Experiment: KB with Acridine orange 3.125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3268 and Psyr_3269 are separated by 68 nucleotides Psyr_3269 and Psyr_3270 are separated by 42 nucleotides Psyr_3270 and Psyr_3271 are separated by 35 nucleotides
Psyr_3268: Psyr_3268 - myo-inositol 2-dehydrogenase, at 3,913,469 to 3,914,479
_3268
Psyr_3269: Psyr_3269 - 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase, at 3,914,548 to 3,916,485
_3269
Psyr_3270: Psyr_3270 - AP endonuclease, family 2, at 3,916,528 to 3,917,358
_3270
Psyr_3271: Psyr_3271 - methylmalonate-semialdehyde dehydrogenase (acylating), at 3,917,394 to 3,918,896
_3271
Position (kb)
3914
3915
3916
3917 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3913.608 kb on - strand, within Psyr_3268 at 3913.641 kb on + strand, within Psyr_3268 at 3913.782 kb on + strand, within Psyr_3268 at 3913.879 kb on + strand, within Psyr_3268 at 3913.879 kb on + strand, within Psyr_3268 at 3913.879 kb on + strand, within Psyr_3268 at 3913.914 kb on + strand, within Psyr_3268 at 3913.914 kb on + strand, within Psyr_3268 at 3913.914 kb on + strand, within Psyr_3268 at 3913.914 kb on + strand, within Psyr_3268 at 3913.914 kb on + strand, within Psyr_3268 at 3913.914 kb on + strand, within Psyr_3268 at 3913.915 kb on - strand, within Psyr_3268 at 3913.915 kb on - strand, within Psyr_3268 at 3913.915 kb on - strand, within Psyr_3268 at 3913.940 kb on + strand, within Psyr_3268 at 3913.940 kb on + strand, within Psyr_3268 at 3913.957 kb on + strand, within Psyr_3268 at 3913.990 kb on - strand, within Psyr_3268 at 3913.990 kb on - strand, within Psyr_3268 at 3913.990 kb on - strand, within Psyr_3268 at 3913.990 kb on - strand, within Psyr_3268 at 3914.155 kb on + strand, within Psyr_3268 at 3914.155 kb on + strand, within Psyr_3268 at 3914.156 kb on - strand, within Psyr_3268 at 3914.201 kb on + strand, within Psyr_3268 at 3914.235 kb on + strand, within Psyr_3268 at 3914.235 kb on + strand, within Psyr_3268 at 3914.235 kb on + strand, within Psyr_3268 at 3914.235 kb on + strand, within Psyr_3268 at 3914.236 kb on - strand, within Psyr_3268 at 3914.236 kb on - strand, within Psyr_3268 at 3914.236 kb on - strand, within Psyr_3268 at 3914.236 kb on - strand, within Psyr_3268 at 3914.236 kb on - strand, within Psyr_3268 at 3914.316 kb on + strand, within Psyr_3268 at 3914.317 kb on - strand, within Psyr_3268 at 3914.553 kb on - strand at 3914.591 kb on + strand at 3914.654 kb on + strand at 3914.654 kb on + strand at 3914.654 kb on + strand at 3914.654 kb on + strand at 3914.654 kb on + strand at 3914.655 kb on - strand at 3914.655 kb on - strand at 3914.655 kb on - strand at 3914.765 kb on + strand, within Psyr_3269 at 3914.765 kb on + strand, within Psyr_3269 at 3914.765 kb on + strand, within Psyr_3269 at 3914.765 kb on + strand, within Psyr_3269 at 3914.766 kb on - strand, within Psyr_3269 at 3914.766 kb on - strand, within Psyr_3269 at 3914.766 kb on - strand, within Psyr_3269 at 3914.766 kb on - strand, within Psyr_3269 at 3914.922 kb on + strand, within Psyr_3269 at 3914.923 kb on - strand, within Psyr_3269 at 3914.923 kb on - strand, within Psyr_3269 at 3914.923 kb on - strand, within Psyr_3269 at 3914.923 kb on - strand, within Psyr_3269 at 3914.996 kb on + strand, within Psyr_3269 at 3914.996 kb on + strand, within Psyr_3269 at 3914.996 kb on + strand, within Psyr_3269 at 3914.996 kb on + strand, within Psyr_3269 at 3914.996 kb on + strand, within Psyr_3269 at 3914.996 kb on + strand, within Psyr_3269 at 3914.996 kb on + strand, within Psyr_3269 at 3914.996 kb on + strand, within Psyr_3269 at 3914.996 kb on + strand, within Psyr_3269 at 3914.997 kb on - strand, within Psyr_3269 at 3914.997 kb on - strand, within Psyr_3269 at 3914.997 kb on - strand, within Psyr_3269 at 3914.997 kb on - strand, within Psyr_3269 at 3915.107 kb on + strand, within Psyr_3269 at 3915.107 kb on + strand, within Psyr_3269 at 3915.107 kb on + strand, within Psyr_3269 at 3915.108 kb on - strand, within Psyr_3269 at 3915.111 kb on + strand, within Psyr_3269 at 3915.112 kb on - strand, within Psyr_3269 at 3915.112 kb on - strand, within Psyr_3269 at 3915.112 kb on - strand, within Psyr_3269 at 3915.112 kb on - strand, within Psyr_3269 at 3915.115 kb on + strand, within Psyr_3269 at 3915.115 kb on + strand, within Psyr_3269 at 3915.115 kb on + strand, within Psyr_3269 at 3915.116 kb on - strand, within Psyr_3269 at 3915.332 kb on + strand, within Psyr_3269 at 3915.811 kb on + strand, within Psyr_3269 at 3915.811 kb on + strand, within Psyr_3269 at 3915.811 kb on + strand, within Psyr_3269 at 3915.811 kb on + strand, within Psyr_3269 at 3915.812 kb on - strand, within Psyr_3269 at 3915.812 kb on - strand, within Psyr_3269 at 3915.812 kb on - strand, within Psyr_3269 at 3915.812 kb on - strand, within Psyr_3269 at 3915.812 kb on - strand, within Psyr_3269 at 3915.812 kb on - strand, within Psyr_3269 at 3915.812 kb on - strand, within Psyr_3269 at 3915.812 kb on - strand, within Psyr_3269 at 3915.812 kb on - strand, within Psyr_3269 at 3915.862 kb on - strand, within Psyr_3269 at 3916.128 kb on - strand, within Psyr_3269 at 3916.232 kb on + strand, within Psyr_3269 at 3916.233 kb on - strand, within Psyr_3269 at 3916.233 kb on - strand, within Psyr_3269 at 3916.233 kb on - strand, within Psyr_3269 at 3916.428 kb on - strand at 3916.428 kb on - strand at 3916.479 kb on + strand at 3916.479 kb on + strand at 3916.480 kb on - strand at 3916.770 kb on + strand, within Psyr_3270 at 3916.770 kb on + strand, within Psyr_3270 at 3916.770 kb on + strand, within Psyr_3270 at 3916.770 kb on + strand, within Psyr_3270 at 3916.770 kb on + strand, within Psyr_3270 at 3916.771 kb on - strand, within Psyr_3270 at 3916.771 kb on - strand, within Psyr_3270 at 3916.771 kb on - strand, within Psyr_3270 at 3916.771 kb on - strand, within Psyr_3270 at 3916.774 kb on + strand, within Psyr_3270 at 3916.775 kb on - strand, within Psyr_3270 at 3917.145 kb on - strand, within Psyr_3270 at 3917.396 kb on - strand at 3917.467 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 3.125 ug/ml remove 3,913,608 - Psyr_3268 0.14 -0.7 3,913,641 + Psyr_3268 0.17 -1.4 3,913,782 + Psyr_3268 0.31 -4.2 3,913,879 + Psyr_3268 0.41 +0.2 3,913,879 + Psyr_3268 0.41 -1.4 3,913,879 + Psyr_3268 0.41 +3.0 3,913,914 + Psyr_3268 0.44 -1.5 3,913,914 + Psyr_3268 0.44 +0.0 3,913,914 + Psyr_3268 0.44 -0.6 3,913,914 + Psyr_3268 0.44 +3.8 3,913,914 + Psyr_3268 0.44 -2.8 3,913,914 + Psyr_3268 0.44 +1.8 3,913,915 - Psyr_3268 0.44 +0.4 3,913,915 - Psyr_3268 0.44 +1.8 3,913,915 - Psyr_3268 0.44 -0.9 3,913,940 + Psyr_3268 0.47 +0.2 3,913,940 + Psyr_3268 0.47 +0.9 3,913,957 + Psyr_3268 0.48 -0.3 3,913,990 - Psyr_3268 0.52 -2.2 3,913,990 - Psyr_3268 0.52 -2.1 3,913,990 - Psyr_3268 0.52 -1.8 3,913,990 - Psyr_3268 0.52 +2.2 3,914,155 + Psyr_3268 0.68 +0.3 3,914,155 + Psyr_3268 0.68 -2.3 3,914,156 - Psyr_3268 0.68 -0.5 3,914,201 + Psyr_3268 0.72 +0.0 3,914,235 + Psyr_3268 0.76 -0.6 3,914,235 + Psyr_3268 0.76 +0.2 3,914,235 + Psyr_3268 0.76 +2.8 3,914,235 + Psyr_3268 0.76 +1.1 3,914,236 - Psyr_3268 0.76 +0.2 3,914,236 - Psyr_3268 0.76 +0.2 3,914,236 - Psyr_3268 0.76 +0.3 3,914,236 - Psyr_3268 0.76 -2.4 3,914,236 - Psyr_3268 0.76 -3.4 3,914,316 + Psyr_3268 0.84 +1.8 3,914,317 - Psyr_3268 0.84 -0.2 3,914,553 - -2.0 3,914,591 + +0.2 3,914,654 + +0.0 3,914,654 + -2.6 3,914,654 + +0.4 3,914,654 + -0.8 3,914,654 + -1.3 3,914,655 - +0.7 3,914,655 - -3.8 3,914,655 - +0.9 3,914,765 + Psyr_3269 0.11 -1.3 3,914,765 + Psyr_3269 0.11 -2.4 3,914,765 + Psyr_3269 0.11 +0.5 3,914,765 + Psyr_3269 0.11 +0.2 3,914,766 - Psyr_3269 0.11 -1.9 3,914,766 - Psyr_3269 0.11 -1.6 3,914,766 - Psyr_3269 0.11 -0.5 3,914,766 - Psyr_3269 0.11 +1.2 3,914,922 + Psyr_3269 0.19 -0.7 3,914,923 - Psyr_3269 0.19 -0.4 3,914,923 - Psyr_3269 0.19 -1.0 3,914,923 - Psyr_3269 0.19 -1.1 3,914,923 - Psyr_3269 0.19 -4.0 3,914,996 + Psyr_3269 0.23 -0.4 3,914,996 + Psyr_3269 0.23 -2.1 3,914,996 + Psyr_3269 0.23 -1.1 3,914,996 + Psyr_3269 0.23 -1.4 3,914,996 + Psyr_3269 0.23 +0.5 3,914,996 + Psyr_3269 0.23 +0.5 3,914,996 + Psyr_3269 0.23 +1.0 3,914,996 + Psyr_3269 0.23 -3.2 3,914,996 + Psyr_3269 0.23 -1.5 3,914,997 - Psyr_3269 0.23 +0.3 3,914,997 - Psyr_3269 0.23 -0.3 3,914,997 - Psyr_3269 0.23 -1.4 3,914,997 - Psyr_3269 0.23 -1.6 3,915,107 + Psyr_3269 0.29 +0.2 3,915,107 + Psyr_3269 0.29 +3.0 3,915,107 + Psyr_3269 0.29 -0.8 3,915,108 - Psyr_3269 0.29 +0.1 3,915,111 + Psyr_3269 0.29 +0.2 3,915,112 - Psyr_3269 0.29 +0.5 3,915,112 - Psyr_3269 0.29 -0.0 3,915,112 - Psyr_3269 0.29 -1.5 3,915,112 - Psyr_3269 0.29 -1.8 3,915,115 + Psyr_3269 0.29 -3.7 3,915,115 + Psyr_3269 0.29 -1.0 3,915,115 + Psyr_3269 0.29 -2.1 3,915,116 - Psyr_3269 0.29 -3.5 3,915,332 + Psyr_3269 0.40 -1.4 3,915,811 + Psyr_3269 0.65 +2.6 3,915,811 + Psyr_3269 0.65 +4.0 3,915,811 + Psyr_3269 0.65 +2.2 3,915,811 + Psyr_3269 0.65 -2.9 3,915,812 - Psyr_3269 0.65 -2.8 3,915,812 - Psyr_3269 0.65 -0.1 3,915,812 - Psyr_3269 0.65 -0.5 3,915,812 - Psyr_3269 0.65 -0.3 3,915,812 - Psyr_3269 0.65 +3.6 3,915,812 - Psyr_3269 0.65 +0.1 3,915,812 - Psyr_3269 0.65 +2.2 3,915,812 - Psyr_3269 0.65 -0.8 3,915,812 - Psyr_3269 0.65 +3.2 3,915,862 - Psyr_3269 0.68 +4.2 3,916,128 - Psyr_3269 0.82 +0.2 3,916,232 + Psyr_3269 0.87 -0.3 3,916,233 - Psyr_3269 0.87 +0.1 3,916,233 - Psyr_3269 0.87 -2.0 3,916,233 - Psyr_3269 0.87 +3.2 3,916,428 - -0.3 3,916,428 - -0.8 3,916,479 + +0.7 3,916,479 + +0.2 3,916,480 - +0.4 3,916,770 + Psyr_3270 0.29 -0.4 3,916,770 + Psyr_3270 0.29 -1.6 3,916,770 + Psyr_3270 0.29 +1.7 3,916,770 + Psyr_3270 0.29 -0.3 3,916,770 + Psyr_3270 0.29 +2.2 3,916,771 - Psyr_3270 0.29 -0.3 3,916,771 - Psyr_3270 0.29 -3.8 3,916,771 - Psyr_3270 0.29 +0.6 3,916,771 - Psyr_3270 0.29 +2.2 3,916,774 + Psyr_3270 0.30 -0.0 3,916,775 - Psyr_3270 0.30 +0.5 3,917,145 - Psyr_3270 0.74 -3.3 3,917,396 - -2.8 3,917,467 + -3.3
Or see this region's nucleotide sequence