Experiment: NAG (C); with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS01635 and PFLU_RS01640 are separated by 88 nucleotides PFLU_RS01640 and PFLU_RS01645 are separated by 1 nucleotides PFLU_RS01645 and PFLU_RS01650 are separated by 30 nucleotides
PFLU_RS01635: PFLU_RS01635 - serine protease, at 364,512 to 366,077
_RS01635
PFLU_RS01640: PFLU_RS01640 - divergent polysaccharide deacetylase family protein, at 366,166 to 366,954
_RS01640
PFLU_RS01645: PFLU_RS01645 - S41 family peptidase, at 366,956 to 368,272
_RS01645
PFLU_RS01650: PFLU_RS01650 - murein hydrolase activator EnvC, at 368,303 to 369,598
_RS01650
Position (kb) 366
367
368
369 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 365.957 kb on + strand at 365.957 kb on + strand at 365.957 kb on + strand at 365.957 kb on + strand at 365.957 kb on + strand at 365.958 kb on - strand at 365.958 kb on - strand at 365.958 kb on - strand at 365.958 kb on - strand at 365.958 kb on - strand at 365.958 kb on - strand at 365.958 kb on - strand at 365.958 kb on - strand at 365.958 kb on - strand at 365.958 kb on - strand at 365.958 kb on - strand at 365.958 kb on - strand at 365.958 kb on - strand at 365.958 kb on - strand at 366.039 kb on + strand at 366.039 kb on + strand at 366.040 kb on - strand at 366.040 kb on - strand at 366.043 kb on + strand at 366.043 kb on + strand at 366.043 kb on + strand at 366.043 kb on + strand at 366.043 kb on + strand at 366.043 kb on + strand at 366.043 kb on + strand at 366.043 kb on + strand at 366.043 kb on + strand at 366.044 kb on - strand at 366.044 kb on - strand at 366.044 kb on - strand at 366.044 kb on - strand at 366.044 kb on - strand at 366.134 kb on + strand at 366.134 kb on + strand at 366.135 kb on - strand at 366.135 kb on - strand at 366.164 kb on - strand at 366.164 kb on - strand at 366.167 kb on + strand at 366.167 kb on + strand at 366.167 kb on + strand at 366.167 kb on + strand at 366.167 kb on + strand at 366.167 kb on + strand at 366.167 kb on + strand at 366.167 kb on + strand at 366.167 kb on + strand at 366.167 kb on + strand at 366.167 kb on + strand at 366.167 kb on + strand at 366.167 kb on + strand at 366.167 kb on + strand at 366.168 kb on - strand at 366.168 kb on - strand at 366.168 kb on - strand at 366.168 kb on - strand at 366.168 kb on - strand at 366.168 kb on - strand at 366.168 kb on - strand at 366.168 kb on - strand at 366.168 kb on - strand at 366.168 kb on - strand at 366.168 kb on - strand at 366.168 kb on - strand at 366.168 kb on - strand at 366.168 kb on - strand at 366.168 kb on - strand at 366.171 kb on + strand at 366.189 kb on - strand at 366.189 kb on - strand at 366.253 kb on - strand, within PFLU_RS01640 at 366.393 kb on + strand, within PFLU_RS01640 at 366.607 kb on - strand, within PFLU_RS01640 at 366.608 kb on + strand, within PFLU_RS01640 at 366.608 kb on + strand, within PFLU_RS01640 at 366.608 kb on + strand, within PFLU_RS01640 at 366.955 kb on - strand at 366.955 kb on - strand at 367.014 kb on + strand at 367.119 kb on + strand, within PFLU_RS01645 at 367.119 kb on + strand, within PFLU_RS01645 at 367.119 kb on + strand, within PFLU_RS01645 at 367.119 kb on + strand, within PFLU_RS01645 at 367.119 kb on + strand, within PFLU_RS01645 at 367.119 kb on + strand, within PFLU_RS01645 at 367.119 kb on + strand, within PFLU_RS01645 at 367.119 kb on + strand, within PFLU_RS01645 at 367.119 kb on + strand, within PFLU_RS01645 at 367.120 kb on - strand, within PFLU_RS01645 at 367.120 kb on - strand, within PFLU_RS01645 at 367.122 kb on + strand, within PFLU_RS01645 at 367.122 kb on + strand, within PFLU_RS01645 at 367.122 kb on + strand, within PFLU_RS01645 at 367.122 kb on + strand, within PFLU_RS01645 at 367.215 kb on + strand, within PFLU_RS01645 at 367.215 kb on + strand, within PFLU_RS01645 at 367.215 kb on + strand, within PFLU_RS01645 at 367.216 kb on - strand, within PFLU_RS01645 at 367.216 kb on - strand, within PFLU_RS01645 at 367.216 kb on - strand, within PFLU_RS01645 at 367.216 kb on - strand, within PFLU_RS01645 at 367.218 kb on + strand, within PFLU_RS01645 at 367.218 kb on + strand, within PFLU_RS01645 at 367.218 kb on + strand, within PFLU_RS01645 at 367.218 kb on + strand, within PFLU_RS01645 at 367.219 kb on - strand, within PFLU_RS01645 at 367.219 kb on - strand, within PFLU_RS01645 at 367.464 kb on + strand, within PFLU_RS01645 at 367.465 kb on - strand, within PFLU_RS01645 at 367.537 kb on + strand, within PFLU_RS01645 at 367.650 kb on + strand, within PFLU_RS01645 at 367.650 kb on + strand, within PFLU_RS01645 at 367.650 kb on + strand, within PFLU_RS01645 at 368.004 kb on + strand, within PFLU_RS01645 at 368.004 kb on + strand, within PFLU_RS01645 at 368.005 kb on - strand, within PFLU_RS01645 at 368.073 kb on - strand, within PFLU_RS01645 at 368.073 kb on - strand, within PFLU_RS01645 at 368.073 kb on - strand, within PFLU_RS01645 at 368.073 kb on - strand, within PFLU_RS01645 at 368.085 kb on + strand, within PFLU_RS01645 at 368.085 kb on + strand, within PFLU_RS01645 at 368.085 kb on + strand, within PFLU_RS01645 at 368.086 kb on - strand, within PFLU_RS01645 at 368.086 kb on - strand, within PFLU_RS01645 at 368.086 kb on - strand, within PFLU_RS01645 at 368.086 kb on - strand, within PFLU_RS01645 at 368.086 kb on - strand, within PFLU_RS01645 at 368.086 kb on - strand, within PFLU_RS01645 at 368.086 kb on - strand, within PFLU_RS01645 at 368.287 kb on + strand at 368.288 kb on - strand at 368.292 kb on + strand at 368.292 kb on + strand at 368.292 kb on + strand at 368.292 kb on + strand at 368.292 kb on + strand at 368.292 kb on + strand at 368.292 kb on + strand at 368.292 kb on + strand at 368.292 kb on + strand at 368.293 kb on - strand at 368.293 kb on - strand at 368.293 kb on - strand at 368.293 kb on - strand at 368.293 kb on - strand at 368.293 kb on - strand at 368.293 kb on - strand at 368.304 kb on + strand at 368.368 kb on - strand at 368.368 kb on - strand at 368.398 kb on + strand at 368.399 kb on - strand at 368.429 kb on + strand at 368.429 kb on + strand at 368.429 kb on + strand at 368.429 kb on + strand at 368.430 kb on - strand at 368.454 kb on - strand, within PFLU_RS01650 at 368.454 kb on - strand, within PFLU_RS01650 at 368.454 kb on - strand, within PFLU_RS01650 at 368.484 kb on + strand, within PFLU_RS01650 at 368.484 kb on + strand, within PFLU_RS01650 at 368.484 kb on + strand, within PFLU_RS01650 at 368.484 kb on + strand, within PFLU_RS01650 at 368.484 kb on + strand, within PFLU_RS01650 at 368.485 kb on - strand, within PFLU_RS01650 at 368.485 kb on - strand, within PFLU_RS01650 at 368.485 kb on - strand, within PFLU_RS01650 at 368.657 kb on + strand, within PFLU_RS01650 at 368.768 kb on - strand, within PFLU_RS01650 at 368.975 kb on + strand, within PFLU_RS01650 at 368.975 kb on + strand, within PFLU_RS01650 at 368.976 kb on - strand, within PFLU_RS01650 at 368.976 kb on - strand, within PFLU_RS01650 at 369.171 kb on + strand, within PFLU_RS01650 at 369.172 kb on - strand, within PFLU_RS01650 at 369.172 kb on - strand, within PFLU_RS01650 at 369.172 kb on - strand, within PFLU_RS01650 at 369.177 kb on + strand, within PFLU_RS01650 at 369.177 kb on + strand, within PFLU_RS01650 at 369.177 kb on + strand, within PFLU_RS01650 at 369.177 kb on + strand, within PFLU_RS01650 at 369.177 kb on + strand, within PFLU_RS01650 at 369.177 kb on + strand, within PFLU_RS01650 at 369.178 kb on - strand, within PFLU_RS01650 at 369.178 kb on - strand, within PFLU_RS01650 at 369.180 kb on + strand, within PFLU_RS01650 at 369.180 kb on + strand, within PFLU_RS01650 at 369.180 kb on + strand, within PFLU_RS01650 at 369.180 kb on + strand, within PFLU_RS01650 at 369.181 kb on - strand, within PFLU_RS01650 at 369.181 kb on - strand, within PFLU_RS01650 at 369.181 kb on - strand, within PFLU_RS01650 at 369.237 kb on + strand, within PFLU_RS01650 at 369.238 kb on - strand, within PFLU_RS01650 at 369.238 kb on - strand, within PFLU_RS01650 at 369.259 kb on - strand, within PFLU_RS01650
Per-strain Table
Position Strand Gene LocusTag Fraction NAG (C); with MOPS remove 365,957 + -0.7 365,957 + -1.6 365,957 + -0.6 365,957 + +0.3 365,957 + +0.1 365,958 - +1.1 365,958 - -1.1 365,958 - +1.2 365,958 - +0.5 365,958 - +0.8 365,958 - +0.5 365,958 - -0.7 365,958 - +0.0 365,958 - +0.4 365,958 - +0.2 365,958 - -0.4 365,958 - +1.2 365,958 - +0.6 365,958 - -2.1 366,039 + -0.8 366,039 + -1.2 366,040 - -1.7 366,040 - +1.1 366,043 + -0.0 366,043 + +0.3 366,043 + +0.6 366,043 + +1.2 366,043 + +1.2 366,043 + +0.3 366,043 + +0.8 366,043 + +2.6 366,043 + +1.0 366,044 - +0.2 366,044 - -1.5 366,044 - +0.6 366,044 - +1.0 366,044 - -2.6 366,134 + -0.4 366,134 + -1.3 366,135 - +0.5 366,135 - -1.6 366,164 - +0.1 366,164 - +0.6 366,167 + -0.9 366,167 + +1.4 366,167 + -0.0 366,167 + +0.0 366,167 + -0.1 366,167 + +1.3 366,167 + -3.5 366,167 + +0.4 366,167 + +0.4 366,167 + +0.1 366,167 + +1.8 366,167 + +0.3 366,167 + -0.7 366,167 + -1.0 366,168 - -0.3 366,168 - -0.3 366,168 - -0.7 366,168 - +1.1 366,168 - +0.8 366,168 - +0.3 366,168 - +0.1 366,168 - -0.1 366,168 - -1.3 366,168 - +1.2 366,168 - -0.1 366,168 - -0.9 366,168 - +0.7 366,168 - -1.9 366,168 - -1.5 366,171 + -1.1 366,189 - -0.7 366,189 - -0.4 366,253 - PFLU_RS01640 0.11 -1.2 366,393 + PFLU_RS01640 0.29 +0.9 366,607 - PFLU_RS01640 0.56 +1.4 366,608 + PFLU_RS01640 0.56 +0.6 366,608 + PFLU_RS01640 0.56 -1.5 366,608 + PFLU_RS01640 0.56 -1.9 366,955 - -0.9 366,955 - +0.5 367,014 + -4.5 367,119 + PFLU_RS01645 0.12 -3.3 367,119 + PFLU_RS01645 0.12 -3.5 367,119 + PFLU_RS01645 0.12 -4.4 367,119 + PFLU_RS01645 0.12 -3.3 367,119 + PFLU_RS01645 0.12 -4.6 367,119 + PFLU_RS01645 0.12 -4.1 367,119 + PFLU_RS01645 0.12 -3.9 367,119 + PFLU_RS01645 0.12 -4.2 367,119 + PFLU_RS01645 0.12 -3.8 367,120 - PFLU_RS01645 0.12 -3.7 367,120 - PFLU_RS01645 0.12 -3.0 367,122 + PFLU_RS01645 0.13 -0.8 367,122 + PFLU_RS01645 0.13 -3.6 367,122 + PFLU_RS01645 0.13 -3.9 367,122 + PFLU_RS01645 0.13 -3.6 367,215 + PFLU_RS01645 0.20 -2.6 367,215 + PFLU_RS01645 0.20 -1.3 367,215 + PFLU_RS01645 0.20 -2.8 367,216 - PFLU_RS01645 0.20 -2.9 367,216 - PFLU_RS01645 0.20 -4.5 367,216 - PFLU_RS01645 0.20 -3.1 367,216 - PFLU_RS01645 0.20 -4.4 367,218 + PFLU_RS01645 0.20 -3.9 367,218 + PFLU_RS01645 0.20 -4.3 367,218 + PFLU_RS01645 0.20 -2.9 367,218 + PFLU_RS01645 0.20 -3.6 367,219 - PFLU_RS01645 0.20 -4.3 367,219 - PFLU_RS01645 0.20 -3.8 367,464 + PFLU_RS01645 0.39 -3.8 367,465 - PFLU_RS01645 0.39 -4.0 367,537 + PFLU_RS01645 0.44 -3.6 367,650 + PFLU_RS01645 0.53 -3.0 367,650 + PFLU_RS01645 0.53 -2.8 367,650 + PFLU_RS01645 0.53 -2.6 368,004 + PFLU_RS01645 0.80 -3.0 368,004 + PFLU_RS01645 0.80 -3.7 368,005 - PFLU_RS01645 0.80 -3.4 368,073 - PFLU_RS01645 0.85 -2.5 368,073 - PFLU_RS01645 0.85 -4.5 368,073 - PFLU_RS01645 0.85 -3.1 368,073 - PFLU_RS01645 0.85 -3.7 368,085 + PFLU_RS01645 0.86 -3.9 368,085 + PFLU_RS01645 0.86 -2.7 368,085 + PFLU_RS01645 0.86 -3.2 368,086 - PFLU_RS01645 0.86 -3.8 368,086 - PFLU_RS01645 0.86 -3.5 368,086 - PFLU_RS01645 0.86 -1.7 368,086 - PFLU_RS01645 0.86 -1.7 368,086 - PFLU_RS01645 0.86 -4.5 368,086 - PFLU_RS01645 0.86 -3.2 368,086 - PFLU_RS01645 0.86 -2.2 368,287 + -3.1 368,288 - -1.9 368,292 + -4.4 368,292 + -3.4 368,292 + -4.4 368,292 + -2.7 368,292 + -1.5 368,292 + -3.0 368,292 + -4.6 368,292 + -3.5 368,292 + -2.3 368,293 - +0.3 368,293 - -1.0 368,293 - +0.1 368,293 - +0.4 368,293 - -0.9 368,293 - -2.2 368,293 - +1.1 368,304 + -1.9 368,368 - +0.1 368,368 - +0.8 368,398 + -2.3 368,399 - +3.4 368,429 + -0.0 368,429 + +0.3 368,429 + -0.7 368,429 + -0.2 368,430 - +1.2 368,454 - PFLU_RS01650 0.12 +1.3 368,454 - PFLU_RS01650 0.12 +0.1 368,454 - PFLU_RS01650 0.12 -1.3 368,484 + PFLU_RS01650 0.14 -1.8 368,484 + PFLU_RS01650 0.14 -1.3 368,484 + PFLU_RS01650 0.14 -1.5 368,484 + PFLU_RS01650 0.14 +0.7 368,484 + PFLU_RS01650 0.14 -0.5 368,485 - PFLU_RS01650 0.14 +0.1 368,485 - PFLU_RS01650 0.14 +1.0 368,485 - PFLU_RS01650 0.14 -0.3 368,657 + PFLU_RS01650 0.27 -0.7 368,768 - PFLU_RS01650 0.36 +0.7 368,975 + PFLU_RS01650 0.52 -2.2 368,975 + PFLU_RS01650 0.52 -1.3 368,976 - PFLU_RS01650 0.52 +1.8 368,976 - PFLU_RS01650 0.52 +0.5 369,171 + PFLU_RS01650 0.67 -0.8 369,172 - PFLU_RS01650 0.67 +0.7 369,172 - PFLU_RS01650 0.67 -0.1 369,172 - PFLU_RS01650 0.67 +0.8 369,177 + PFLU_RS01650 0.67 -1.4 369,177 + PFLU_RS01650 0.67 -2.3 369,177 + PFLU_RS01650 0.67 -3.3 369,177 + PFLU_RS01650 0.67 -3.9 369,177 + PFLU_RS01650 0.67 -1.4 369,177 + PFLU_RS01650 0.67 -1.9 369,178 - PFLU_RS01650 0.68 +0.5 369,178 - PFLU_RS01650 0.68 +0.3 369,180 + PFLU_RS01650 0.68 -4.9 369,180 + PFLU_RS01650 0.68 -1.1 369,180 + PFLU_RS01650 0.68 -3.8 369,180 + PFLU_RS01650 0.68 -1.0 369,181 - PFLU_RS01650 0.68 -1.3 369,181 - PFLU_RS01650 0.68 +1.1 369,181 - PFLU_RS01650 0.68 +0.5 369,237 + PFLU_RS01650 0.72 -3.1 369,238 - PFLU_RS01650 0.72 -1.0 369,238 - PFLU_RS01650 0.72 +0.5 369,259 - PFLU_RS01650 0.74 +0.4
Or see this region's nucleotide sequence