Experiment: KB with Mitomycin C 0.05 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3852 and Psyr_3853 are separated by 147 nucleotides Psyr_3853 and Psyr_3854 are separated by 74 nucleotides Psyr_3854 and Psyr_3855 are separated by 153 nucleotides
Psyr_3852: Psyr_3852 - lipoprotein SlyB, putative, at 4,591,592 to 4,592,056
_3852
Psyr_3853: Psyr_3853 - Pyridoxamine 5'-phosphate oxidase, at 4,592,204 to 4,592,851
_3853
Psyr_3854: Psyr_3854 - OmpA/MotB, at 4,592,926 to 4,594,086
_3854
Psyr_3855: Psyr_3855 - Beta-lactamase, at 4,594,240 to 4,595,388
_3855
Position (kb) 4592
4593
4594
4595 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 4591.944 kb on - strand, within Psyr_3852 at 4591.968 kb on + strand, within Psyr_3852 at 4591.993 kb on + strand, within Psyr_3852 at 4592.056 kb on + strand at 4592.056 kb on + strand at 4592.088 kb on + strand at 4592.134 kb on - strand at 4592.152 kb on + strand at 4592.167 kb on + strand at 4592.167 kb on + strand at 4592.196 kb on + strand at 4592.197 kb on - strand at 4592.197 kb on - strand at 4592.927 kb on + strand at 4592.927 kb on + strand at 4592.927 kb on + strand at 4592.927 kb on + strand at 4592.927 kb on + strand at 4592.927 kb on + strand at 4592.927 kb on + strand at 4592.928 kb on - strand at 4592.928 kb on - strand at 4592.928 kb on - strand at 4592.928 kb on - strand at 4592.928 kb on - strand at 4593.688 kb on - strand, within Psyr_3854 at 4594.075 kb on - strand at 4594.075 kb on - strand at 4594.124 kb on + strand at 4594.125 kb on - strand at 4594.137 kb on - strand at 4594.138 kb on + strand at 4594.148 kb on - strand at 4594.148 kb on - strand at 4594.200 kb on - strand at 4594.228 kb on + strand at 4594.228 kb on + strand at 4594.228 kb on + strand at 4594.229 kb on - strand at 4594.229 kb on - strand at 4594.242 kb on - strand at 4594.301 kb on + strand at 4594.301 kb on + strand at 4594.314 kb on + strand at 4594.314 kb on + strand at 4594.315 kb on - strand at 4594.315 kb on - strand at 4594.413 kb on - strand, within Psyr_3855 at 4594.565 kb on + strand, within Psyr_3855 at 4594.565 kb on + strand, within Psyr_3855 at 4594.907 kb on + strand, within Psyr_3855 at 4594.907 kb on + strand, within Psyr_3855 at 4594.907 kb on + strand, within Psyr_3855 at 4594.907 kb on + strand, within Psyr_3855 at 4594.907 kb on + strand, within Psyr_3855 at 4594.908 kb on - strand, within Psyr_3855 at 4594.908 kb on - strand, within Psyr_3855 at 4594.908 kb on - strand, within Psyr_3855 at 4594.908 kb on - strand, within Psyr_3855 at 4594.994 kb on + strand, within Psyr_3855 at 4595.070 kb on + strand, within Psyr_3855 at 4595.071 kb on - strand, within Psyr_3855
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.05 ug/ml remove 4,591,944 - Psyr_3852 0.76 -1.6 4,591,968 + Psyr_3852 0.81 +3.4 4,591,993 + Psyr_3852 0.86 +3.4 4,592,056 + +2.8 4,592,056 + -2.4 4,592,088 + -2.8 4,592,134 - -0.6 4,592,152 + +2.4 4,592,167 + +0.6 4,592,167 + -2.0 4,592,196 + -3.5 4,592,197 - -0.3 4,592,197 - +4.1 4,592,927 + -3.5 4,592,927 + -1.3 4,592,927 + +0.1 4,592,927 + +4.1 4,592,927 + +0.8 4,592,927 + +3.6 4,592,927 + +3.1 4,592,928 - +3.4 4,592,928 - -1.3 4,592,928 - -1.8 4,592,928 - +0.5 4,592,928 - -3.4 4,593,688 - Psyr_3854 0.66 -0.3 4,594,075 - +3.1 4,594,075 - -1.4 4,594,124 + -0.4 4,594,125 - -0.6 4,594,137 - -0.3 4,594,138 + +1.8 4,594,148 - +3.1 4,594,148 - +0.0 4,594,200 - -0.7 4,594,228 + -0.3 4,594,228 + -1.9 4,594,228 + -3.5 4,594,229 - -0.4 4,594,229 - +1.0 4,594,242 - -2.0 4,594,301 + +3.8 4,594,301 + -1.6 4,594,314 + +1.3 4,594,314 + +0.8 4,594,315 - -1.9 4,594,315 - +0.2 4,594,413 - Psyr_3855 0.15 +1.2 4,594,565 + Psyr_3855 0.28 -2.0 4,594,565 + Psyr_3855 0.28 +0.8 4,594,907 + Psyr_3855 0.58 +3.8 4,594,907 + Psyr_3855 0.58 +0.3 4,594,907 + Psyr_3855 0.58 -1.5 4,594,907 + Psyr_3855 0.58 +0.2 4,594,907 + Psyr_3855 0.58 -1.4 4,594,908 - Psyr_3855 0.58 +1.3 4,594,908 - Psyr_3855 0.58 +3.4 4,594,908 - Psyr_3855 0.58 +0.8 4,594,908 - Psyr_3855 0.58 -2.4 4,594,994 + Psyr_3855 0.66 +0.8 4,595,070 + Psyr_3855 0.72 -1.3 4,595,071 - Psyr_3855 0.72 +0.8
Or see this region's nucleotide sequence