Experiment: KB with Mitomycin C 0.05 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0458 and Psyr_0459 are separated by 311 nucleotides Psyr_0459 and Psyr_0460 are separated by 189 nucleotides
Psyr_0458: Psyr_0458 - Helicase, C-terminal:Type III restriction enzyme, res subunit:DEAD/DEAH box helicase, N-terminal, at 494,944 to 496,833
_0458
Psyr_0459: Psyr_0459 - 5,10-methylenetetrahydrofolate reductase, at 497,145 to 497,990
_0459
Psyr_0460: Psyr_0460 - adenosylhomocysteinase, at 498,180 to 499,589
_0460
Position (kb) 497
498 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 496.659 kb on + strand at 496.660 kb on - strand at 496.772 kb on + strand at 496.773 kb on - strand at 496.837 kb on + strand at 496.837 kb on + strand at 496.837 kb on + strand at 496.838 kb on - strand at 496.838 kb on - strand at 496.858 kb on + strand at 496.858 kb on + strand at 496.896 kb on + strand at 497.042 kb on + strand at 497.043 kb on - strand at 497.044 kb on + strand at 497.061 kb on + strand at 497.089 kb on + strand at 497.089 kb on + strand at 497.089 kb on + strand at 497.090 kb on - strand at 497.090 kb on - strand at 497.380 kb on + strand, within Psyr_0459 at 497.380 kb on + strand, within Psyr_0459 at 497.380 kb on + strand, within Psyr_0459 at 497.381 kb on - strand, within Psyr_0459 at 497.381 kb on - strand, within Psyr_0459 at 497.381 kb on - strand, within Psyr_0459 at 497.381 kb on - strand, within Psyr_0459 at 497.381 kb on - strand, within Psyr_0459 at 497.381 kb on - strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.458 kb on + strand, within Psyr_0459 at 497.459 kb on - strand, within Psyr_0459 at 497.459 kb on - strand, within Psyr_0459 at 497.459 kb on - strand, within Psyr_0459 at 497.459 kb on - strand, within Psyr_0459 at 497.459 kb on - strand, within Psyr_0459 at 497.459 kb on - strand, within Psyr_0459 at 497.459 kb on - strand, within Psyr_0459 at 497.459 kb on - strand, within Psyr_0459 at 497.459 kb on - strand, within Psyr_0459 at 497.459 kb on - strand, within Psyr_0459 at 497.479 kb on + strand, within Psyr_0459 at 497.480 kb on - strand, within Psyr_0459 at 497.480 kb on - strand, within Psyr_0459 at 497.480 kb on - strand, within Psyr_0459 at 497.480 kb on - strand, within Psyr_0459 at 497.728 kb on + strand, within Psyr_0459 at 497.728 kb on + strand, within Psyr_0459 at 497.728 kb on + strand, within Psyr_0459 at 497.728 kb on + strand, within Psyr_0459 at 497.728 kb on + strand, within Psyr_0459 at 497.829 kb on - strand, within Psyr_0459 at 497.829 kb on - strand, within Psyr_0459 at 497.866 kb on + strand, within Psyr_0459 at 497.866 kb on + strand, within Psyr_0459 at 497.866 kb on + strand, within Psyr_0459 at 497.867 kb on - strand, within Psyr_0459 at 497.867 kb on - strand, within Psyr_0459 at 497.867 kb on - strand, within Psyr_0459 at 497.867 kb on - strand, within Psyr_0459 at 497.867 kb on - strand, within Psyr_0459 at 497.867 kb on - strand, within Psyr_0459 at 497.867 kb on - strand, within Psyr_0459 at 497.893 kb on + strand, within Psyr_0459 at 497.893 kb on + strand, within Psyr_0459 at 497.893 kb on + strand, within Psyr_0459 at 497.894 kb on - strand, within Psyr_0459 at 497.894 kb on - strand, within Psyr_0459 at 497.894 kb on - strand, within Psyr_0459 at 497.894 kb on - strand, within Psyr_0459 at 497.894 kb on - strand, within Psyr_0459 at 497.894 kb on - strand, within Psyr_0459 at 497.894 kb on - strand, within Psyr_0459 at 497.894 kb on - strand, within Psyr_0459 at 497.894 kb on - strand, within Psyr_0459 at 498.014 kb on - strand at 498.014 kb on - strand at 498.024 kb on + strand at 498.024 kb on + strand at 498.024 kb on + strand at 498.025 kb on - strand at 498.044 kb on + strand at 498.045 kb on - strand at 498.045 kb on - strand at 498.045 kb on - strand at 498.045 kb on - strand at 498.087 kb on + strand at 498.087 kb on + strand at 498.090 kb on + strand at 498.174 kb on + strand at 498.174 kb on + strand at 498.174 kb on + strand at 498.175 kb on - strand at 498.175 kb on - strand at 498.178 kb on + strand at 498.178 kb on + strand at 498.178 kb on + strand at 498.179 kb on - strand at 498.179 kb on - strand at 498.179 kb on - strand at 498.179 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.05 ug/ml remove 496,659 + +1.2 496,660 - -2.7 496,772 + -0.9 496,773 - -1.5 496,837 + -0.2 496,837 + -0.0 496,837 + +1.8 496,838 - -1.1 496,838 - -2.7 496,858 + -0.4 496,858 + -2.0 496,896 + -2.8 497,042 + -1.4 497,043 - +2.3 497,044 + +3.6 497,061 + -1.6 497,089 + +2.8 497,089 + +0.3 497,089 + +0.8 497,090 - -1.1 497,090 - +0.0 497,380 + Psyr_0459 0.28 +0.4 497,380 + Psyr_0459 0.28 -2.0 497,380 + Psyr_0459 0.28 +1.1 497,381 - Psyr_0459 0.28 +1.7 497,381 - Psyr_0459 0.28 +1.0 497,381 - Psyr_0459 0.28 -0.1 497,381 - Psyr_0459 0.28 -0.5 497,381 - Psyr_0459 0.28 +0.4 497,381 - Psyr_0459 0.28 -1.6 497,458 + Psyr_0459 0.37 -0.5 497,458 + Psyr_0459 0.37 -2.8 497,458 + Psyr_0459 0.37 -1.9 497,458 + Psyr_0459 0.37 +1.2 497,458 + Psyr_0459 0.37 +1.2 497,459 - Psyr_0459 0.37 -0.5 497,459 - Psyr_0459 0.37 +0.8 497,459 - Psyr_0459 0.37 -2.4 497,459 - Psyr_0459 0.37 -2.1 497,459 - Psyr_0459 0.37 -0.0 497,459 - Psyr_0459 0.37 -2.9 497,459 - Psyr_0459 0.37 +3.3 497,459 - Psyr_0459 0.37 -0.3 497,459 - Psyr_0459 0.37 -0.6 497,459 - Psyr_0459 0.37 -3.2 497,479 + Psyr_0459 0.39 +0.8 497,480 - Psyr_0459 0.40 -0.1 497,480 - Psyr_0459 0.40 +0.4 497,480 - Psyr_0459 0.40 -2.5 497,480 - Psyr_0459 0.40 -2.1 497,728 + Psyr_0459 0.69 -0.8 497,728 + Psyr_0459 0.69 +0.2 497,728 + Psyr_0459 0.69 +0.4 497,728 + Psyr_0459 0.69 +0.4 497,728 + Psyr_0459 0.69 +1.8 497,829 - Psyr_0459 0.81 -1.1 497,829 - Psyr_0459 0.81 +3.8 497,866 + Psyr_0459 0.85 -1.2 497,866 + Psyr_0459 0.85 -0.0 497,866 + Psyr_0459 0.85 -1.7 497,867 - Psyr_0459 0.85 -1.1 497,867 - Psyr_0459 0.85 +5.6 497,867 - Psyr_0459 0.85 +2.8 497,867 - Psyr_0459 0.85 +1.4 497,867 - Psyr_0459 0.85 +2.2 497,867 - Psyr_0459 0.85 -1.7 497,867 - Psyr_0459 0.85 -0.9 497,893 + Psyr_0459 0.88 -1.2 497,893 + Psyr_0459 0.88 -1.1 497,893 + Psyr_0459 0.88 -1.8 497,894 - Psyr_0459 0.89 -3.4 497,894 - Psyr_0459 0.89 -2.2 497,894 - Psyr_0459 0.89 -2.0 497,894 - Psyr_0459 0.89 -1.0 497,894 - Psyr_0459 0.89 -0.5 497,894 - Psyr_0459 0.89 -1.8 497,894 - Psyr_0459 0.89 -1.6 497,894 - Psyr_0459 0.89 -2.3 497,894 - Psyr_0459 0.89 -1.7 498,014 - +0.6 498,014 - -0.6 498,024 + +0.4 498,024 + -1.1 498,024 + +1.5 498,025 - +0.8 498,044 + -0.6 498,045 - +4.1 498,045 - -0.9 498,045 - -1.9 498,045 - -1.4 498,087 + -1.0 498,087 + -2.9 498,090 + +0.5 498,174 + +4.1 498,174 + +1.4 498,174 + +0.4 498,175 - +0.2 498,175 - +0.8 498,178 + +1.8 498,178 + +1.8 498,178 + +1.8 498,179 - -0.3 498,179 - -4.1 498,179 - -1.9 498,179 - -1.5
Or see this region's nucleotide sequence