Experiment: NAG (C); with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS02955 and PFLU_RS02960 are separated by 568 nucleotides PFLU_RS02960 and PFLU_RS02965 are separated by 119 nucleotides PFLU_RS02965 and PFLU_RS02970 overlap by 4 nucleotides
PFLU_RS02955: PFLU_RS02955 - DUF2946 domain-containing protein, at 681,319 to 681,714
_RS02955
PFLU_RS02960: PFLU_RS02960 - hypothetical protein, at 682,283 to 682,531
_RS02960
PFLU_RS02965: PFLU_RS02965 - cobalt-precorrin-6A reductase, at 682,651 to 683,370
_RS02965
PFLU_RS02970: PFLU_RS02970 - bifunctional cobalt-precorrin-7 (C(5))-methyltransferase/cobalt-precorrin-6B (C(15))-methyltransferase, at 683,367 to 684,572
_RS02970
Position (kb) 682
683
684 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 681.728 kb on + strand at 681.750 kb on + strand at 681.750 kb on + strand at 681.750 kb on + strand at 681.750 kb on + strand at 681.751 kb on - strand at 681.751 kb on - strand at 681.754 kb on + strand at 681.755 kb on - strand at 681.755 kb on - strand at 681.776 kb on + strand at 681.776 kb on + strand at 681.776 kb on + strand at 681.776 kb on + strand at 681.777 kb on - strand at 681.823 kb on - strand at 681.983 kb on - strand at 681.983 kb on - strand at 682.013 kb on + strand at 682.014 kb on - strand at 682.026 kb on - strand at 682.041 kb on + strand at 682.042 kb on - strand at 682.042 kb on - strand at 682.050 kb on + strand at 682.135 kb on + strand at 682.135 kb on + strand at 682.136 kb on - strand at 682.148 kb on + strand at 682.148 kb on + strand at 682.169 kb on - strand at 682.169 kb on - strand at 682.179 kb on - strand at 682.217 kb on - strand at 682.264 kb on + strand at 682.264 kb on + strand at 682.270 kb on - strand at 682.544 kb on - strand at 682.571 kb on + strand at 682.571 kb on + strand at 682.586 kb on + strand at 682.586 kb on + strand at 682.586 kb on + strand at 682.586 kb on + strand at 682.586 kb on + strand at 682.586 kb on + strand at 682.587 kb on - strand at 682.587 kb on - strand at 682.587 kb on - strand at 682.587 kb on - strand at 682.587 kb on - strand at 682.587 kb on - strand at 682.587 kb on - strand at 682.587 kb on - strand at 682.588 kb on + strand at 682.588 kb on + strand at 682.588 kb on + strand at 682.588 kb on + strand at 682.588 kb on + strand at 682.588 kb on + strand at 682.588 kb on + strand at 682.588 kb on + strand at 682.588 kb on + strand at 682.588 kb on + strand at 682.588 kb on + strand at 682.588 kb on + strand at 682.588 kb on + strand at 682.588 kb on + strand at 682.589 kb on - strand at 682.589 kb on - strand at 682.589 kb on - strand at 682.589 kb on - strand at 682.589 kb on - strand at 682.589 kb on - strand at 682.589 kb on - strand at 682.589 kb on - strand at 682.589 kb on - strand at 682.589 kb on - strand at 682.589 kb on - strand at 682.605 kb on + strand at 682.605 kb on + strand at 682.605 kb on + strand at 682.606 kb on - strand at 682.606 kb on - strand at 682.606 kb on - strand at 682.636 kb on + strand at 682.636 kb on + strand at 682.636 kb on + strand at 682.636 kb on + strand at 682.637 kb on - strand at 682.637 kb on - strand at 682.637 kb on - strand at 682.673 kb on - strand at 682.791 kb on + strand, within PFLU_RS02965 at 683.156 kb on + strand, within PFLU_RS02965 at 683.157 kb on - strand, within PFLU_RS02965 at 683.157 kb on - strand, within PFLU_RS02965 at 683.184 kb on + strand, within PFLU_RS02965 at 683.184 kb on + strand, within PFLU_RS02965 at 683.185 kb on - strand, within PFLU_RS02965 at 683.231 kb on + strand, within PFLU_RS02965 at 683.292 kb on + strand, within PFLU_RS02965 at 683.293 kb on - strand, within PFLU_RS02965 at 683.294 kb on + strand, within PFLU_RS02965 at 683.294 kb on + strand, within PFLU_RS02965 at 683.294 kb on + strand, within PFLU_RS02965 at 683.294 kb on + strand, within PFLU_RS02965 at 683.294 kb on + strand, within PFLU_RS02965 at 683.294 kb on + strand, within PFLU_RS02965 at 683.294 kb on + strand, within PFLU_RS02965 at 683.294 kb on + strand, within PFLU_RS02965 at 683.294 kb on + strand, within PFLU_RS02965 at 683.294 kb on + strand, within PFLU_RS02965 at 683.294 kb on + strand, within PFLU_RS02965 at 683.295 kb on - strand, within PFLU_RS02965 at 683.295 kb on - strand, within PFLU_RS02965 at 683.295 kb on - strand, within PFLU_RS02965 at 683.295 kb on - strand, within PFLU_RS02965 at 683.295 kb on - strand, within PFLU_RS02965 at 683.295 kb on - strand, within PFLU_RS02965 at 683.295 kb on - strand, within PFLU_RS02965 at 683.295 kb on - strand, within PFLU_RS02965 at 683.295 kb on - strand, within PFLU_RS02965 at 683.295 kb on - strand, within PFLU_RS02965 at 683.295 kb on - strand, within PFLU_RS02965 at 683.295 kb on - strand, within PFLU_RS02965 at 683.295 kb on - strand, within PFLU_RS02965 at 683.295 kb on - strand, within PFLU_RS02965 at 683.295 kb on - strand, within PFLU_RS02965 at 683.295 kb on - strand, within PFLU_RS02965 at 683.298 kb on + strand, within PFLU_RS02965 at 683.298 kb on + strand, within PFLU_RS02965 at 683.298 kb on + strand, within PFLU_RS02965 at 683.298 kb on + strand, within PFLU_RS02965 at 683.298 kb on + strand, within PFLU_RS02965 at 683.298 kb on + strand, within PFLU_RS02965 at 683.298 kb on + strand, within PFLU_RS02965 at 683.298 kb on + strand, within PFLU_RS02965 at 683.298 kb on + strand, within PFLU_RS02965 at 683.298 kb on + strand, within PFLU_RS02965 at 683.298 kb on + strand, within PFLU_RS02965 at 683.298 kb on + strand, within PFLU_RS02965 at 683.299 kb on - strand at 683.299 kb on - strand at 683.299 kb on - strand at 683.299 kb on - strand at 683.299 kb on - strand at 683.299 kb on - strand at 683.299 kb on - strand at 683.299 kb on - strand at 683.299 kb on - strand at 683.299 kb on - strand at 683.299 kb on - strand at 683.299 kb on - strand at 683.299 kb on - strand at 683.299 kb on - strand at 683.299 kb on - strand at 683.299 kb on - strand at 683.316 kb on + strand at 683.316 kb on + strand at 683.316 kb on + strand at 683.316 kb on + strand at 683.316 kb on + strand at 683.316 kb on + strand at 683.317 kb on - strand at 683.371 kb on - strand at 683.371 kb on - strand at 683.420 kb on + strand at 683.420 kb on + strand at 683.421 kb on - strand at 683.556 kb on + strand, within PFLU_RS02970 at 683.556 kb on + strand, within PFLU_RS02970 at 683.557 kb on - strand, within PFLU_RS02970 at 683.573 kb on + strand, within PFLU_RS02970 at 683.897 kb on + strand, within PFLU_RS02970 at 683.942 kb on + strand, within PFLU_RS02970 at 684.075 kb on - strand, within PFLU_RS02970 at 684.331 kb on + strand, within PFLU_RS02970 at 684.331 kb on + strand, within PFLU_RS02970
Per-strain Table
Position Strand Gene LocusTag Fraction NAG (C); with MOPS remove 681,728 + -0.3 681,750 + -0.3 681,750 + -1.3 681,750 + -0.1 681,750 + +1.8 681,751 - +0.9 681,751 - +0.5 681,754 + +0.4 681,755 - -0.1 681,755 - -1.7 681,776 + -0.2 681,776 + +0.2 681,776 + -1.0 681,776 + -0.6 681,777 - -0.7 681,823 - +0.4 681,983 - -0.6 681,983 - -1.2 682,013 + -0.7 682,014 - +1.0 682,026 - +0.3 682,041 + +0.2 682,042 - -1.7 682,042 - +0.8 682,050 + -0.3 682,135 + +1.1 682,135 + -0.2 682,136 - -2.0 682,148 + -0.1 682,148 + +0.2 682,169 - -0.7 682,169 - +0.3 682,179 - +0.0 682,217 - -1.3 682,264 + +1.6 682,264 + +2.0 682,270 - -0.5 682,544 - +0.3 682,571 + +0.9 682,571 + -0.0 682,586 + +1.0 682,586 + -1.0 682,586 + -0.8 682,586 + +0.5 682,586 + +0.8 682,586 + +0.5 682,587 - +0.5 682,587 - -0.6 682,587 - -0.0 682,587 - -0.8 682,587 - -0.0 682,587 - +0.4 682,587 - +0.6 682,587 - -0.7 682,588 + +0.1 682,588 + +1.8 682,588 + +1.1 682,588 + +1.7 682,588 + -0.3 682,588 + +1.8 682,588 + +0.1 682,588 + -2.2 682,588 + -2.4 682,588 + -0.6 682,588 + +0.7 682,588 + -1.5 682,588 + +0.0 682,588 + +0.7 682,589 - -0.3 682,589 - -0.8 682,589 - -2.3 682,589 - +0.7 682,589 - -0.8 682,589 - -0.6 682,589 - +0.4 682,589 - -0.5 682,589 - +0.6 682,589 - -1.8 682,589 - -0.0 682,605 + +0.7 682,605 + -0.4 682,605 + +1.0 682,606 - -1.1 682,606 - +0.1 682,606 - -1.7 682,636 + -1.7 682,636 + +0.1 682,636 + -2.0 682,636 + +0.4 682,637 - +1.0 682,637 - -0.4 682,637 - +0.4 682,673 - -0.9 682,791 + PFLU_RS02965 0.19 +1.2 683,156 + PFLU_RS02965 0.70 -0.0 683,157 - PFLU_RS02965 0.70 -1.8 683,157 - PFLU_RS02965 0.70 -2.2 683,184 + PFLU_RS02965 0.74 -1.2 683,184 + PFLU_RS02965 0.74 -0.3 683,185 - PFLU_RS02965 0.74 -1.3 683,231 + PFLU_RS02965 0.81 +0.2 683,292 + PFLU_RS02965 0.89 -0.6 683,293 - PFLU_RS02965 0.89 -1.3 683,294 + PFLU_RS02965 0.89 -1.5 683,294 + PFLU_RS02965 0.89 -1.4 683,294 + PFLU_RS02965 0.89 -1.1 683,294 + PFLU_RS02965 0.89 -1.7 683,294 + PFLU_RS02965 0.89 -0.7 683,294 + PFLU_RS02965 0.89 -2.5 683,294 + PFLU_RS02965 0.89 -1.2 683,294 + PFLU_RS02965 0.89 -2.9 683,294 + PFLU_RS02965 0.89 -2.5 683,294 + PFLU_RS02965 0.89 -1.0 683,294 + PFLU_RS02965 0.89 +1.1 683,295 - PFLU_RS02965 0.89 -1.0 683,295 - PFLU_RS02965 0.89 -0.4 683,295 - PFLU_RS02965 0.89 -0.6 683,295 - PFLU_RS02965 0.89 -2.5 683,295 - PFLU_RS02965 0.89 -0.1 683,295 - PFLU_RS02965 0.89 -2.4 683,295 - PFLU_RS02965 0.89 -2.7 683,295 - PFLU_RS02965 0.89 -1.1 683,295 - PFLU_RS02965 0.89 -0.7 683,295 - PFLU_RS02965 0.89 -0.6 683,295 - PFLU_RS02965 0.89 -0.8 683,295 - PFLU_RS02965 0.89 -0.3 683,295 - PFLU_RS02965 0.89 -0.4 683,295 - PFLU_RS02965 0.89 -0.6 683,295 - PFLU_RS02965 0.89 +0.2 683,295 - PFLU_RS02965 0.89 -1.8 683,298 + PFLU_RS02965 0.90 -1.7 683,298 + PFLU_RS02965 0.90 -0.5 683,298 + PFLU_RS02965 0.90 -2.1 683,298 + PFLU_RS02965 0.90 -1.6 683,298 + PFLU_RS02965 0.90 +0.6 683,298 + PFLU_RS02965 0.90 -1.3 683,298 + PFLU_RS02965 0.90 -1.0 683,298 + PFLU_RS02965 0.90 -1.3 683,298 + PFLU_RS02965 0.90 -0.9 683,298 + PFLU_RS02965 0.90 -0.9 683,298 + PFLU_RS02965 0.90 +1.8 683,298 + PFLU_RS02965 0.90 +1.2 683,299 - -2.1 683,299 - -0.3 683,299 - -1.0 683,299 - +0.5 683,299 - -3.2 683,299 - -0.8 683,299 - -0.7 683,299 - +0.7 683,299 - +0.4 683,299 - -2.0 683,299 - -1.1 683,299 - +0.7 683,299 - -2.5 683,299 - -1.6 683,299 - -2.7 683,299 - -0.0 683,316 + -0.1 683,316 + -0.8 683,316 + +0.6 683,316 + +0.6 683,316 + -1.0 683,316 + -0.6 683,317 - -1.3 683,371 - -0.6 683,371 - +0.8 683,420 + -0.9 683,420 + -2.1 683,421 - -1.2 683,556 + PFLU_RS02970 0.16 -0.8 683,556 + PFLU_RS02970 0.16 -1.7 683,557 - PFLU_RS02970 0.16 -1.5 683,573 + PFLU_RS02970 0.17 -2.3 683,897 + PFLU_RS02970 0.44 -0.3 683,942 + PFLU_RS02970 0.48 -2.0 684,075 - PFLU_RS02970 0.59 -2.2 684,331 + PFLU_RS02970 0.80 -2.3 684,331 + PFLU_RS02970 0.80 +0.4
Or see this region's nucleotide sequence