Experiment: KB with Mitomycin C 0.05 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1588 and Psyr_RNA26 are separated by 171 nucleotides Psyr_RNA26 and Psyr_RNA27 are separated by 21 nucleotides Psyr_RNA27 and Psyr_1589 are separated by 360 nucleotides Psyr_1589 and Psyr_1590 are separated by 125 nucleotides
Psyr_1588: Psyr_1588 - Putative exonuclease, RdgC, at 1,787,124 to 1,788,044
_1588
Psyr_RNA26: Psyr_RNA26 - tRNA-Val, at 1,788,216 to 1,788,291
_RNA26
Psyr_RNA27: Psyr_RNA27 - tRNA-Asp, at 1,788,313 to 1,788,389
_RNA27
Psyr_1589: Psyr_1589 - conserved hypothetical protein, at 1,788,750 to 1,789,145
_1589
Psyr_1590: Psyr_1590 - L-carnitine dehydratase/bile acid-inducible protein F, at 1,789,271 to 1,790,461
_1590
Position (kb)
1788
1789 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 1787.557 kb on - strand, within Psyr_1588 at 1787.557 kb on - strand, within Psyr_1588 at 1787.746 kb on + strand, within Psyr_1588 at 1787.746 kb on + strand, within Psyr_1588 at 1787.874 kb on + strand, within Psyr_1588 at 1787.875 kb on - strand, within Psyr_1588 at 1787.920 kb on + strand, within Psyr_1588 at 1787.921 kb on - strand, within Psyr_1588 at 1788.063 kb on + strand at 1788.063 kb on + strand at 1788.063 kb on + strand at 1788.064 kb on - strand at 1788.064 kb on - strand at 1788.064 kb on - strand at 1788.066 kb on - strand at 1788.066 kb on - strand at 1788.081 kb on - strand at 1788.143 kb on - strand at 1788.511 kb on - strand at 1788.545 kb on + strand at 1788.546 kb on - strand at 1788.550 kb on - strand at 1788.612 kb on + strand at 1788.627 kb on + strand at 1788.630 kb on + strand at 1788.686 kb on + strand at 1788.725 kb on + strand at 1788.750 kb on - strand at 1788.776 kb on - strand at 1788.823 kb on + strand, within Psyr_1589 at 1788.858 kb on - strand, within Psyr_1589 at 1788.897 kb on + strand, within Psyr_1589 at 1788.897 kb on + strand, within Psyr_1589 at 1788.898 kb on - strand, within Psyr_1589 at 1788.927 kb on + strand, within Psyr_1589 at 1788.927 kb on + strand, within Psyr_1589 at 1788.929 kb on + strand, within Psyr_1589 at 1788.929 kb on + strand, within Psyr_1589 at 1788.930 kb on - strand, within Psyr_1589 at 1789.069 kb on + strand, within Psyr_1589 at 1789.070 kb on - strand, within Psyr_1589 at 1789.080 kb on - strand, within Psyr_1589 at 1789.080 kb on - strand, within Psyr_1589 at 1789.080 kb on - strand, within Psyr_1589 at 1789.139 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.05 ug/ml remove 1,787,557 - Psyr_1588 0.47 -1.2 1,787,557 - Psyr_1588 0.47 -1.5 1,787,746 + Psyr_1588 0.68 -2.4 1,787,746 + Psyr_1588 0.68 -2.3 1,787,874 + Psyr_1588 0.81 +0.8 1,787,875 - Psyr_1588 0.82 -0.4 1,787,920 + Psyr_1588 0.86 -2.2 1,787,921 - Psyr_1588 0.87 -1.7 1,788,063 + +0.8 1,788,063 + +3.4 1,788,063 + +0.8 1,788,064 - +1.8 1,788,064 - +1.6 1,788,064 - +3.6 1,788,066 - +2.1 1,788,066 - -3.2 1,788,081 - -1.7 1,788,143 - -2.6 1,788,511 - +4.3 1,788,545 + -4.0 1,788,546 - +1.2 1,788,550 - -1.7 1,788,612 + +0.2 1,788,627 + +0.4 1,788,630 + +0.7 1,788,686 + +5.0 1,788,725 + +0.6 1,788,750 - +2.4 1,788,776 - -0.4 1,788,823 + Psyr_1589 0.18 +1.8 1,788,858 - Psyr_1589 0.27 +2.8 1,788,897 + Psyr_1589 0.37 -1.2 1,788,897 + Psyr_1589 0.37 +2.4 1,788,898 - Psyr_1589 0.37 -1.3 1,788,927 + Psyr_1589 0.45 -2.0 1,788,927 + Psyr_1589 0.45 -3.2 1,788,929 + Psyr_1589 0.45 +3.8 1,788,929 + Psyr_1589 0.45 -0.2 1,788,930 - Psyr_1589 0.45 +1.4 1,789,069 + Psyr_1589 0.81 +0.4 1,789,070 - Psyr_1589 0.81 +2.4 1,789,080 - Psyr_1589 0.83 +1.8 1,789,080 - Psyr_1589 0.83 +2.1 1,789,080 - Psyr_1589 0.83 -2.0 1,789,139 + +0.6
Or see this region's nucleotide sequence