Experiment: KB with Acridine orange 3.125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2237 and Psyr_2238 are separated by 18 nucleotides Psyr_2238 and Psyr_2239 are separated by 97 nucleotides
Psyr_2237: Psyr_2237 - Histidine kinase, HAMP region:Bacterial chemotaxis sensory transducer, at 2,599,432 to 2,601,417
_2237
Psyr_2238: Psyr_2238 - monosaccharide ABC transporter substrate-binding protein, CUT2 family, at 2,601,436 to 2,602,356
_2238
Psyr_2239: Psyr_2239 - DNA/RNA non-specific endonuclease, at 2,602,454 to 2,603,398
_2239
Position (kb)
2601
2602
2603 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 2601.518 kb on + strand at 2601.519 kb on - strand at 2601.519 kb on - strand at 2601.532 kb on + strand, within Psyr_2238 at 2601.533 kb on - strand, within Psyr_2238 at 2601.533 kb on - strand, within Psyr_2238 at 2601.533 kb on - strand, within Psyr_2238 at 2601.535 kb on - strand, within Psyr_2238 at 2601.581 kb on + strand, within Psyr_2238 at 2601.874 kb on + strand, within Psyr_2238 at 2601.874 kb on + strand, within Psyr_2238 at 2601.874 kb on + strand, within Psyr_2238 at 2601.874 kb on + strand, within Psyr_2238 at 2601.875 kb on - strand, within Psyr_2238 at 2601.875 kb on - strand, within Psyr_2238 at 2601.875 kb on - strand, within Psyr_2238 at 2601.964 kb on + strand, within Psyr_2238 at 2602.326 kb on + strand at 2602.662 kb on + strand, within Psyr_2239 at 2602.663 kb on - strand, within Psyr_2239 at 2602.663 kb on - strand, within Psyr_2239 at 2602.663 kb on - strand, within Psyr_2239 at 2602.663 kb on - strand, within Psyr_2239 at 2602.704 kb on - strand, within Psyr_2239 at 2602.824 kb on + strand, within Psyr_2239 at 2602.825 kb on - strand, within Psyr_2239 at 2602.825 kb on - strand, within Psyr_2239 at 2602.825 kb on - strand, within Psyr_2239 at 2602.825 kb on - strand, within Psyr_2239 at 2602.983 kb on + strand, within Psyr_2239 at 2603.060 kb on + strand, within Psyr_2239 at 2603.060 kb on + strand, within Psyr_2239 at 2603.060 kb on + strand, within Psyr_2239 at 2603.060 kb on + strand, within Psyr_2239 at 2603.061 kb on - strand, within Psyr_2239 at 2603.061 kb on - strand, within Psyr_2239 at 2603.094 kb on + strand, within Psyr_2239 at 2603.095 kb on - strand, within Psyr_2239 at 2603.095 kb on - strand, within Psyr_2239 at 2603.175 kb on + strand, within Psyr_2239 at 2603.176 kb on - strand, within Psyr_2239
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 3.125 ug/ml remove 2,601,518 + +0.2 2,601,519 - +4.1 2,601,519 - +2.0 2,601,532 + Psyr_2238 0.10 +3.2 2,601,533 - Psyr_2238 0.11 -2.6 2,601,533 - Psyr_2238 0.11 -0.4 2,601,533 - Psyr_2238 0.11 +0.2 2,601,535 - Psyr_2238 0.11 -1.2 2,601,581 + Psyr_2238 0.16 -0.5 2,601,874 + Psyr_2238 0.48 +3.8 2,601,874 + Psyr_2238 0.48 -3.0 2,601,874 + Psyr_2238 0.48 +1.2 2,601,874 + Psyr_2238 0.48 -0.8 2,601,875 - Psyr_2238 0.48 -1.1 2,601,875 - Psyr_2238 0.48 +0.7 2,601,875 - Psyr_2238 0.48 +0.2 2,601,964 + Psyr_2238 0.57 +2.8 2,602,326 + -3.0 2,602,662 + Psyr_2239 0.22 -0.9 2,602,663 - Psyr_2239 0.22 -2.8 2,602,663 - Psyr_2239 0.22 +1.5 2,602,663 - Psyr_2239 0.22 +1.4 2,602,663 - Psyr_2239 0.22 +0.2 2,602,704 - Psyr_2239 0.26 +4.9 2,602,824 + Psyr_2239 0.39 -1.0 2,602,825 - Psyr_2239 0.39 -4.1 2,602,825 - Psyr_2239 0.39 -3.4 2,602,825 - Psyr_2239 0.39 +0.2 2,602,825 - Psyr_2239 0.39 -1.1 2,602,983 + Psyr_2239 0.56 +0.2 2,603,060 + Psyr_2239 0.64 -0.6 2,603,060 + Psyr_2239 0.64 +0.5 2,603,060 + Psyr_2239 0.64 -0.7 2,603,060 + Psyr_2239 0.64 +0.2 2,603,061 - Psyr_2239 0.64 -0.8 2,603,061 - Psyr_2239 0.64 -2.1 2,603,094 + Psyr_2239 0.68 -0.8 2,603,095 - Psyr_2239 0.68 -0.9 2,603,095 - Psyr_2239 0.68 -2.4 2,603,175 + Psyr_2239 0.76 +3.7 2,603,176 - Psyr_2239 0.76 +0.8
Or see this region's nucleotide sequence