Experiment: KB with Acridine orange 3.125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2993 and Psyr_2994 overlap by 11 nucleotides Psyr_2994 and Psyr_2995 overlap by 4 nucleotides
Psyr_2993: Psyr_2993 - maltooligosyl trehalose hydrolase, at 3,585,283 to 3,587,097
_2993
Psyr_2994: Psyr_2994 - Glycoside hydrolase, family 77, at 3,587,087 to 3,589,165
_2994
Psyr_2995: Psyr_2995 - maltooligosyl trehalose synthase, at 3,589,162 to 3,591,945
_2995
Position (kb)
3587
3588
3589
3590 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3586.264 kb on + strand, within Psyr_2993 at 3586.264 kb on + strand, within Psyr_2993 at 3586.265 kb on - strand, within Psyr_2993 at 3586.265 kb on - strand, within Psyr_2993 at 3586.330 kb on + strand, within Psyr_2993 at 3586.360 kb on - strand, within Psyr_2993 at 3586.754 kb on + strand, within Psyr_2993 at 3586.754 kb on + strand, within Psyr_2993 at 3586.754 kb on + strand, within Psyr_2993 at 3586.754 kb on + strand, within Psyr_2993 at 3586.754 kb on + strand, within Psyr_2993 at 3586.754 kb on + strand, within Psyr_2993 at 3586.754 kb on + strand, within Psyr_2993 at 3586.754 kb on + strand, within Psyr_2993 at 3586.754 kb on + strand, within Psyr_2993 at 3586.754 kb on + strand, within Psyr_2993 at 3586.755 kb on - strand, within Psyr_2993 at 3586.755 kb on - strand, within Psyr_2993 at 3586.755 kb on - strand, within Psyr_2993 at 3586.755 kb on - strand, within Psyr_2993 at 3586.755 kb on - strand, within Psyr_2993 at 3586.786 kb on + strand, within Psyr_2993 at 3586.786 kb on + strand, within Psyr_2993 at 3586.787 kb on - strand, within Psyr_2993 at 3586.787 kb on - strand, within Psyr_2993 at 3586.861 kb on - strand, within Psyr_2993 at 3586.982 kb on + strand at 3586.983 kb on - strand at 3587.039 kb on - strand at 3587.039 kb on - strand at 3587.365 kb on - strand, within Psyr_2994 at 3587.365 kb on - strand, within Psyr_2994 at 3587.564 kb on + strand, within Psyr_2994 at 3587.715 kb on - strand, within Psyr_2994 at 3587.904 kb on - strand, within Psyr_2994 at 3588.510 kb on - strand, within Psyr_2994 at 3588.854 kb on + strand, within Psyr_2994 at 3588.855 kb on - strand, within Psyr_2994 at 3588.958 kb on + strand at 3588.959 kb on - strand at 3588.959 kb on - strand at 3588.959 kb on - strand at 3589.122 kb on + strand at 3589.122 kb on + strand at 3589.123 kb on - strand at 3589.175 kb on + strand at 3589.175 kb on + strand at 3589.176 kb on - strand at 3589.176 kb on - strand at 3589.253 kb on - strand at 3589.253 kb on - strand at 3589.253 kb on - strand at 3589.283 kb on - strand at 3589.344 kb on + strand at 3589.516 kb on + strand, within Psyr_2995 at 3589.517 kb on - strand, within Psyr_2995 at 3589.562 kb on + strand, within Psyr_2995 at 3589.601 kb on - strand, within Psyr_2995 at 3589.663 kb on + strand, within Psyr_2995 at 3589.664 kb on - strand, within Psyr_2995 at 3589.664 kb on - strand, within Psyr_2995 at 3589.664 kb on - strand, within Psyr_2995 at 3589.676 kb on - strand, within Psyr_2995 at 3589.708 kb on + strand, within Psyr_2995 at 3589.708 kb on + strand, within Psyr_2995 at 3589.795 kb on + strand, within Psyr_2995 at 3589.795 kb on + strand, within Psyr_2995 at 3589.796 kb on - strand, within Psyr_2995
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 3.125 ug/ml remove 3,586,264 + Psyr_2993 0.54 +0.2 3,586,264 + Psyr_2993 0.54 -3.7 3,586,265 - Psyr_2993 0.54 -1.1 3,586,265 - Psyr_2993 0.54 +0.2 3,586,330 + Psyr_2993 0.58 +0.7 3,586,360 - Psyr_2993 0.59 +0.2 3,586,754 + Psyr_2993 0.81 +3.5 3,586,754 + Psyr_2993 0.81 +2.5 3,586,754 + Psyr_2993 0.81 -2.3 3,586,754 + Psyr_2993 0.81 +3.9 3,586,754 + Psyr_2993 0.81 -0.9 3,586,754 + Psyr_2993 0.81 -0.5 3,586,754 + Psyr_2993 0.81 +0.9 3,586,754 + Psyr_2993 0.81 -1.0 3,586,754 + Psyr_2993 0.81 -0.4 3,586,754 + Psyr_2993 0.81 -2.1 3,586,755 - Psyr_2993 0.81 +0.2 3,586,755 - Psyr_2993 0.81 +0.1 3,586,755 - Psyr_2993 0.81 +3.7 3,586,755 - Psyr_2993 0.81 -0.5 3,586,755 - Psyr_2993 0.81 -0.6 3,586,786 + Psyr_2993 0.83 -0.5 3,586,786 + Psyr_2993 0.83 +2.8 3,586,787 - Psyr_2993 0.83 -0.6 3,586,787 - Psyr_2993 0.83 +1.7 3,586,861 - Psyr_2993 0.87 -0.2 3,586,982 + -0.6 3,586,983 - +0.8 3,587,039 - +3.0 3,587,039 - -1.8 3,587,365 - Psyr_2994 0.13 +3.2 3,587,365 - Psyr_2994 0.13 -2.0 3,587,564 + Psyr_2994 0.23 -2.8 3,587,715 - Psyr_2994 0.30 +0.7 3,587,904 - Psyr_2994 0.39 -2.0 3,588,510 - Psyr_2994 0.68 -0.2 3,588,854 + Psyr_2994 0.85 +0.9 3,588,855 - Psyr_2994 0.85 -0.6 3,588,958 + -1.4 3,588,959 - +1.8 3,588,959 - -1.1 3,588,959 - +1.8 3,589,122 + -2.5 3,589,122 + -3.0 3,589,123 - -3.6 3,589,175 + +0.2 3,589,175 + +0.2 3,589,176 - +0.8 3,589,176 - +0.2 3,589,253 - +3.4 3,589,253 - +1.4 3,589,253 - +0.2 3,589,283 - -2.2 3,589,344 + -2.4 3,589,516 + Psyr_2995 0.13 -0.9 3,589,517 - Psyr_2995 0.13 -2.1 3,589,562 + Psyr_2995 0.14 +3.7 3,589,601 - Psyr_2995 0.16 +3.9 3,589,663 + Psyr_2995 0.18 -1.2 3,589,664 - Psyr_2995 0.18 +2.5 3,589,664 - Psyr_2995 0.18 -3.1 3,589,664 - Psyr_2995 0.18 -1.4 3,589,676 - Psyr_2995 0.18 -1.2 3,589,708 + Psyr_2995 0.20 -0.7 3,589,708 + Psyr_2995 0.20 -0.6 3,589,795 + Psyr_2995 0.23 -4.2 3,589,795 + Psyr_2995 0.23 +4.5 3,589,796 - Psyr_2995 0.23 +1.4
Or see this region's nucleotide sequence