Experiment: KB with Acridine orange 3.125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2992 and Psyr_2993 are separated by 16 nucleotides Psyr_2993 and Psyr_2994 overlap by 11 nucleotides
Psyr_2992: Psyr_2992 - Starch (bacterial glycogen) synthase, at 3,583,668 to 3,585,266
_2992
Psyr_2993: Psyr_2993 - maltooligosyl trehalose hydrolase, at 3,585,283 to 3,587,097
_2993
Psyr_2994: Psyr_2994 - Glycoside hydrolase, family 77, at 3,587,087 to 3,589,165
_2994
Position (kb)
3585
3586
3587
3588 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3584.324 kb on - strand, within Psyr_2992 at 3584.324 kb on - strand, within Psyr_2992 at 3584.328 kb on + strand, within Psyr_2992 at 3584.329 kb on - strand, within Psyr_2992 at 3584.387 kb on + strand, within Psyr_2992 at 3584.409 kb on + strand, within Psyr_2992 at 3584.478 kb on + strand, within Psyr_2992 at 3584.478 kb on + strand, within Psyr_2992 at 3584.479 kb on - strand, within Psyr_2992 at 3584.667 kb on + strand, within Psyr_2992 at 3584.667 kb on + strand, within Psyr_2992 at 3584.667 kb on + strand, within Psyr_2992 at 3584.667 kb on + strand, within Psyr_2992 at 3584.668 kb on - strand, within Psyr_2992 at 3584.668 kb on - strand, within Psyr_2992 at 3584.668 kb on - strand, within Psyr_2992 at 3584.982 kb on + strand, within Psyr_2992 at 3584.982 kb on + strand, within Psyr_2992 at 3584.982 kb on + strand, within Psyr_2992 at 3584.983 kb on - strand, within Psyr_2992 at 3584.983 kb on - strand, within Psyr_2992 at 3585.020 kb on + strand, within Psyr_2992 at 3585.093 kb on + strand, within Psyr_2992 at 3585.093 kb on + strand, within Psyr_2992 at 3585.199 kb on + strand at 3585.200 kb on - strand at 3585.261 kb on + strand at 3585.261 kb on + strand at 3585.261 kb on + strand at 3585.261 kb on + strand at 3585.262 kb on - strand at 3585.262 kb on - strand at 3585.264 kb on + strand at 3585.264 kb on + strand at 3585.264 kb on + strand at 3585.265 kb on - strand at 3585.265 kb on - strand at 3585.278 kb on - strand at 3585.294 kb on + strand at 3585.304 kb on - strand at 3585.443 kb on + strand at 3585.444 kb on - strand at 3585.577 kb on + strand, within Psyr_2993 at 3585.578 kb on - strand, within Psyr_2993 at 3585.578 kb on - strand, within Psyr_2993 at 3585.631 kb on + strand, within Psyr_2993 at 3585.631 kb on + strand, within Psyr_2993 at 3585.631 kb on + strand, within Psyr_2993 at 3585.631 kb on + strand, within Psyr_2993 at 3585.631 kb on + strand, within Psyr_2993 at 3585.632 kb on - strand, within Psyr_2993 at 3585.632 kb on - strand, within Psyr_2993 at 3585.632 kb on - strand, within Psyr_2993 at 3585.632 kb on - strand, within Psyr_2993 at 3585.632 kb on - strand, within Psyr_2993 at 3585.632 kb on - strand, within Psyr_2993 at 3585.781 kb on + strand, within Psyr_2993 at 3585.782 kb on - strand, within Psyr_2993 at 3585.800 kb on - strand, within Psyr_2993 at 3585.800 kb on - strand, within Psyr_2993 at 3585.878 kb on - strand, within Psyr_2993 at 3585.878 kb on - strand, within Psyr_2993 at 3585.878 kb on - strand, within Psyr_2993 at 3585.904 kb on + strand, within Psyr_2993 at 3585.904 kb on + strand, within Psyr_2993 at 3585.904 kb on + strand, within Psyr_2993 at 3585.905 kb on - strand, within Psyr_2993 at 3585.905 kb on - strand, within Psyr_2993 at 3585.916 kb on + strand, within Psyr_2993 at 3586.264 kb on + strand, within Psyr_2993 at 3586.264 kb on + strand, within Psyr_2993 at 3586.265 kb on - strand, within Psyr_2993 at 3586.265 kb on - strand, within Psyr_2993 at 3586.330 kb on + strand, within Psyr_2993 at 3586.360 kb on - strand, within Psyr_2993 at 3586.754 kb on + strand, within Psyr_2993 at 3586.754 kb on + strand, within Psyr_2993 at 3586.754 kb on + strand, within Psyr_2993 at 3586.754 kb on + strand, within Psyr_2993 at 3586.754 kb on + strand, within Psyr_2993 at 3586.754 kb on + strand, within Psyr_2993 at 3586.754 kb on + strand, within Psyr_2993 at 3586.754 kb on + strand, within Psyr_2993 at 3586.754 kb on + strand, within Psyr_2993 at 3586.754 kb on + strand, within Psyr_2993 at 3586.755 kb on - strand, within Psyr_2993 at 3586.755 kb on - strand, within Psyr_2993 at 3586.755 kb on - strand, within Psyr_2993 at 3586.755 kb on - strand, within Psyr_2993 at 3586.755 kb on - strand, within Psyr_2993 at 3586.786 kb on + strand, within Psyr_2993 at 3586.786 kb on + strand, within Psyr_2993 at 3586.787 kb on - strand, within Psyr_2993 at 3586.787 kb on - strand, within Psyr_2993 at 3586.861 kb on - strand, within Psyr_2993 at 3586.982 kb on + strand at 3586.983 kb on - strand at 3587.039 kb on - strand at 3587.039 kb on - strand at 3587.365 kb on - strand, within Psyr_2994 at 3587.365 kb on - strand, within Psyr_2994 at 3587.564 kb on + strand, within Psyr_2994 at 3587.715 kb on - strand, within Psyr_2994 at 3587.904 kb on - strand, within Psyr_2994
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 3.125 ug/ml remove 3,584,324 - Psyr_2992 0.41 -1.2 3,584,324 - Psyr_2992 0.41 -1.9 3,584,328 + Psyr_2992 0.41 +0.1 3,584,329 - Psyr_2992 0.41 +4.3 3,584,387 + Psyr_2992 0.45 -1.3 3,584,409 + Psyr_2992 0.46 -1.0 3,584,478 + Psyr_2992 0.51 -0.6 3,584,478 + Psyr_2992 0.51 -1.1 3,584,479 - Psyr_2992 0.51 +1.8 3,584,667 + Psyr_2992 0.62 -0.7 3,584,667 + Psyr_2992 0.62 -1.0 3,584,667 + Psyr_2992 0.62 -2.4 3,584,667 + Psyr_2992 0.62 -1.6 3,584,668 - Psyr_2992 0.63 +1.5 3,584,668 - Psyr_2992 0.63 +0.2 3,584,668 - Psyr_2992 0.63 -2.0 3,584,982 + Psyr_2992 0.82 +0.5 3,584,982 + Psyr_2992 0.82 +3.2 3,584,982 + Psyr_2992 0.82 +1.2 3,584,983 - Psyr_2992 0.82 -3.0 3,584,983 - Psyr_2992 0.82 +0.8 3,585,020 + Psyr_2992 0.85 -1.3 3,585,093 + Psyr_2992 0.89 -4.2 3,585,093 + Psyr_2992 0.89 +0.4 3,585,199 + -2.5 3,585,200 - +0.7 3,585,261 + +0.2 3,585,261 + +3.9 3,585,261 + +3.8 3,585,261 + +0.6 3,585,262 - +3.0 3,585,262 - -0.9 3,585,264 + -2.1 3,585,264 + -1.6 3,585,264 + +1.1 3,585,265 - +0.7 3,585,265 - +0.2 3,585,278 - -0.8 3,585,294 + -1.1 3,585,304 - -0.3 3,585,443 + -0.4 3,585,444 - +0.7 3,585,577 + Psyr_2993 0.16 +0.8 3,585,578 - Psyr_2993 0.16 +1.2 3,585,578 - Psyr_2993 0.16 +0.1 3,585,631 + Psyr_2993 0.19 -0.2 3,585,631 + Psyr_2993 0.19 +1.0 3,585,631 + Psyr_2993 0.19 +0.2 3,585,631 + Psyr_2993 0.19 -1.3 3,585,631 + Psyr_2993 0.19 -0.7 3,585,632 - Psyr_2993 0.19 -0.2 3,585,632 - Psyr_2993 0.19 +1.3 3,585,632 - Psyr_2993 0.19 +2.9 3,585,632 - Psyr_2993 0.19 +0.2 3,585,632 - Psyr_2993 0.19 +1.2 3,585,632 - Psyr_2993 0.19 -0.5 3,585,781 + Psyr_2993 0.27 +0.2 3,585,782 - Psyr_2993 0.27 +1.3 3,585,800 - Psyr_2993 0.28 +1.5 3,585,800 - Psyr_2993 0.28 +0.4 3,585,878 - Psyr_2993 0.33 -0.8 3,585,878 - Psyr_2993 0.33 +0.2 3,585,878 - Psyr_2993 0.33 +2.5 3,585,904 + Psyr_2993 0.34 -4.5 3,585,904 + Psyr_2993 0.34 -1.5 3,585,904 + Psyr_2993 0.34 +0.2 3,585,905 - Psyr_2993 0.34 -3.6 3,585,905 - Psyr_2993 0.34 +1.1 3,585,916 + Psyr_2993 0.35 -0.9 3,586,264 + Psyr_2993 0.54 +0.2 3,586,264 + Psyr_2993 0.54 -3.7 3,586,265 - Psyr_2993 0.54 -1.1 3,586,265 - Psyr_2993 0.54 +0.2 3,586,330 + Psyr_2993 0.58 +0.7 3,586,360 - Psyr_2993 0.59 +0.2 3,586,754 + Psyr_2993 0.81 +3.5 3,586,754 + Psyr_2993 0.81 +2.5 3,586,754 + Psyr_2993 0.81 -2.3 3,586,754 + Psyr_2993 0.81 +3.9 3,586,754 + Psyr_2993 0.81 -0.9 3,586,754 + Psyr_2993 0.81 -0.5 3,586,754 + Psyr_2993 0.81 +0.9 3,586,754 + Psyr_2993 0.81 -1.0 3,586,754 + Psyr_2993 0.81 -0.4 3,586,754 + Psyr_2993 0.81 -2.1 3,586,755 - Psyr_2993 0.81 +0.2 3,586,755 - Psyr_2993 0.81 +0.1 3,586,755 - Psyr_2993 0.81 +3.7 3,586,755 - Psyr_2993 0.81 -0.5 3,586,755 - Psyr_2993 0.81 -0.6 3,586,786 + Psyr_2993 0.83 -0.5 3,586,786 + Psyr_2993 0.83 +2.8 3,586,787 - Psyr_2993 0.83 -0.6 3,586,787 - Psyr_2993 0.83 +1.7 3,586,861 - Psyr_2993 0.87 -0.2 3,586,982 + -0.6 3,586,983 - +0.8 3,587,039 - +3.0 3,587,039 - -1.8 3,587,365 - Psyr_2994 0.13 +3.2 3,587,365 - Psyr_2994 0.13 -2.0 3,587,564 + Psyr_2994 0.23 -2.8 3,587,715 - Psyr_2994 0.30 +0.7 3,587,904 - Psyr_2994 0.39 -2.0
Or see this region's nucleotide sequence