Experiment: KB with Acridine orange 3.125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2529 and Psyr_2530 overlap by 4 nucleotides Psyr_2530 and Psyr_2531 are separated by 188 nucleotides
Psyr_2529: Psyr_2529 - Amidohydrolase, at 2,940,346 to 2,941,605
_2529
Psyr_2530: Psyr_2530 - General substrate transporter:Major facilitator superfamily, at 2,941,602 to 2,942,912
_2530
Psyr_2531: Psyr_2531 - transcriptional regulator, LysR family, at 2,943,101 to 2,944,012
_2531
Position (kb)
2941
2942
2943 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2940.908 kb on + strand, within Psyr_2529 at 2940.908 kb on + strand, within Psyr_2529 at 2940.908 kb on + strand, within Psyr_2529 at 2940.909 kb on - strand, within Psyr_2529 at 2940.909 kb on - strand, within Psyr_2529 at 2940.909 kb on - strand, within Psyr_2529 at 2941.332 kb on - strand, within Psyr_2529 at 2941.486 kb on + strand at 2941.486 kb on + strand at 2941.487 kb on - strand at 2941.487 kb on - strand at 2941.699 kb on + strand at 2941.699 kb on + strand at 2941.699 kb on + strand at 2941.700 kb on - strand at 2941.792 kb on + strand, within Psyr_2530 at 2941.792 kb on + strand, within Psyr_2530 at 2941.867 kb on + strand, within Psyr_2530 at 2941.867 kb on + strand, within Psyr_2530 at 2941.867 kb on + strand, within Psyr_2530 at 2941.868 kb on - strand, within Psyr_2530 at 2942.131 kb on + strand, within Psyr_2530 at 2942.132 kb on - strand, within Psyr_2530 at 2942.146 kb on + strand, within Psyr_2530 at 2942.146 kb on + strand, within Psyr_2530 at 2942.146 kb on + strand, within Psyr_2530 at 2942.146 kb on + strand, within Psyr_2530 at 2942.254 kb on + strand, within Psyr_2530 at 2942.254 kb on + strand, within Psyr_2530 at 2942.255 kb on - strand, within Psyr_2530 at 2942.255 kb on - strand, within Psyr_2530 at 2942.255 kb on - strand, within Psyr_2530 at 2942.286 kb on - strand, within Psyr_2530 at 2942.287 kb on + strand, within Psyr_2530 at 2942.288 kb on - strand, within Psyr_2530 at 2942.368 kb on + strand, within Psyr_2530 at 2942.444 kb on - strand, within Psyr_2530 at 2942.564 kb on - strand, within Psyr_2530 at 2942.589 kb on - strand, within Psyr_2530 at 2942.668 kb on + strand, within Psyr_2530 at 2942.668 kb on + strand, within Psyr_2530 at 2942.668 kb on + strand, within Psyr_2530 at 2942.668 kb on + strand, within Psyr_2530 at 2942.668 kb on + strand, within Psyr_2530 at 2942.668 kb on + strand, within Psyr_2530 at 2942.668 kb on + strand, within Psyr_2530 at 2942.668 kb on + strand, within Psyr_2530 at 2942.668 kb on + strand, within Psyr_2530 at 2942.668 kb on + strand, within Psyr_2530 at 2942.669 kb on - strand, within Psyr_2530 at 2942.669 kb on - strand, within Psyr_2530 at 2942.669 kb on - strand, within Psyr_2530 at 2942.669 kb on - strand, within Psyr_2530 at 2942.669 kb on - strand, within Psyr_2530 at 2942.669 kb on - strand, within Psyr_2530 at 2942.741 kb on - strand, within Psyr_2530 at 2942.901 kb on + strand at 2942.901 kb on + strand at 2942.901 kb on + strand at 2942.902 kb on - strand at 2942.902 kb on - strand at 2942.935 kb on - strand at 2943.063 kb on + strand at 2943.064 kb on - strand at 2943.198 kb on + strand, within Psyr_2531 at 2943.201 kb on + strand, within Psyr_2531 at 2943.257 kb on + strand, within Psyr_2531 at 2943.257 kb on + strand, within Psyr_2531 at 2943.257 kb on + strand, within Psyr_2531 at 2943.257 kb on + strand, within Psyr_2531 at 2943.258 kb on - strand, within Psyr_2531 at 2943.258 kb on - strand, within Psyr_2531
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 3.125 ug/ml remove 2,940,908 + Psyr_2529 0.45 -2.8 2,940,908 + Psyr_2529 0.45 -3.2 2,940,908 + Psyr_2529 0.45 -2.1 2,940,909 - Psyr_2529 0.45 +0.1 2,940,909 - Psyr_2529 0.45 +1.3 2,940,909 - Psyr_2529 0.45 +0.2 2,941,332 - Psyr_2529 0.78 +3.5 2,941,486 + +3.2 2,941,486 + -0.8 2,941,487 - -0.1 2,941,487 - -0.3 2,941,699 + +1.9 2,941,699 + +1.4 2,941,699 + +2.0 2,941,700 - -1.4 2,941,792 + Psyr_2530 0.14 -2.9 2,941,792 + Psyr_2530 0.14 +1.8 2,941,867 + Psyr_2530 0.20 -0.4 2,941,867 + Psyr_2530 0.20 -4.4 2,941,867 + Psyr_2530 0.20 -1.2 2,941,868 - Psyr_2530 0.20 -0.4 2,942,131 + Psyr_2530 0.40 -0.4 2,942,132 - Psyr_2530 0.40 +3.2 2,942,146 + Psyr_2530 0.41 +0.4 2,942,146 + Psyr_2530 0.41 +4.5 2,942,146 + Psyr_2530 0.41 +0.2 2,942,146 + Psyr_2530 0.41 +1.2 2,942,254 + Psyr_2530 0.50 +3.7 2,942,254 + Psyr_2530 0.50 +3.0 2,942,255 - Psyr_2530 0.50 -0.4 2,942,255 - Psyr_2530 0.50 -2.1 2,942,255 - Psyr_2530 0.50 +2.1 2,942,286 - Psyr_2530 0.52 -1.0 2,942,287 + Psyr_2530 0.52 +4.2 2,942,288 - Psyr_2530 0.52 -2.8 2,942,368 + Psyr_2530 0.58 +2.0 2,942,444 - Psyr_2530 0.64 +0.2 2,942,564 - Psyr_2530 0.73 +0.0 2,942,589 - Psyr_2530 0.75 -3.1 2,942,668 + Psyr_2530 0.81 -3.5 2,942,668 + Psyr_2530 0.81 +0.2 2,942,668 + Psyr_2530 0.81 +0.8 2,942,668 + Psyr_2530 0.81 +3.8 2,942,668 + Psyr_2530 0.81 -1.2 2,942,668 + Psyr_2530 0.81 +0.4 2,942,668 + Psyr_2530 0.81 +1.8 2,942,668 + Psyr_2530 0.81 -4.7 2,942,668 + Psyr_2530 0.81 -2.9 2,942,668 + Psyr_2530 0.81 -1.1 2,942,669 - Psyr_2530 0.81 +1.8 2,942,669 - Psyr_2530 0.81 -2.8 2,942,669 - Psyr_2530 0.81 +0.6 2,942,669 - Psyr_2530 0.81 -0.2 2,942,669 - Psyr_2530 0.81 +2.8 2,942,669 - Psyr_2530 0.81 +0.4 2,942,741 - Psyr_2530 0.87 +0.2 2,942,901 + -1.4 2,942,901 + +2.8 2,942,901 + -2.6 2,942,902 - +0.4 2,942,902 - +0.2 2,942,935 - -4.1 2,943,063 + -0.3 2,943,064 - -1.2 2,943,198 + Psyr_2531 0.11 -2.6 2,943,201 + Psyr_2531 0.11 +0.6 2,943,257 + Psyr_2531 0.17 +1.8 2,943,257 + Psyr_2531 0.17 -0.4 2,943,257 + Psyr_2531 0.17 +3.0 2,943,257 + Psyr_2531 0.17 -1.9 2,943,258 - Psyr_2531 0.17 -1.2 2,943,258 - Psyr_2531 0.17 -1.4
Or see this region's nucleotide sequence