Experiment: KB with Mitomycin C 0.05 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4133 and Psyr_4134 are separated by 123 nucleotides Psyr_4134 and Psyr_4135 are separated by 153 nucleotides Psyr_4135 and Psyr_4136 are separated by 22 nucleotides Psyr_4136 and Psyr_4137 are separated by 115 nucleotides Psyr_4137 and Psyr_4138 overlap by 4 nucleotides
Psyr_4133: Psyr_4133 - histidinol dehydrogenase, at 4,923,796 to 4,925,142
_4133
Psyr_4134: Psyr_4134 - ATP phosphoribosyltransferase (homohexameric), at 4,925,266 to 4,925,901
_4134
Psyr_4135: Psyr_4135 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase, at 4,926,055 to 4,927,320
_4135
Psyr_4136: Psyr_4136 - BolA-like protein, at 4,927,343 to 4,927,582
_4136
Psyr_4137: Psyr_4137 - Sulfate transporter/antisigma-factor antagonist STAS, at 4,927,698 to 4,928,003
_4137
Psyr_4138: Psyr_4138 - Toluene tolerance, at 4,928,000 to 4,928,650
_4138
Position (kb) 4926
4927
4928 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 4925.156 kb on + strand at 4925.156 kb on + strand at 4925.157 kb on - strand at 4925.185 kb on + strand at 4925.185 kb on + strand at 4925.194 kb on - strand at 4925.492 kb on + strand, within Psyr_4134 at 4925.492 kb on + strand, within Psyr_4134 at 4925.493 kb on - strand, within Psyr_4134 at 4925.493 kb on - strand, within Psyr_4134 at 4925.493 kb on - strand, within Psyr_4134 at 4925.493 kb on - strand, within Psyr_4134 at 4925.532 kb on - strand, within Psyr_4134 at 4925.532 kb on - strand, within Psyr_4134 at 4925.534 kb on + strand, within Psyr_4134 at 4925.534 kb on + strand, within Psyr_4134 at 4925.535 kb on - strand, within Psyr_4134 at 4925.654 kb on + strand, within Psyr_4134 at 4925.654 kb on + strand, within Psyr_4134 at 4925.654 kb on + strand, within Psyr_4134 at 4925.654 kb on + strand, within Psyr_4134 at 4925.655 kb on - strand, within Psyr_4134 at 4925.672 kb on + strand, within Psyr_4134 at 4925.672 kb on + strand, within Psyr_4134 at 4925.672 kb on + strand, within Psyr_4134 at 4925.673 kb on - strand, within Psyr_4134 at 4925.673 kb on - strand, within Psyr_4134 at 4925.673 kb on - strand, within Psyr_4134 at 4925.673 kb on - strand, within Psyr_4134 at 4925.729 kb on + strand, within Psyr_4134 at 4925.729 kb on + strand, within Psyr_4134 at 4925.729 kb on + strand, within Psyr_4134 at 4925.729 kb on + strand, within Psyr_4134 at 4925.729 kb on + strand, within Psyr_4134 at 4925.729 kb on + strand, within Psyr_4134 at 4925.729 kb on + strand, within Psyr_4134 at 4925.730 kb on - strand, within Psyr_4134 at 4925.730 kb on - strand, within Psyr_4134 at 4925.730 kb on - strand, within Psyr_4134 at 4925.730 kb on - strand, within Psyr_4134 at 4925.730 kb on - strand, within Psyr_4134 at 4925.730 kb on - strand, within Psyr_4134 at 4925.730 kb on - strand, within Psyr_4134 at 4925.730 kb on - strand, within Psyr_4134 at 4925.910 kb on + strand at 4925.910 kb on + strand at 4925.910 kb on + strand at 4925.911 kb on - strand at 4925.911 kb on - strand at 4927.435 kb on - strand, within Psyr_4136 at 4927.435 kb on - strand, within Psyr_4136 at 4927.572 kb on - strand at 4927.627 kb on - strand at 4927.645 kb on - strand at 4927.741 kb on - strand, within Psyr_4137 at 4927.741 kb on - strand, within Psyr_4137 at 4928.033 kb on - strand at 4928.202 kb on + strand, within Psyr_4138 at 4928.202 kb on + strand, within Psyr_4138 at 4928.203 kb on - strand, within Psyr_4138 at 4928.203 kb on - strand, within Psyr_4138 at 4928.214 kb on + strand, within Psyr_4138
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.05 ug/ml remove 4,925,156 + +0.0 4,925,156 + -0.7 4,925,157 - +0.6 4,925,185 + -0.3 4,925,185 + -1.7 4,925,194 - +2.4 4,925,492 + Psyr_4134 0.36 +3.6 4,925,492 + Psyr_4134 0.36 -0.6 4,925,493 - Psyr_4134 0.36 -0.3 4,925,493 - Psyr_4134 0.36 +0.8 4,925,493 - Psyr_4134 0.36 +0.9 4,925,493 - Psyr_4134 0.36 -0.5 4,925,532 - Psyr_4134 0.42 +3.3 4,925,532 - Psyr_4134 0.42 -0.9 4,925,534 + Psyr_4134 0.42 -2.7 4,925,534 + Psyr_4134 0.42 -1.3 4,925,535 - Psyr_4134 0.42 +3.6 4,925,654 + Psyr_4134 0.61 +0.4 4,925,654 + Psyr_4134 0.61 +2.8 4,925,654 + Psyr_4134 0.61 +0.1 4,925,654 + Psyr_4134 0.61 +1.3 4,925,655 - Psyr_4134 0.61 -0.4 4,925,672 + Psyr_4134 0.64 +1.8 4,925,672 + Psyr_4134 0.64 -0.8 4,925,672 + Psyr_4134 0.64 -2.5 4,925,673 - Psyr_4134 0.64 -1.6 4,925,673 - Psyr_4134 0.64 +0.6 4,925,673 - Psyr_4134 0.64 -1.3 4,925,673 - Psyr_4134 0.64 +0.8 4,925,729 + Psyr_4134 0.73 -0.3 4,925,729 + Psyr_4134 0.73 -1.4 4,925,729 + Psyr_4134 0.73 +0.5 4,925,729 + Psyr_4134 0.73 -1.3 4,925,729 + Psyr_4134 0.73 +2.8 4,925,729 + Psyr_4134 0.73 -0.2 4,925,729 + Psyr_4134 0.73 -1.5 4,925,730 - Psyr_4134 0.73 +0.8 4,925,730 - Psyr_4134 0.73 +0.2 4,925,730 - Psyr_4134 0.73 +0.5 4,925,730 - Psyr_4134 0.73 -1.4 4,925,730 - Psyr_4134 0.73 -3.5 4,925,730 - Psyr_4134 0.73 -0.2 4,925,730 - Psyr_4134 0.73 +1.5 4,925,730 - Psyr_4134 0.73 +0.3 4,925,910 + +0.5 4,925,910 + +0.4 4,925,910 + -0.3 4,925,911 - -1.4 4,925,911 - +2.8 4,927,435 - Psyr_4136 0.38 +1.4 4,927,435 - Psyr_4136 0.38 -1.3 4,927,572 - +3.2 4,927,627 - +0.8 4,927,645 - +0.2 4,927,741 - Psyr_4137 0.14 -1.5 4,927,741 - Psyr_4137 0.14 -4.2 4,928,033 - -2.2 4,928,202 + Psyr_4138 0.31 -3.7 4,928,202 + Psyr_4138 0.31 -1.5 4,928,203 - Psyr_4138 0.31 -2.2 4,928,203 - Psyr_4138 0.31 +0.6 4,928,214 + Psyr_4138 0.33 -1.9
Or see this region's nucleotide sequence