Experiment: NAG (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00345 and PS417_00350 are separated by 161 nucleotides PS417_00350 and PS417_00355 are separated by 51 nucleotides PS417_00355 and PS417_00360 overlap by 1 nucleotides
PS417_00345: PS417_00345 - copper-binding protein, at 66,901 to 67,536
_00345
PS417_00350: PS417_00350 - methionine ABC transporter substrate-binding protein, at 67,698 to 68,468
_00350
PS417_00355: PS417_00355 - metal ABC transporter permease, at 68,520 to 69,194
_00355
PS417_00360: PS417_00360 - methionine ABC transporter ATP-binding protein, at 69,194 to 70,201
_00360
Position (kb) 68
69
70 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 67.530 kb on + strand at 67.530 kb on + strand at 67.530 kb on + strand at 67.531 kb on - strand at 67.531 kb on - strand at 67.535 kb on - strand at 67.727 kb on - strand at 67.834 kb on + strand, within PS417_00350 at 67.835 kb on - strand, within PS417_00350 at 68.146 kb on + strand, within PS417_00350 at 68.146 kb on + strand, within PS417_00350 at 68.286 kb on + strand, within PS417_00350 at 68.286 kb on + strand, within PS417_00350 at 68.695 kb on + strand, within PS417_00355 at 68.696 kb on - strand, within PS417_00355 at 68.696 kb on - strand, within PS417_00355 at 68.709 kb on - strand, within PS417_00355 at 69.090 kb on + strand, within PS417_00355 at 69.196 kb on - strand at 70.178 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction NAG (C) remove 67,530 + +1.1 67,530 + +0.4 67,530 + -0.5 67,531 - -0.5 67,531 - -0.2 67,535 - +0.1 67,727 - -0.3 67,834 + PS417_00350 0.18 +0.1 67,835 - PS417_00350 0.18 +0.4 68,146 + PS417_00350 0.58 -0.5 68,146 + PS417_00350 0.58 +0.0 68,286 + PS417_00350 0.76 -0.2 68,286 + PS417_00350 0.76 -0.9 68,695 + PS417_00355 0.26 -2.9 68,696 - PS417_00355 0.26 -2.4 68,696 - PS417_00355 0.26 -1.1 68,709 - PS417_00355 0.28 -0.3 69,090 + PS417_00355 0.84 +0.2 69,196 - -0.8 70,178 - +0.4
Or see this region's nucleotide sequence