Experiment: KB with Acridine orange 3.125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4033 and Psyr_4034 are separated by 258 nucleotides Psyr_4034 and Psyr_4035 are separated by 41 nucleotides
Psyr_4033: Psyr_4033 - YD repeat protein, at 4,804,230 to 4,807,049
_4033
Psyr_4034: Psyr_4034 - hypothetical protein, at 4,807,308 to 4,810,253
_4034
Psyr_4035: Psyr_4035 - insecticidal toxin protein, putative, at 4,810,295 to 4,815,148
_4035
Position (kb) 4807
4808
4809
4810
4811 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 4806.349 kb on + strand, within Psyr_4033 at 4806.544 kb on + strand, within Psyr_4033 at 4806.631 kb on + strand, within Psyr_4033 at 4806.631 kb on + strand, within Psyr_4033 at 4806.632 kb on - strand, within Psyr_4033 at 4806.632 kb on - strand, within Psyr_4033 at 4806.632 kb on - strand, within Psyr_4033 at 4806.856 kb on + strand at 4806.856 kb on + strand at 4806.856 kb on + strand at 4806.857 kb on - strand at 4806.857 kb on - strand at 4806.919 kb on + strand at 4806.919 kb on + strand at 4807.025 kb on + strand at 4807.062 kb on + strand at 4807.073 kb on - strand at 4807.091 kb on + strand at 4807.170 kb on - strand at 4807.171 kb on + strand at 4807.171 kb on + strand at 4807.171 kb on + strand at 4807.204 kb on + strand at 4807.205 kb on - strand at 4807.253 kb on + strand at 4807.272 kb on - strand at 4807.334 kb on - strand at 4807.441 kb on + strand at 4807.481 kb on + strand at 4807.481 kb on + strand at 4807.481 kb on + strand at 4807.481 kb on + strand at 4807.481 kb on + strand at 4807.482 kb on - strand at 4807.482 kb on - strand at 4807.482 kb on - strand at 4807.622 kb on - strand, within Psyr_4034 at 4807.622 kb on - strand, within Psyr_4034 at 4807.690 kb on + strand, within Psyr_4034 at 4807.716 kb on + strand, within Psyr_4034 at 4807.717 kb on - strand, within Psyr_4034 at 4807.856 kb on + strand, within Psyr_4034 at 4807.856 kb on + strand, within Psyr_4034 at 4807.857 kb on - strand, within Psyr_4034 at 4807.882 kb on + strand, within Psyr_4034 at 4807.882 kb on + strand, within Psyr_4034 at 4807.882 kb on + strand, within Psyr_4034 at 4807.883 kb on - strand, within Psyr_4034 at 4807.883 kb on - strand, within Psyr_4034 at 4807.883 kb on - strand, within Psyr_4034 at 4807.883 kb on - strand, within Psyr_4034 at 4807.886 kb on + strand, within Psyr_4034 at 4807.886 kb on + strand, within Psyr_4034 at 4807.886 kb on + strand, within Psyr_4034 at 4807.886 kb on + strand, within Psyr_4034 at 4807.886 kb on + strand, within Psyr_4034 at 4807.886 kb on + strand, within Psyr_4034 at 4807.887 kb on - strand, within Psyr_4034 at 4807.887 kb on - strand, within Psyr_4034 at 4807.902 kb on + strand, within Psyr_4034 at 4807.902 kb on + strand, within Psyr_4034 at 4807.902 kb on + strand, within Psyr_4034 at 4807.902 kb on + strand, within Psyr_4034 at 4807.902 kb on + strand, within Psyr_4034 at 4807.937 kb on + strand, within Psyr_4034 at 4807.937 kb on + strand, within Psyr_4034 at 4807.937 kb on + strand, within Psyr_4034 at 4807.938 kb on - strand, within Psyr_4034 at 4807.938 kb on - strand, within Psyr_4034 at 4808.039 kb on - strand, within Psyr_4034 at 4808.052 kb on + strand, within Psyr_4034 at 4808.052 kb on + strand, within Psyr_4034 at 4808.053 kb on - strand, within Psyr_4034 at 4808.053 kb on - strand, within Psyr_4034 at 4808.253 kb on + strand, within Psyr_4034 at 4808.254 kb on - strand, within Psyr_4034 at 4808.254 kb on - strand, within Psyr_4034 at 4808.254 kb on - strand, within Psyr_4034 at 4808.254 kb on - strand, within Psyr_4034 at 4808.295 kb on - strand, within Psyr_4034 at 4808.376 kb on + strand, within Psyr_4034 at 4808.376 kb on + strand, within Psyr_4034 at 4808.376 kb on + strand, within Psyr_4034 at 4808.376 kb on + strand, within Psyr_4034 at 4808.376 kb on + strand, within Psyr_4034 at 4808.377 kb on - strand, within Psyr_4034 at 4808.476 kb on - strand, within Psyr_4034 at 4808.618 kb on + strand, within Psyr_4034 at 4808.693 kb on - strand, within Psyr_4034 at 4808.693 kb on - strand, within Psyr_4034 at 4808.696 kb on + strand, within Psyr_4034 at 4808.696 kb on + strand, within Psyr_4034 at 4808.697 kb on - strand, within Psyr_4034 at 4808.697 kb on - strand, within Psyr_4034 at 4808.697 kb on - strand, within Psyr_4034 at 4808.697 kb on - strand, within Psyr_4034 at 4808.697 kb on - strand, within Psyr_4034 at 4808.697 kb on - strand, within Psyr_4034 at 4808.697 kb on - strand, within Psyr_4034 at 4808.724 kb on + strand, within Psyr_4034 at 4809.272 kb on - strand, within Psyr_4034 at 4809.295 kb on + strand, within Psyr_4034 at 4809.356 kb on - strand, within Psyr_4034 at 4809.356 kb on - strand, within Psyr_4034 at 4809.432 kb on - strand, within Psyr_4034 at 4809.596 kb on - strand, within Psyr_4034 at 4809.685 kb on + strand, within Psyr_4034 at 4809.685 kb on + strand, within Psyr_4034 at 4809.686 kb on - strand, within Psyr_4034 at 4809.830 kb on - strand, within Psyr_4034 at 4809.830 kb on - strand, within Psyr_4034 at 4809.894 kb on + strand, within Psyr_4034 at 4809.895 kb on - strand, within Psyr_4034 at 4809.895 kb on - strand, within Psyr_4034 at 4809.895 kb on - strand, within Psyr_4034 at 4809.895 kb on - strand, within Psyr_4034 at 4809.960 kb on + strand at 4809.960 kb on + strand at 4810.143 kb on - strand at 4810.143 kb on - strand at 4810.168 kb on + strand at 4810.168 kb on + strand at 4810.168 kb on + strand at 4810.168 kb on + strand at 4810.168 kb on + strand at 4810.169 kb on - strand at 4810.169 kb on - strand at 4810.169 kb on - strand at 4810.169 kb on - strand at 4810.169 kb on - strand at 4810.222 kb on + strand at 4810.352 kb on + strand at 4810.352 kb on + strand at 4810.355 kb on + strand at 4810.355 kb on + strand at 4810.356 kb on - strand at 4810.356 kb on - strand at 4810.356 kb on - strand at 4810.356 kb on - strand at 4810.356 kb on - strand at 4810.384 kb on + strand at 4810.385 kb on - strand at 4810.487 kb on + strand at 4810.487 kb on + strand at 4810.487 kb on + strand at 4810.487 kb on + strand at 4810.487 kb on + strand at 4810.487 kb on + strand at 4810.487 kb on + strand at 4810.487 kb on + strand at 4810.487 kb on + strand at 4810.487 kb on + strand at 4810.488 kb on - strand at 4810.488 kb on - strand at 4810.488 kb on - strand at 4810.488 kb on - strand at 4810.488 kb on - strand at 4810.488 kb on - strand at 4810.488 kb on - strand at 4810.488 kb on - strand at 4810.488 kb on - strand at 4810.636 kb on + strand at 4810.664 kb on + strand at 4810.665 kb on - strand at 4810.665 kb on - strand at 4810.768 kb on - strand at 4810.775 kb on + strand at 4810.775 kb on + strand at 4810.823 kb on + strand, within Psyr_4035 at 4810.824 kb on - strand, within Psyr_4035 at 4810.824 kb on - strand, within Psyr_4035 at 4810.824 kb on - strand, within Psyr_4035 at 4810.856 kb on + strand, within Psyr_4035 at 4810.857 kb on - strand, within Psyr_4035 at 4810.857 kb on - strand, within Psyr_4035 at 4810.857 kb on - strand, within Psyr_4035 at 4810.859 kb on + strand, within Psyr_4035 at 4810.860 kb on - strand, within Psyr_4035 at 4810.860 kb on - strand, within Psyr_4035 at 4810.860 kb on - strand, within Psyr_4035 at 4810.889 kb on - strand, within Psyr_4035 at 4810.889 kb on - strand, within Psyr_4035 at 4810.892 kb on + strand, within Psyr_4035 at 4810.892 kb on + strand, within Psyr_4035 at 4810.892 kb on + strand, within Psyr_4035 at 4810.892 kb on + strand, within Psyr_4035 at 4810.892 kb on + strand, within Psyr_4035 at 4810.893 kb on - strand, within Psyr_4035 at 4810.896 kb on - strand, within Psyr_4035 at 4810.896 kb on - strand, within Psyr_4035 at 4810.896 kb on - strand, within Psyr_4035 at 4810.928 kb on + strand, within Psyr_4035 at 4811.020 kb on + strand, within Psyr_4035 at 4811.020 kb on + strand, within Psyr_4035 at 4811.048 kb on + strand, within Psyr_4035 at 4811.049 kb on - strand, within Psyr_4035 at 4811.106 kb on + strand, within Psyr_4035 at 4811.128 kb on + strand, within Psyr_4035 at 4811.129 kb on - strand, within Psyr_4035 at 4811.129 kb on - strand, within Psyr_4035 at 4811.129 kb on - strand, within Psyr_4035 at 4811.155 kb on + strand, within Psyr_4035 at 4811.221 kb on - strand, within Psyr_4035 at 4811.227 kb on + strand, within Psyr_4035 at 4811.228 kb on - strand, within Psyr_4035
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 3.125 ug/ml remove 4,806,349 + Psyr_4033 0.75 +4.5 4,806,544 + Psyr_4033 0.82 -0.3 4,806,631 + Psyr_4033 0.85 +1.3 4,806,631 + Psyr_4033 0.85 -1.6 4,806,632 - Psyr_4033 0.85 +1.0 4,806,632 - Psyr_4033 0.85 +1.2 4,806,632 - Psyr_4033 0.85 -2.0 4,806,856 + -0.1 4,806,856 + +1.4 4,806,856 + +0.9 4,806,857 - -0.3 4,806,857 - +0.3 4,806,919 + +2.4 4,806,919 + -1.6 4,807,025 + -0.2 4,807,062 + -0.2 4,807,073 - +0.4 4,807,091 + -1.4 4,807,170 - +0.5 4,807,171 + -0.6 4,807,171 + +0.1 4,807,171 + -0.2 4,807,204 + +0.4 4,807,205 - +0.8 4,807,253 + +0.7 4,807,272 - +0.4 4,807,334 - -0.5 4,807,441 + -1.0 4,807,481 + +3.6 4,807,481 + +3.9 4,807,481 + -1.2 4,807,481 + +0.4 4,807,481 + +2.7 4,807,482 - -2.7 4,807,482 - -1.8 4,807,482 - +0.4 4,807,622 - Psyr_4034 0.11 +3.0 4,807,622 - Psyr_4034 0.11 +0.0 4,807,690 + Psyr_4034 0.13 -0.6 4,807,716 + Psyr_4034 0.14 -1.2 4,807,717 - Psyr_4034 0.14 +4.1 4,807,856 + Psyr_4034 0.19 +3.0 4,807,856 + Psyr_4034 0.19 -0.2 4,807,857 - Psyr_4034 0.19 -2.7 4,807,882 + Psyr_4034 0.19 +0.0 4,807,882 + Psyr_4034 0.19 +2.7 4,807,882 + Psyr_4034 0.19 +1.7 4,807,883 - Psyr_4034 0.20 +1.4 4,807,883 - Psyr_4034 0.20 +0.5 4,807,883 - Psyr_4034 0.20 -3.0 4,807,883 - Psyr_4034 0.20 +4.0 4,807,886 + Psyr_4034 0.20 -1.1 4,807,886 + Psyr_4034 0.20 +2.7 4,807,886 + Psyr_4034 0.20 -2.9 4,807,886 + Psyr_4034 0.20 +0.4 4,807,886 + Psyr_4034 0.20 +0.6 4,807,886 + Psyr_4034 0.20 -1.0 4,807,887 - Psyr_4034 0.20 +1.7 4,807,887 - Psyr_4034 0.20 -0.6 4,807,902 + Psyr_4034 0.20 -0.8 4,807,902 + Psyr_4034 0.20 +0.6 4,807,902 + Psyr_4034 0.20 -0.2 4,807,902 + Psyr_4034 0.20 +1.7 4,807,902 + Psyr_4034 0.20 +2.3 4,807,937 + Psyr_4034 0.21 -2.9 4,807,937 + Psyr_4034 0.21 +1.0 4,807,937 + Psyr_4034 0.21 -0.7 4,807,938 - Psyr_4034 0.21 +0.8 4,807,938 - Psyr_4034 0.21 +1.1 4,808,039 - Psyr_4034 0.25 +1.4 4,808,052 + Psyr_4034 0.25 +2.0 4,808,052 + Psyr_4034 0.25 +1.3 4,808,053 - Psyr_4034 0.25 -0.1 4,808,053 - Psyr_4034 0.25 -0.3 4,808,253 + Psyr_4034 0.32 -0.2 4,808,254 - Psyr_4034 0.32 +1.0 4,808,254 - Psyr_4034 0.32 +3.6 4,808,254 - Psyr_4034 0.32 -0.4 4,808,254 - Psyr_4034 0.32 -1.2 4,808,295 - Psyr_4034 0.34 +0.2 4,808,376 + Psyr_4034 0.36 -1.0 4,808,376 + Psyr_4034 0.36 -0.1 4,808,376 + Psyr_4034 0.36 +1.7 4,808,376 + Psyr_4034 0.36 -0.1 4,808,376 + Psyr_4034 0.36 -1.6 4,808,377 - Psyr_4034 0.36 +0.1 4,808,476 - Psyr_4034 0.40 -2.0 4,808,618 + Psyr_4034 0.44 -1.7 4,808,693 - Psyr_4034 0.47 -0.3 4,808,693 - Psyr_4034 0.47 +2.7 4,808,696 + Psyr_4034 0.47 -0.1 4,808,696 + Psyr_4034 0.47 +5.0 4,808,697 - Psyr_4034 0.47 +1.9 4,808,697 - Psyr_4034 0.47 +1.3 4,808,697 - Psyr_4034 0.47 -0.1 4,808,697 - Psyr_4034 0.47 +4.6 4,808,697 - Psyr_4034 0.47 +0.4 4,808,697 - Psyr_4034 0.47 -0.9 4,808,697 - Psyr_4034 0.47 +0.4 4,808,724 + Psyr_4034 0.48 +0.4 4,809,272 - Psyr_4034 0.67 +0.1 4,809,295 + Psyr_4034 0.67 -0.7 4,809,356 - Psyr_4034 0.70 +2.4 4,809,356 - Psyr_4034 0.70 +2.0 4,809,432 - Psyr_4034 0.72 +0.4 4,809,596 - Psyr_4034 0.78 -3.9 4,809,685 + Psyr_4034 0.81 +2.4 4,809,685 + Psyr_4034 0.81 +2.3 4,809,686 - Psyr_4034 0.81 -0.3 4,809,830 - Psyr_4034 0.86 +0.2 4,809,830 - Psyr_4034 0.86 +0.6 4,809,894 + Psyr_4034 0.88 +0.1 4,809,895 - Psyr_4034 0.88 +2.3 4,809,895 - Psyr_4034 0.88 +3.0 4,809,895 - Psyr_4034 0.88 -0.8 4,809,895 - Psyr_4034 0.88 -0.1 4,809,960 + -0.3 4,809,960 + -0.4 4,810,143 - -2.9 4,810,143 - -2.2 4,810,168 + -1.8 4,810,168 + +1.2 4,810,168 + +1.2 4,810,168 + -0.0 4,810,168 + -0.1 4,810,169 - -3.6 4,810,169 - +0.0 4,810,169 - -1.1 4,810,169 - +1.1 4,810,169 - -2.4 4,810,222 + +2.7 4,810,352 + -3.8 4,810,352 + +0.0 4,810,355 + +1.8 4,810,355 + +1.6 4,810,356 - -1.5 4,810,356 - +0.4 4,810,356 - -0.4 4,810,356 - +0.6 4,810,356 - +0.4 4,810,384 + +1.2 4,810,385 - +2.0 4,810,487 + -1.9 4,810,487 + +0.9 4,810,487 + +0.3 4,810,487 + +0.4 4,810,487 + +0.3 4,810,487 + -3.6 4,810,487 + +2.4 4,810,487 + -2.1 4,810,487 + +1.6 4,810,487 + -0.3 4,810,488 - +1.8 4,810,488 - +2.7 4,810,488 - +1.4 4,810,488 - -0.7 4,810,488 - +0.4 4,810,488 - -1.1 4,810,488 - -2.0 4,810,488 - -0.7 4,810,488 - -1.9 4,810,636 + -0.9 4,810,664 + +0.1 4,810,665 - -0.6 4,810,665 - -0.4 4,810,768 - +2.5 4,810,775 + -3.6 4,810,775 + +0.4 4,810,823 + Psyr_4035 0.11 -0.8 4,810,824 - Psyr_4035 0.11 -4.2 4,810,824 - Psyr_4035 0.11 -0.8 4,810,824 - Psyr_4035 0.11 +3.0 4,810,856 + Psyr_4035 0.12 +2.8 4,810,857 - Psyr_4035 0.12 -0.5 4,810,857 - Psyr_4035 0.12 +0.2 4,810,857 - Psyr_4035 0.12 -0.7 4,810,859 + Psyr_4035 0.12 +0.7 4,810,860 - Psyr_4035 0.12 +1.5 4,810,860 - Psyr_4035 0.12 +3.8 4,810,860 - Psyr_4035 0.12 -0.6 4,810,889 - Psyr_4035 0.12 +0.7 4,810,889 - Psyr_4035 0.12 -2.9 4,810,892 + Psyr_4035 0.12 +0.4 4,810,892 + Psyr_4035 0.12 +1.1 4,810,892 + Psyr_4035 0.12 +0.4 4,810,892 + Psyr_4035 0.12 -0.1 4,810,892 + Psyr_4035 0.12 -2.5 4,810,893 - Psyr_4035 0.12 +0.1 4,810,896 - Psyr_4035 0.12 -0.6 4,810,896 - Psyr_4035 0.12 -0.9 4,810,896 - Psyr_4035 0.12 -0.9 4,810,928 + Psyr_4035 0.13 -2.6 4,811,020 + Psyr_4035 0.15 +0.9 4,811,020 + Psyr_4035 0.15 -1.3 4,811,048 + Psyr_4035 0.16 -0.3 4,811,049 - Psyr_4035 0.16 +2.4 4,811,106 + Psyr_4035 0.17 -2.9 4,811,128 + Psyr_4035 0.17 +0.6 4,811,129 - Psyr_4035 0.17 -0.4 4,811,129 - Psyr_4035 0.17 +0.8 4,811,129 - Psyr_4035 0.17 -2.9 4,811,155 + Psyr_4035 0.18 +0.4 4,811,221 - Psyr_4035 0.19 -0.8 4,811,227 + Psyr_4035 0.19 -1.5 4,811,228 - Psyr_4035 0.19 -1.4
Or see this region's nucleotide sequence