Experiment: KB with Mitomycin C 0.05 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0356 and Psyr_0357 are separated by 3 nucleotides Psyr_0357 and Psyr_0358 overlap by 4 nucleotides Psyr_0358 and Psyr_0359 are separated by 219 nucleotides
Psyr_0356: Psyr_0356 - L-cystine ABC transporter ATP-binding protein / Diaminopimelate ABC transporter ATP-binding protein, at 381,987 to 382,730
_0356
Psyr_0357: Psyr_0357 - Diaminopimelate ABC transporter membrane protein / L-cystine ABC transporter membrane protein, at 382,734 to 383,402
_0357
Psyr_0358: Psyr_0358 - Diaminopimelate-binding protein / L-cystine-binding protein, at 383,399 to 384,196
_0358
Psyr_0359: Psyr_0359 - D-cysteine desulfhydrase, at 384,416 to 385,414
_0359
Position (kb)
383
384
385 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 382.725 kb on + strand at 382.725 kb on + strand at 382.726 kb on - strand at 382.726 kb on - strand at 382.813 kb on + strand, within Psyr_0357 at 382.814 kb on - strand, within Psyr_0357 at 382.814 kb on - strand, within Psyr_0357 at 382.834 kb on + strand, within Psyr_0357 at 382.834 kb on + strand, within Psyr_0357 at 382.834 kb on + strand, within Psyr_0357 at 382.834 kb on + strand, within Psyr_0357 at 382.834 kb on + strand, within Psyr_0357 at 382.834 kb on + strand, within Psyr_0357 at 382.834 kb on + strand, within Psyr_0357 at 382.835 kb on - strand, within Psyr_0357 at 382.835 kb on - strand, within Psyr_0357 at 382.835 kb on - strand, within Psyr_0357 at 382.835 kb on - strand, within Psyr_0357 at 382.835 kb on - strand, within Psyr_0357 at 383.095 kb on + strand, within Psyr_0357 at 383.096 kb on - strand, within Psyr_0357 at 383.176 kb on + strand, within Psyr_0357 at 383.176 kb on + strand, within Psyr_0357 at 383.177 kb on - strand, within Psyr_0357 at 383.177 kb on - strand, within Psyr_0357 at 383.177 kb on - strand, within Psyr_0357 at 383.177 kb on - strand, within Psyr_0357 at 383.227 kb on + strand, within Psyr_0357 at 383.227 kb on + strand, within Psyr_0357 at 383.227 kb on + strand, within Psyr_0357 at 383.227 kb on + strand, within Psyr_0357 at 383.227 kb on + strand, within Psyr_0357 at 383.227 kb on + strand, within Psyr_0357 at 383.227 kb on + strand, within Psyr_0357 at 383.227 kb on + strand, within Psyr_0357 at 383.227 kb on + strand, within Psyr_0357 at 383.228 kb on - strand, within Psyr_0357 at 383.228 kb on - strand, within Psyr_0357 at 383.228 kb on - strand, within Psyr_0357 at 383.228 kb on - strand, within Psyr_0357 at 383.228 kb on - strand, within Psyr_0357 at 383.228 kb on - strand, within Psyr_0357 at 383.228 kb on - strand, within Psyr_0357 at 383.228 kb on - strand, within Psyr_0357 at 383.331 kb on + strand, within Psyr_0357 at 383.338 kb on + strand at 383.338 kb on + strand at 383.339 kb on - strand at 383.339 kb on - strand at 383.342 kb on - strand at 383.342 kb on - strand at 383.424 kb on + strand at 383.636 kb on + strand, within Psyr_0358 at 383.636 kb on + strand, within Psyr_0358 at 383.636 kb on + strand, within Psyr_0358 at 383.637 kb on - strand, within Psyr_0358 at 383.637 kb on - strand, within Psyr_0358 at 383.637 kb on - strand, within Psyr_0358 at 383.637 kb on - strand, within Psyr_0358 at 383.637 kb on - strand, within Psyr_0358 at 383.637 kb on - strand, within Psyr_0358 at 383.673 kb on + strand, within Psyr_0358 at 383.673 kb on + strand, within Psyr_0358 at 383.674 kb on - strand, within Psyr_0358 at 383.674 kb on - strand, within Psyr_0358 at 383.721 kb on + strand, within Psyr_0358 at 383.721 kb on + strand, within Psyr_0358 at 383.721 kb on + strand, within Psyr_0358 at 383.722 kb on - strand, within Psyr_0358 at 383.722 kb on - strand, within Psyr_0358 at 383.829 kb on + strand, within Psyr_0358 at 383.829 kb on + strand, within Psyr_0358 at 383.829 kb on + strand, within Psyr_0358 at 383.829 kb on + strand, within Psyr_0358 at 383.847 kb on + strand, within Psyr_0358 at 383.847 kb on + strand, within Psyr_0358 at 383.847 kb on + strand, within Psyr_0358 at 383.847 kb on + strand, within Psyr_0358 at 384.048 kb on + strand, within Psyr_0358 at 384.048 kb on + strand, within Psyr_0358 at 384.049 kb on - strand, within Psyr_0358 at 384.049 kb on - strand, within Psyr_0358 at 384.049 kb on - strand, within Psyr_0358 at 384.049 kb on - strand, within Psyr_0358 at 384.173 kb on - strand at 384.265 kb on + strand at 384.287 kb on + strand at 384.287 kb on + strand at 384.287 kb on + strand at 384.288 kb on - strand at 384.288 kb on - strand at 384.298 kb on + strand at 384.298 kb on + strand at 384.298 kb on + strand at 384.299 kb on - strand at 384.331 kb on + strand at 384.331 kb on + strand at 384.331 kb on + strand at 384.332 kb on - strand at 384.332 kb on - strand at 384.333 kb on + strand at 384.333 kb on + strand at 384.333 kb on + strand at 384.333 kb on + strand at 384.333 kb on + strand at 384.333 kb on + strand at 384.333 kb on + strand at 384.333 kb on + strand at 384.333 kb on + strand at 384.334 kb on - strand at 384.334 kb on - strand at 384.335 kb on + strand at 384.335 kb on + strand at 384.335 kb on + strand at 384.335 kb on + strand at 384.336 kb on - strand at 384.336 kb on - strand at 384.348 kb on - strand at 384.546 kb on + strand, within Psyr_0359 at 384.639 kb on + strand, within Psyr_0359 at 384.639 kb on + strand, within Psyr_0359 at 384.639 kb on + strand, within Psyr_0359 at 384.640 kb on - strand, within Psyr_0359 at 384.909 kb on + strand, within Psyr_0359 at 384.909 kb on + strand, within Psyr_0359 at 384.910 kb on - strand, within Psyr_0359 at 385.037 kb on + strand, within Psyr_0359 at 385.037 kb on + strand, within Psyr_0359 at 385.037 kb on + strand, within Psyr_0359 at 385.037 kb on + strand, within Psyr_0359 at 385.037 kb on + strand, within Psyr_0359 at 385.038 kb on - strand, within Psyr_0359 at 385.087 kb on - strand, within Psyr_0359
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mitomycin C 0.05 ug/ml remove 382,725 + -2.8 382,725 + +3.1 382,726 - -0.1 382,726 - -0.7 382,813 + Psyr_0357 0.12 +4.0 382,814 - Psyr_0357 0.12 +0.8 382,814 - Psyr_0357 0.12 +2.3 382,834 + Psyr_0357 0.15 -0.6 382,834 + Psyr_0357 0.15 +1.8 382,834 + Psyr_0357 0.15 -0.2 382,834 + Psyr_0357 0.15 -0.8 382,834 + Psyr_0357 0.15 +1.7 382,834 + Psyr_0357 0.15 +0.1 382,834 + Psyr_0357 0.15 -2.1 382,835 - Psyr_0357 0.15 -0.6 382,835 - Psyr_0357 0.15 -2.0 382,835 - Psyr_0357 0.15 -1.7 382,835 - Psyr_0357 0.15 -0.8 382,835 - Psyr_0357 0.15 +0.1 383,095 + Psyr_0357 0.54 -3.1 383,096 - Psyr_0357 0.54 +0.4 383,176 + Psyr_0357 0.66 +4.0 383,176 + Psyr_0357 0.66 +0.5 383,177 - Psyr_0357 0.66 -2.3 383,177 - Psyr_0357 0.66 -1.4 383,177 - Psyr_0357 0.66 -1.3 383,177 - Psyr_0357 0.66 +0.9 383,227 + Psyr_0357 0.74 +1.8 383,227 + Psyr_0357 0.74 -1.1 383,227 + Psyr_0357 0.74 +2.0 383,227 + Psyr_0357 0.74 +1.0 383,227 + Psyr_0357 0.74 +2.4 383,227 + Psyr_0357 0.74 +0.8 383,227 + Psyr_0357 0.74 -2.0 383,227 + Psyr_0357 0.74 +0.1 383,227 + Psyr_0357 0.74 -2.4 383,228 - Psyr_0357 0.74 -0.9 383,228 - Psyr_0357 0.74 -0.3 383,228 - Psyr_0357 0.74 -0.4 383,228 - Psyr_0357 0.74 -2.5 383,228 - Psyr_0357 0.74 +0.5 383,228 - Psyr_0357 0.74 -0.2 383,228 - Psyr_0357 0.74 -0.5 383,228 - Psyr_0357 0.74 -3.3 383,331 + Psyr_0357 0.89 -0.4 383,338 + -1.1 383,338 + -0.6 383,339 - +2.4 383,339 - -1.1 383,342 - +0.2 383,342 - +0.2 383,424 + -1.5 383,636 + Psyr_0358 0.30 +3.1 383,636 + Psyr_0358 0.30 -2.8 383,636 + Psyr_0358 0.30 -1.2 383,637 - Psyr_0358 0.30 -4.0 383,637 - Psyr_0358 0.30 -0.0 383,637 - Psyr_0358 0.30 +3.6 383,637 - Psyr_0358 0.30 +0.6 383,637 - Psyr_0358 0.30 +0.1 383,637 - Psyr_0358 0.30 +1.7 383,673 + Psyr_0358 0.34 -0.6 383,673 + Psyr_0358 0.34 +3.6 383,674 - Psyr_0358 0.34 -0.6 383,674 - Psyr_0358 0.34 +0.1 383,721 + Psyr_0358 0.40 -0.3 383,721 + Psyr_0358 0.40 -1.1 383,721 + Psyr_0358 0.40 -3.4 383,722 - Psyr_0358 0.40 +1.6 383,722 - Psyr_0358 0.40 -2.5 383,829 + Psyr_0358 0.54 +0.1 383,829 + Psyr_0358 0.54 -2.4 383,829 + Psyr_0358 0.54 -2.2 383,829 + Psyr_0358 0.54 +1.8 383,847 + Psyr_0358 0.56 -2.2 383,847 + Psyr_0358 0.56 -3.7 383,847 + Psyr_0358 0.56 -2.1 383,847 + Psyr_0358 0.56 -1.6 384,048 + Psyr_0358 0.81 +0.2 384,048 + Psyr_0358 0.81 -3.0 384,049 - Psyr_0358 0.81 +0.5 384,049 - Psyr_0358 0.81 +0.1 384,049 - Psyr_0358 0.81 -1.5 384,049 - Psyr_0358 0.81 +0.8 384,173 - -0.4 384,265 + -1.5 384,287 + +0.8 384,287 + +3.8 384,287 + -1.4 384,288 - +4.0 384,288 - -0.0 384,298 + +2.4 384,298 + +4.5 384,298 + +1.3 384,299 - -1.2 384,331 + -0.5 384,331 + +1.2 384,331 + -1.1 384,332 - -2.5 384,332 - +0.8 384,333 + -2.5 384,333 + +3.1 384,333 + +0.7 384,333 + +0.8 384,333 + -1.5 384,333 + +0.8 384,333 + +1.3 384,333 + -1.7 384,333 + -0.4 384,334 - -2.4 384,334 - -0.6 384,335 + -1.0 384,335 + +3.4 384,335 + -2.0 384,335 + -3.0 384,336 - +0.8 384,336 - +2.4 384,348 - -0.4 384,546 + Psyr_0359 0.13 +1.2 384,639 + Psyr_0359 0.22 +3.8 384,639 + Psyr_0359 0.22 +2.8 384,639 + Psyr_0359 0.22 -0.3 384,640 - Psyr_0359 0.22 +1.4 384,909 + Psyr_0359 0.49 +0.4 384,909 + Psyr_0359 0.49 -1.5 384,910 - Psyr_0359 0.49 -2.3 385,037 + Psyr_0359 0.62 +1.8 385,037 + Psyr_0359 0.62 -2.2 385,037 + Psyr_0359 0.62 +0.3 385,037 + Psyr_0359 0.62 -2.6 385,037 + Psyr_0359 0.62 +0.3 385,038 - Psyr_0359 0.62 -0.2 385,087 - Psyr_0359 0.67 +0.7
Or see this region's nucleotide sequence