Strain Fitness in Pseudomonas fluorescens SBW25 around PFLU_RS20250

Experiment: NAG (C); with MOPS

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	NAG (C); with MOPS
4,580,626	+				-2.3
4,580,894	-		PFLU_RS31310	0.45	+0.6
4,581,008	-				+1.0
4,581,020	+				-1.6
4,581,020	+				+0.8
4,581,021	-				+0.2
4,581,021	-				+0.4
4,581,039	+				-1.7
4,581,039	+				+0.3
4,581,039	+				-2.0
4,581,039	+				-1.0
4,581,039	+				+0.2
4,581,039	+				-1.2
4,581,039	+				-0.7
4,581,039	+				-0.0
4,581,039	+				+1.4
4,581,040	-				+0.8
4,581,040	-				+0.1
4,581,040	-				+1.2
4,581,040	-				-1.5
4,581,040	-				+0.7
4,581,040	-				-0.7
4,581,040	-				+0.1
4,581,040	-				-0.4
4,581,040	-				+0.8
4,581,040	-				-1.1
4,581,040	-				+0.2
4,581,040	-				-0.4
4,581,040	-				+0.1
4,581,113	+		PFLU_RS20245	0.17	-0.9
4,581,171	+		PFLU_RS20245	0.26	+0.2
4,581,171	+		PFLU_RS20245	0.26	+0.4
4,581,172	-		PFLU_RS20245	0.27	-1.8
4,581,172	-		PFLU_RS20245	0.27	-2.0
4,581,172	-		PFLU_RS20245	0.27	-0.3
4,581,172	-		PFLU_RS20245	0.27	-1.7
4,581,286	+		PFLU_RS20245	0.46	+0.7
4,581,286	+		PFLU_RS20245	0.46	-0.5
4,581,286	+		PFLU_RS20245	0.46	+0.3
4,581,286	+		PFLU_RS20245	0.46	+0.6
4,581,287	-		PFLU_RS20245	0.46	+1.0
4,581,293	+		PFLU_RS20245	0.47	+0.8
4,581,293	+		PFLU_RS20245	0.47	-0.3
4,581,293	+		PFLU_RS20245	0.47	+2.3
4,581,293	+		PFLU_RS20245	0.47	-1.7
4,581,293	+		PFLU_RS20245	0.47	-2.5
4,581,293	+		PFLU_RS20245	0.47	+1.4
4,581,294	-		PFLU_RS20245	0.47	+1.2
4,581,294	-		PFLU_RS20245	0.47	+0.3
4,581,294	-		PFLU_RS20245	0.47	-1.0
4,581,294	-		PFLU_RS20245	0.47	-1.5
4,581,294	-		PFLU_RS20245	0.47	-1.3
4,581,294	-		PFLU_RS20245	0.47	-2.1
4,581,461	-		PFLU_RS20245	0.75	+0.5
4,581,461	-		PFLU_RS20245	0.75	-0.0
4,581,527	-		PFLU_RS20245	0.86	-2.7
4,581,527	-		PFLU_RS20245	0.86	-0.5
4,581,600	+				-1.6
4,581,600	+				-1.9
4,581,600	+				-2.2
4,581,600	+				+1.6
4,581,600	+				-0.6
4,581,600	+				-1.8
4,581,601	-				-2.8
4,581,601	-				+0.5
4,581,601	-				-0.0
4,581,601	-				-0.0
4,581,601	-				-1.0
4,581,601	-				+1.0
4,581,618	-				+0.9
4,581,629	+				+0.6
4,581,629	+				-0.6
4,581,629	+				+0.2
4,581,629	+				+0.8
4,581,630	-				-1.6
4,581,630	-				+0.6
4,581,630	-				+0.7
4,581,630	-				+1.2
4,581,630	-				+1.0
4,581,710	+		PFLU_RS20250	0.16	+1.4
4,581,720	+		PFLU_RS20250	0.18	+0.4
4,581,720	+		PFLU_RS20250	0.18	-2.5
4,581,720	+		PFLU_RS20250	0.18	+0.6
4,581,720	+		PFLU_RS20250	0.18	+2.2
4,581,720	+		PFLU_RS20250	0.18	-1.1
4,581,721	-		PFLU_RS20250	0.18	+0.3
4,581,721	-		PFLU_RS20250	0.18	+0.3
4,581,801	+		PFLU_RS20250	0.30	+0.3
4,581,868	+		PFLU_RS20250	0.40	-0.2
4,581,868	+		PFLU_RS20250	0.40	+0.2
4,581,868	+		PFLU_RS20250	0.40	-0.0
4,581,868	+		PFLU_RS20250	0.40	+0.6
4,581,868	+		PFLU_RS20250	0.40	-0.7
4,581,868	+		PFLU_RS20250	0.40	+1.5
4,581,868	+		PFLU_RS20250	0.40	-0.5
4,581,869	-		PFLU_RS20250	0.40	+0.8
4,581,869	-		PFLU_RS20250	0.40	-1.2
4,581,869	-		PFLU_RS20250	0.40	-0.2
4,581,869	-		PFLU_RS20250	0.40	+1.4
4,581,869	-		PFLU_RS20250	0.40	-2.3
4,581,869	-		PFLU_RS20250	0.40	-0.2
4,581,869	-		PFLU_RS20250	0.40	+2.2
4,581,882	-		PFLU_RS20250	0.42	+0.1
4,581,904	+		PFLU_RS20250	0.46	+1.2
4,581,904	+		PFLU_RS20250	0.46	-0.3
4,581,904	+		PFLU_RS20250	0.46	-0.0
4,581,904	+		PFLU_RS20250	0.46	-1.9
4,581,904	+		PFLU_RS20250	0.46	+1.5
4,581,904	+		PFLU_RS20250	0.46	-2.0
4,581,904	+		PFLU_RS20250	0.46	-2.1
4,581,904	+		PFLU_RS20250	0.46	+0.3
4,581,905	-		PFLU_RS20250	0.46	+1.4
4,581,905	-		PFLU_RS20250	0.46	+1.0
4,581,905	-		PFLU_RS20250	0.46	+2.3
4,581,905	-		PFLU_RS20250	0.46	+0.8
4,581,905	-		PFLU_RS20250	0.46	-1.1
4,581,905	-		PFLU_RS20250	0.46	+0.8
4,581,905	-		PFLU_RS20250	0.46	-0.6
4,581,905	-		PFLU_RS20250	0.46	+0.8
4,581,905	-		PFLU_RS20250	0.46	-0.4
4,581,905	-		PFLU_RS20250	0.46	+0.3
4,581,905	-		PFLU_RS20250	0.46	-0.1
4,581,905	-		PFLU_RS20250	0.46	+2.0
4,581,940	-		PFLU_RS20250	0.51	-0.8
4,581,972	+		PFLU_RS20250	0.56	+0.1
4,581,973	-		PFLU_RS20250	0.56	-0.1
4,581,973	-		PFLU_RS20250	0.56	-1.6
4,581,997	+		PFLU_RS20250	0.60	+2.3
4,581,997	+		PFLU_RS20250	0.60	+0.6
4,581,997	+		PFLU_RS20250	0.60	+0.6
4,581,997	+		PFLU_RS20250	0.60	+1.8
4,581,997	+		PFLU_RS20250	0.60	+1.0
4,581,997	+		PFLU_RS20250	0.60	-0.1
4,581,997	+		PFLU_RS20250	0.60	-3.2
4,581,997	+		PFLU_RS20250	0.60	+1.3
4,581,998	-		PFLU_RS20250	0.60	+0.1
4,581,998	-		PFLU_RS20250	0.60	-2.7
4,581,998	-		PFLU_RS20250	0.60	-0.2
4,581,998	-		PFLU_RS20250	0.60	+1.8
4,581,998	-		PFLU_RS20250	0.60	+1.4
4,581,998	-		PFLU_RS20250	0.60	-0.2
4,581,998	-		PFLU_RS20250	0.60	-0.4
4,581,998	-		PFLU_RS20250	0.60	-0.2
4,581,998	-		PFLU_RS20250	0.60	+1.1
4,581,998	-		PFLU_RS20250	0.60	+0.2
4,582,054	+		PFLU_RS20250	0.68	-0.6
4,582,055	-		PFLU_RS20250	0.68	-1.8
4,582,055	-		PFLU_RS20250	0.68	-0.5
4,582,055	-		PFLU_RS20250	0.68	+0.8
4,582,055	-		PFLU_RS20250	0.68	+0.5
4,582,055	-		PFLU_RS20250	0.68	+1.0
4,582,175	+		PFLU_RS20250	0.87	+1.6
4,582,175	+		PFLU_RS20250	0.87	-1.5
4,582,176	-		PFLU_RS20250	0.87	-0.4
4,582,176	-		PFLU_RS20250	0.87	+0.6
4,582,176	-		PFLU_RS20250	0.87	-0.0
4,582,281	+				+0.8
4,582,282	-				-0.8
4,582,282	-				+0.7
4,582,282	-				+0.9
4,582,282	-				+0.4
4,582,342	+		PFLU_RS20255	0.31	+1.0
4,582,342	+		PFLU_RS20255	0.31	+0.1
4,582,415	+		PFLU_RS20255	0.65	+1.6
4,582,416	-		PFLU_RS20255	0.65	-0.5
4,582,416	-		PFLU_RS20255	0.65	+1.1
4,582,491	+				+1.6
4,582,491	+				-1.2
4,582,491	+				-2.7
4,582,491	+				-0.7
4,582,491	+				+2.2
4,582,492	-				+0.6
4,582,504	+				+0.2
4,582,504	+				-1.0
4,582,504	+				+0.4
4,582,504	+				-0.4
4,582,504	+				-0.6
4,582,504	+				+0.7
4,582,505	-				+0.6
4,582,505	-				+1.1
4,582,505	-				-0.7
4,582,505	-				+2.4
4,582,505	-				-0.3
4,582,505	-				-0.4
4,582,801	+		PFLU_RS20260	0.63	+0.2
4,582,801	+		PFLU_RS20260	0.63	-1.6
4,582,801	+		PFLU_RS20260	0.63	+1.6
4,582,802	-		PFLU_RS20260	0.63	+1.3
4,582,907	+		PFLU_RS20260	0.85	+0.2
4,583,034	+				-1.4
4,583,034	+				-1.6
4,583,034	+				-1.7
4,583,177	+				-1.4
4,583,177	+				+2.1
4,583,177	+				-1.7
4,583,177	+				-2.1
4,583,177	+				+0.6
4,583,177	+				+1.6
4,583,177	+				+1.6
4,583,177	+				+0.8
4,583,177	+				-3.6
4,583,178	-				-0.3
4,583,178	-				-0.1
4,583,178	-				-1.2
4,583,178	-				-0.8