Experiment: NAG (C); with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS01565 and PFLU_RS01570 are separated by 89 nucleotides PFLU_RS01570 and PFLU_RS01575 are separated by 149 nucleotides PFLU_RS01575 and PFLU_RS01580 are separated by 86 nucleotides PFLU_RS01580 and PFLU_RS01585 are separated by 103 nucleotides PFLU_RS01585 and PFLU_RS01590 overlap by 4 nucleotides
PFLU_RS01565: PFLU_RS01565 - membrane protein, at 351,879 to 353,471
_RS01565
PFLU_RS01570: PFLU_RS01570 - hypothetical protein, at 353,561 to 353,836
_RS01570
PFLU_RS01575: PFLU_RS01575 - hypothetical protein, at 353,986 to 354,543
_RS01575
PFLU_RS01580: PFLU_RS01580 - tRNA-Phe, at 354,630 to 354,705
_RS01580
PFLU_RS01585: PFLU_RS01585 - oxidative damage protection protein, at 354,809 to 355,081
_RS01585
PFLU_RS01590: PFLU_RS01590 - A/G-specific adenine glycosylase, at 355,078 to 356,145
_RS01590
Position (kb) 353
354
355 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 352.992 kb on + strand, within PFLU_RS01565 at 352.993 kb on - strand, within PFLU_RS01565 at 353.005 kb on + strand, within PFLU_RS01565 at 353.006 kb on - strand, within PFLU_RS01565 at 353.006 kb on - strand, within PFLU_RS01565 at 353.006 kb on - strand, within PFLU_RS01565 at 353.006 kb on - strand, within PFLU_RS01565 at 353.006 kb on - strand, within PFLU_RS01565 at 353.006 kb on - strand, within PFLU_RS01565 at 353.087 kb on - strand, within PFLU_RS01565 at 353.191 kb on + strand, within PFLU_RS01565 at 353.231 kb on + strand, within PFLU_RS01565 at 353.231 kb on + strand, within PFLU_RS01565 at 353.231 kb on + strand, within PFLU_RS01565 at 353.232 kb on - strand, within PFLU_RS01565 at 353.265 kb on + strand, within PFLU_RS01565 at 353.265 kb on + strand, within PFLU_RS01565 at 353.266 kb on - strand, within PFLU_RS01565 at 353.266 kb on - strand, within PFLU_RS01565 at 353.266 kb on - strand, within PFLU_RS01565 at 353.266 kb on - strand, within PFLU_RS01565 at 353.266 kb on - strand, within PFLU_RS01565 at 353.313 kb on - strand at 353.313 kb on - strand at 353.359 kb on + strand at 353.487 kb on + strand at 353.487 kb on + strand at 353.487 kb on + strand at 353.488 kb on - strand at 353.488 kb on - strand at 353.488 kb on - strand at 353.549 kb on - strand at 353.567 kb on - strand at 353.567 kb on - strand at 353.567 kb on - strand at 353.584 kb on + strand at 353.585 kb on - strand at 353.812 kb on + strand at 353.812 kb on + strand at 353.812 kb on + strand at 353.812 kb on + strand at 353.812 kb on + strand at 353.812 kb on + strand at 353.813 kb on - strand at 353.813 kb on - strand at 353.813 kb on - strand at 353.813 kb on - strand at 353.813 kb on - strand at 353.900 kb on + strand at 353.900 kb on + strand at 353.901 kb on - strand at 353.939 kb on - strand at 353.939 kb on - strand at 353.958 kb on - strand at 353.958 kb on - strand at 354.029 kb on - strand at 354.040 kb on - strand at 354.048 kb on + strand, within PFLU_RS01575 at 354.049 kb on - strand, within PFLU_RS01575 at 354.063 kb on + strand, within PFLU_RS01575 at 354.063 kb on + strand, within PFLU_RS01575 at 354.063 kb on + strand, within PFLU_RS01575 at 354.063 kb on + strand, within PFLU_RS01575 at 354.064 kb on - strand, within PFLU_RS01575 at 354.064 kb on - strand, within PFLU_RS01575 at 354.064 kb on - strand, within PFLU_RS01575 at 354.064 kb on - strand, within PFLU_RS01575 at 354.064 kb on - strand, within PFLU_RS01575 at 354.064 kb on - strand, within PFLU_RS01575 at 354.064 kb on - strand, within PFLU_RS01575 at 354.148 kb on + strand, within PFLU_RS01575 at 354.148 kb on + strand, within PFLU_RS01575 at 354.149 kb on - strand, within PFLU_RS01575 at 354.149 kb on - strand, within PFLU_RS01575 at 354.149 kb on - strand, within PFLU_RS01575 at 354.149 kb on - strand, within PFLU_RS01575 at 354.206 kb on + strand, within PFLU_RS01575 at 354.206 kb on + strand, within PFLU_RS01575 at 354.206 kb on + strand, within PFLU_RS01575 at 354.206 kb on + strand, within PFLU_RS01575 at 354.207 kb on - strand, within PFLU_RS01575 at 354.312 kb on + strand, within PFLU_RS01575 at 354.312 kb on + strand, within PFLU_RS01575 at 354.313 kb on - strand, within PFLU_RS01575 at 354.328 kb on + strand, within PFLU_RS01575 at 354.344 kb on - strand, within PFLU_RS01575 at 354.397 kb on + strand, within PFLU_RS01575 at 354.397 kb on + strand, within PFLU_RS01575 at 354.397 kb on + strand, within PFLU_RS01575 at 354.398 kb on - strand, within PFLU_RS01575 at 354.398 kb on - strand, within PFLU_RS01575 at 354.398 kb on - strand, within PFLU_RS01575 at 354.398 kb on - strand, within PFLU_RS01575 at 354.400 kb on + strand, within PFLU_RS01575 at 354.400 kb on + strand, within PFLU_RS01575 at 354.400 kb on + strand, within PFLU_RS01575 at 354.400 kb on + strand, within PFLU_RS01575 at 354.400 kb on + strand, within PFLU_RS01575 at 354.401 kb on - strand, within PFLU_RS01575 at 354.401 kb on - strand, within PFLU_RS01575 at 354.401 kb on - strand, within PFLU_RS01575 at 354.401 kb on - strand, within PFLU_RS01575 at 354.401 kb on - strand, within PFLU_RS01575 at 354.401 kb on - strand, within PFLU_RS01575 at 354.401 kb on - strand, within PFLU_RS01575 at 354.401 kb on - strand, within PFLU_RS01575 at 354.401 kb on - strand, within PFLU_RS01575 at 354.429 kb on + strand, within PFLU_RS01575 at 354.429 kb on + strand, within PFLU_RS01575 at 354.430 kb on - strand, within PFLU_RS01575 at 354.450 kb on + strand, within PFLU_RS01575 at 354.486 kb on - strand, within PFLU_RS01575 at 354.503 kb on + strand at 354.503 kb on + strand at 354.503 kb on + strand at 354.503 kb on + strand at 354.504 kb on - strand at 354.515 kb on + strand at 354.516 kb on - strand at 354.541 kb on + strand at 354.766 kb on - strand at 354.769 kb on + strand at 354.770 kb on - strand at 354.782 kb on + strand at 354.829 kb on - strand at 354.829 kb on - strand at 354.829 kb on - strand at 354.846 kb on + strand, within PFLU_RS01585 at 354.846 kb on + strand, within PFLU_RS01585 at 354.888 kb on + strand, within PFLU_RS01585 at 354.889 kb on - strand, within PFLU_RS01585 at 354.889 kb on - strand, within PFLU_RS01585 at 354.889 kb on - strand, within PFLU_RS01585 at 354.889 kb on - strand, within PFLU_RS01585 at 354.892 kb on + strand, within PFLU_RS01585 at 354.892 kb on + strand, within PFLU_RS01585 at 354.892 kb on + strand, within PFLU_RS01585 at 354.892 kb on + strand, within PFLU_RS01585 at 354.892 kb on + strand, within PFLU_RS01585 at 354.892 kb on + strand, within PFLU_RS01585 at 354.892 kb on + strand, within PFLU_RS01585 at 354.893 kb on - strand, within PFLU_RS01585 at 354.893 kb on - strand, within PFLU_RS01585 at 354.893 kb on - strand, within PFLU_RS01585 at 354.893 kb on - strand, within PFLU_RS01585 at 354.893 kb on - strand, within PFLU_RS01585 at 354.893 kb on - strand, within PFLU_RS01585 at 355.053 kb on + strand, within PFLU_RS01585 at 355.053 kb on + strand, within PFLU_RS01585 at 355.053 kb on + strand, within PFLU_RS01585 at 355.053 kb on + strand, within PFLU_RS01585 at 355.054 kb on - strand at 355.054 kb on - strand at 355.054 kb on - strand at 355.066 kb on + strand at 355.067 kb on - strand at 355.067 kb on - strand at 355.102 kb on + strand at 355.102 kb on + strand at 355.102 kb on + strand at 355.102 kb on + strand at 355.102 kb on + strand at 355.102 kb on + strand at 355.103 kb on - strand at 355.103 kb on - strand at 355.103 kb on - strand at 355.173 kb on + strand at 355.173 kb on + strand at 355.174 kb on - strand at 355.174 kb on - strand at 355.175 kb on + strand at 355.175 kb on + strand at 355.175 kb on + strand at 355.175 kb on + strand at 355.176 kb on - strand at 355.176 kb on - strand at 355.176 kb on - strand at 355.176 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction NAG (C); with MOPS remove 352,992 + PFLU_RS01565 0.70 +0.5 352,993 - PFLU_RS01565 0.70 -0.7 353,005 + PFLU_RS01565 0.71 -0.2 353,006 - PFLU_RS01565 0.71 -2.2 353,006 - PFLU_RS01565 0.71 +1.3 353,006 - PFLU_RS01565 0.71 +0.5 353,006 - PFLU_RS01565 0.71 -1.6 353,006 - PFLU_RS01565 0.71 +1.0 353,006 - PFLU_RS01565 0.71 +0.5 353,087 - PFLU_RS01565 0.76 -1.0 353,191 + PFLU_RS01565 0.82 -0.6 353,231 + PFLU_RS01565 0.85 +1.2 353,231 + PFLU_RS01565 0.85 +2.3 353,231 + PFLU_RS01565 0.85 +0.9 353,232 - PFLU_RS01565 0.85 +1.9 353,265 + PFLU_RS01565 0.87 +0.4 353,265 + PFLU_RS01565 0.87 +0.8 353,266 - PFLU_RS01565 0.87 +0.8 353,266 - PFLU_RS01565 0.87 +1.2 353,266 - PFLU_RS01565 0.87 +0.4 353,266 - PFLU_RS01565 0.87 -0.5 353,266 - PFLU_RS01565 0.87 +1.7 353,313 - +1.1 353,313 - +1.0 353,359 + +0.7 353,487 + -3.5 353,487 + -0.8 353,487 + +0.6 353,488 - +0.7 353,488 - -0.3 353,488 - -2.2 353,549 - -0.6 353,567 - -0.7 353,567 - -0.5 353,567 - -0.4 353,584 + +0.8 353,585 - -0.6 353,812 + +0.9 353,812 + +1.2 353,812 + +1.0 353,812 + +2.3 353,812 + +0.6 353,812 + +0.9 353,813 - +1.2 353,813 - +0.8 353,813 - +0.2 353,813 - -0.7 353,813 - +0.4 353,900 + +0.3 353,900 + -0.5 353,901 - +0.9 353,939 - +0.5 353,939 - -0.9 353,958 - -1.3 353,958 - +1.7 354,029 - -1.7 354,040 - -0.9 354,048 + PFLU_RS01575 0.11 -0.3 354,049 - PFLU_RS01575 0.11 +1.5 354,063 + PFLU_RS01575 0.14 +0.0 354,063 + PFLU_RS01575 0.14 -0.0 354,063 + PFLU_RS01575 0.14 +0.9 354,063 + PFLU_RS01575 0.14 -0.0 354,064 - PFLU_RS01575 0.14 +1.3 354,064 - PFLU_RS01575 0.14 +0.6 354,064 - PFLU_RS01575 0.14 +0.4 354,064 - PFLU_RS01575 0.14 +1.3 354,064 - PFLU_RS01575 0.14 -0.3 354,064 - PFLU_RS01575 0.14 -1.0 354,064 - PFLU_RS01575 0.14 +0.3 354,148 + PFLU_RS01575 0.29 -0.0 354,148 + PFLU_RS01575 0.29 -0.1 354,149 - PFLU_RS01575 0.29 +0.1 354,149 - PFLU_RS01575 0.29 +0.2 354,149 - PFLU_RS01575 0.29 +1.1 354,149 - PFLU_RS01575 0.29 -0.3 354,206 + PFLU_RS01575 0.39 -1.2 354,206 + PFLU_RS01575 0.39 +1.1 354,206 + PFLU_RS01575 0.39 -1.0 354,206 + PFLU_RS01575 0.39 +0.4 354,207 - PFLU_RS01575 0.40 -0.8 354,312 + PFLU_RS01575 0.58 -0.8 354,312 + PFLU_RS01575 0.58 +1.7 354,313 - PFLU_RS01575 0.59 +0.5 354,328 + PFLU_RS01575 0.61 +1.1 354,344 - PFLU_RS01575 0.64 -0.2 354,397 + PFLU_RS01575 0.74 +1.0 354,397 + PFLU_RS01575 0.74 -0.3 354,397 + PFLU_RS01575 0.74 +1.9 354,398 - PFLU_RS01575 0.74 +0.3 354,398 - PFLU_RS01575 0.74 +0.0 354,398 - PFLU_RS01575 0.74 -0.1 354,398 - PFLU_RS01575 0.74 +0.5 354,400 + PFLU_RS01575 0.74 +0.5 354,400 + PFLU_RS01575 0.74 +1.7 354,400 + PFLU_RS01575 0.74 +0.8 354,400 + PFLU_RS01575 0.74 +1.2 354,400 + PFLU_RS01575 0.74 +0.5 354,401 - PFLU_RS01575 0.74 +0.8 354,401 - PFLU_RS01575 0.74 +0.6 354,401 - PFLU_RS01575 0.74 +1.1 354,401 - PFLU_RS01575 0.74 +0.6 354,401 - PFLU_RS01575 0.74 +0.0 354,401 - PFLU_RS01575 0.74 +0.6 354,401 - PFLU_RS01575 0.74 +0.4 354,401 - PFLU_RS01575 0.74 +0.5 354,401 - PFLU_RS01575 0.74 +0.5 354,429 + PFLU_RS01575 0.79 +0.5 354,429 + PFLU_RS01575 0.79 +0.3 354,430 - PFLU_RS01575 0.80 +0.0 354,450 + PFLU_RS01575 0.83 +0.5 354,486 - PFLU_RS01575 0.90 -0.8 354,503 + +0.2 354,503 + +0.2 354,503 + +0.5 354,503 + -0.1 354,504 - +0.4 354,515 + +0.3 354,516 - -2.3 354,541 + +1.8 354,766 - +0.8 354,769 + -0.1 354,770 - +0.9 354,782 + -1.7 354,829 - +0.5 354,829 - +0.7 354,829 - +0.3 354,846 + PFLU_RS01585 0.14 -1.8 354,846 + PFLU_RS01585 0.14 -1.9 354,888 + PFLU_RS01585 0.29 +1.0 354,889 - PFLU_RS01585 0.29 -1.3 354,889 - PFLU_RS01585 0.29 +2.2 354,889 - PFLU_RS01585 0.29 +0.9 354,889 - PFLU_RS01585 0.29 +0.2 354,892 + PFLU_RS01585 0.30 -0.2 354,892 + PFLU_RS01585 0.30 +1.1 354,892 + PFLU_RS01585 0.30 +0.7 354,892 + PFLU_RS01585 0.30 -0.1 354,892 + PFLU_RS01585 0.30 +0.9 354,892 + PFLU_RS01585 0.30 -0.5 354,892 + PFLU_RS01585 0.30 -2.4 354,893 - PFLU_RS01585 0.31 -0.9 354,893 - PFLU_RS01585 0.31 -0.6 354,893 - PFLU_RS01585 0.31 -0.6 354,893 - PFLU_RS01585 0.31 +0.8 354,893 - PFLU_RS01585 0.31 -0.4 354,893 - PFLU_RS01585 0.31 +1.1 355,053 + PFLU_RS01585 0.89 +1.5 355,053 + PFLU_RS01585 0.89 +1.4 355,053 + PFLU_RS01585 0.89 -2.7 355,053 + PFLU_RS01585 0.89 +0.3 355,054 - -1.3 355,054 - +1.0 355,054 - -1.0 355,066 + -1.2 355,067 - -0.3 355,067 - +2.1 355,102 + +0.9 355,102 + +0.2 355,102 + -0.7 355,102 + +0.3 355,102 + +0.5 355,102 + +1.5 355,103 - +0.2 355,103 - -0.6 355,103 - -0.7 355,173 + -0.7 355,173 + -2.3 355,174 - +0.3 355,174 - +1.2 355,175 + -0.6 355,175 + -0.4 355,175 + -1.0 355,175 + +0.2 355,176 - -1.4 355,176 - +0.5 355,176 - -2.0 355,176 - -0.6
Or see this region's nucleotide sequence