strand at 147.999 kb on - strand at 148.026 kb on + strand at 148.042 kb on - strand, within panC at 148.042 kb on - strand, within panC at 148.063 kb on + strand, within panC at 148.063 kb on + strand, within panC at 148.202 kb on + strand, within panC at 148.205 kb on + strand, within panC at 148.205 kb on + strand, within panC at 148.215 kb on - strand, within panC at 148.215 kb on - strand, within panC at 148.236 kb on + strand, within panC at 148.259 kb on - strand, within panC at 148.376 kb on - strand, within panC at 148.376 kb on - strand, within panC at 148.385 kb on - strand, within panC at 148.463 kb on + strand, within panC at 148.484 kb on - strand, within panC at 148.488 kb on + strand, within panC at 148.547 kb on + strand, within panC at 148.570 kb on - strand, within panC at 148.570 kb on - strand, within panC at 148.573 kb on - strand, within panC at 148.624 kb on - strand, within panC at 148.624 kb on - strand, within panC at 148.643 kb on + strand, within panC at 148.787 kb on + strand at 148.787 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction protonated control remove 146,033 + yadE b0130 0.77 -0.0 146,034 - yadE b0130 0.77 +1.5 146,083 + yadE b0130 0.81 -0.1 146,142 + yadE b0130 0.86 -0.5 146,142 + yadE b0130 0.86 +0.7 146,153 - yadE b0130 0.87 -0.3 146,153 - yadE b0130 0.87 +0.8 146,275 + +0.4 146,275 + +0.4 146,277 + +0.7 146,277 + -0.1 146,461 + panD b0131 0.39 -0.3 146,513 - panD b0131 0.52 -2.6 146,602 + panD b0131 0.76 +0.5 146,627 + panD b0131 0.82 -1.1 146,632 + panD b0131 0.83 -1.5 146,791 - +1.1 146,901 - +0.1 146,901 - +0.8 146,917 - +0.5 146,964 + -0.7 146,979 + +0.4 146,979 + -0.2 146,982 - -0.4 146,985 - +0.0 147,127 - yadD b0132 0.18 +0.7 147,127 - yadD b0132 0.18 -0.3 147,127 - yadD b0132 0.18 +0.3 147,139 - yadD b0132 0.19 +0.4 147,154 - yadD b0132 0.21 +0.6 147,154 - yadD b0132 0.21 -0.4 147,164 - yadD b0132 0.22 +0.0 147,175 - yadD b0132 0.23 +0.7 147,268 + yadD b0132 0.33 +0.2 147,268 + yadD b0132 0.33 +0.2 147,278 + yadD b0132 0.34 +0.2 147,417 - yadD b0132 0.50 +0.6 147,436 + yadD b0132 0.52 -0.4 147,451 - yadD b0132 0.53 +0.9 147,466 + yadD b0132 0.55 -0.7 147,524 + yadD b0132 0.62 -1.0 147,524 + yadD b0132 0.62 -0.8 147,544 - yadD b0132 0.64 +1.5 147,558 + yadD b0132 0.65 +0.7 147,576 - yadD b0132 0.67 +0.6 147,631 - yadD b0132 0.73 -0.7 147,631 - yadD b0132 0.73 +0.7 147,663 - yadD b0132 0.77 -0.6 147,663 - yadD b0132 0.77 -0.5 147,665 - yadD b0132 0.77 +0.1 147,687 - yadD b0132 0.80 -0.0 147,696 - yadD b0132 0.81 +0.9 147,776 + yadD b0132 0.89 -1.0 147,806 - +0.3 147,806 - -1.3 147,820 - +1.2 147,820 - +1.6 147,901 - +0.8 147,954 - +0.2 147,984 - -0.9 147,984 - -1.4 147,999 - -0.5 148,026 + +0.5 148,042 - panC b0133 0.12 -2.2 148,042 - panC b0133 0.12 -2.9 148,063 + panC b0133 0.14 -2.0 148,063 + panC b0133 0.14 -0.0 148,202 + panC b0133 0.30 -1.5 148,205 + panC b0133 0.31 -1.0 148,205 + panC b0133 0.31 -0.6 148,215 - panC b0133 0.32 -1.5 148,215 - panC b0133 0.32 +1.7 148,236 + panC b0133 0.34 -1.5 148,259 - panC b0133 0.37 -1.0 148,376 - panC b0133 0.51 +0.1 148,376 - panC b0133 0.51 -1.3 148,385 - panC b0133 0.52 -1.1 148,463 + panC b0133 0.61 -2.0 148,484 - panC b0133 0.63 -1.7 148,488 + panC b0133 0.64 -0.9 148,547 + panC b0133 0.71 -1.1 148,570 - panC b0133 0.73 -1.2 148,570 - panC b0133 0.73 +0.7 148,573 - panC b0133 0.74 -2.2 148,624 - panC b0133 0.80 -0.4 148,624 - panC b0133 0.80 -0.2 148,643 + panC b0133 0.82 +1.1 148,787 + -1.2 148,787 + -1.7
Or see this region's nucleotide sequence