Strain Fitness in Pseudomonas syringae pv. syringae B728a ΔmexB around Psyr_1735

Experiment: KB with Acridine orange 3.125 ug/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_1734 and Psyr_1735 are separated by 24 nucleotidesPsyr_1735 and Psyr_1736 are separated by 106 nucleotides Psyr_1734: Psyr_1734 - glutaminyl-tRNA synthetase, at 1,968,763 to 1,970,457 _1734 Psyr_1735: Psyr_1735 - cysteinyl-tRNA synthetase, at 1,970,482 to 1,971,864 _1735 Psyr_1736: Psyr_1736 - Helix-turn-helix, Fis-type, at 1,971,971 to 1,973,824 _1736 Position (kb) 1970 1971 1972Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5at 1970.463 kb on + strandat 1970.463 kb on + strandat 1971.884 kb on + strandat 1971.884 kb on + strandat 1971.884 kb on + strandat 1971.884 kb on + strandat 1971.885 kb on - strandat 1972.002 kb on + strandat 1972.002 kb on + strandat 1972.003 kb on - strandat 1972.003 kb on - strandat 1972.280 kb on + strand, within Psyr_1736at 1972.419 kb on + strand, within Psyr_1736at 1972.419 kb on + strand, within Psyr_1736at 1972.490 kb on + strand, within Psyr_1736at 1972.491 kb on - strand, within Psyr_1736at 1972.491 kb on - strand, within Psyr_1736at 1972.491 kb on - strand, within Psyr_1736at 1972.491 kb on - strand, within Psyr_1736at 1972.491 kb on - strand, within Psyr_1736at 1972.491 kb on - strand, within Psyr_1736

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Acridine orange 3.125 ug/ml
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1,970,463 + -1.3
1,970,463 + +0.3
1,971,884 + +1.5
1,971,884 + +0.6
1,971,884 + +5.5
1,971,884 + -1.6
1,971,885 - +1.5
1,972,002 + +1.9
1,972,002 + -0.5
1,972,003 - +0.5
1,972,003 - -1.1
1,972,280 + Psyr_1736 0.17 -0.8
1,972,419 + Psyr_1736 0.24 -1.6
1,972,419 + Psyr_1736 0.24 -1.2
1,972,490 + Psyr_1736 0.28 +3.1
1,972,491 - Psyr_1736 0.28 +1.4
1,972,491 - Psyr_1736 0.28 -1.2
1,972,491 - Psyr_1736 0.28 -1.9
1,972,491 - Psyr_1736 0.28 +3.5
1,972,491 - Psyr_1736 0.28 +1.1
1,972,491 - Psyr_1736 0.28 +3.1

Or see this region's nucleotide sequence