Experiment: KB with Acridine orange 3.125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1613 and Psyr_1614 are separated by 120 nucleotides Psyr_1614 and Psyr_1615 are separated by 32 nucleotides
Psyr_1613: Psyr_1613 - septum site-determining protein MinC, at 1,815,888 to 1,816,628
_1613
Psyr_1614: Psyr_1614 - lipid A biosynthesis acyltransferase, at 1,816,749 to 1,817,681
_1614
Psyr_1615: Psyr_1615 - Patatin, at 1,817,714 to 1,818,898
_1615
Position (kb)
1816
1817
1818 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 1815.764 kb on - strand at 1815.775 kb on - strand at 1815.790 kb on + strand at 1815.791 kb on - strand at 1815.791 kb on - strand at 1815.791 kb on - strand at 1815.791 kb on - strand at 1815.889 kb on + strand at 1815.982 kb on + strand, within Psyr_1613 at 1815.982 kb on + strand, within Psyr_1613 at 1815.983 kb on - strand, within Psyr_1613 at 1815.983 kb on - strand, within Psyr_1613 at 1816.168 kb on + strand, within Psyr_1613 at 1816.169 kb on - strand, within Psyr_1613 at 1816.169 kb on - strand, within Psyr_1613 at 1816.473 kb on - strand, within Psyr_1613 at 1816.636 kb on - strand at 1816.637 kb on + strand at 1816.637 kb on + strand at 1816.637 kb on + strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.671 kb on + strand at 1816.671 kb on + strand at 1816.671 kb on + strand at 1816.671 kb on + strand at 1816.672 kb on - strand at 1816.672 kb on - strand at 1816.674 kb on - strand at 1816.674 kb on - strand at 1816.674 kb on - strand at 1816.766 kb on + strand at 1816.766 kb on + strand at 1816.890 kb on + strand, within Psyr_1614 at 1816.890 kb on + strand, within Psyr_1614 at 1816.890 kb on + strand, within Psyr_1614 at 1816.891 kb on - strand, within Psyr_1614 at 1817.202 kb on + strand, within Psyr_1614 at 1817.202 kb on + strand, within Psyr_1614 at 1817.203 kb on - strand, within Psyr_1614 at 1817.315 kb on - strand, within Psyr_1614 at 1817.315 kb on - strand, within Psyr_1614 at 1817.358 kb on + strand, within Psyr_1614 at 1817.525 kb on + strand, within Psyr_1614 at 1817.664 kb on + strand at 1817.664 kb on + strand at 1817.664 kb on + strand at 1817.665 kb on - strand at 1817.665 kb on - strand at 1817.665 kb on - strand at 1817.665 kb on - strand at 1817.675 kb on + strand at 1817.675 kb on + strand at 1817.675 kb on + strand at 1817.675 kb on + strand at 1817.676 kb on - strand at 1817.676 kb on - strand at 1817.679 kb on + strand at 1817.680 kb on - strand at 1817.680 kb on - strand at 1817.680 kb on - strand at 1817.680 kb on - strand at 1817.711 kb on + strand at 1817.711 kb on + strand at 1817.711 kb on + strand at 1817.711 kb on + strand at 1817.712 kb on - strand at 1817.712 kb on - strand at 1817.712 kb on - strand at 1817.712 kb on - strand at 1817.712 kb on - strand at 1817.712 kb on - strand at 1817.808 kb on - strand at 1817.997 kb on + strand, within Psyr_1615 at 1818.259 kb on - strand, within Psyr_1615 at 1818.259 kb on - strand, within Psyr_1615 at 1818.362 kb on + strand, within Psyr_1615 at 1818.362 kb on + strand, within Psyr_1615 at 1818.363 kb on - strand, within Psyr_1615 at 1818.363 kb on - strand, within Psyr_1615 at 1818.363 kb on - strand, within Psyr_1615 at 1818.417 kb on + strand, within Psyr_1615 at 1818.417 kb on + strand, within Psyr_1615 at 1818.470 kb on - strand, within Psyr_1615 at 1818.562 kb on + strand, within Psyr_1615
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 3.125 ug/ml remove 1,815,764 - -4.2 1,815,775 - -0.5 1,815,790 + +0.4 1,815,791 - +1.4 1,815,791 - +0.5 1,815,791 - +2.4 1,815,791 - -0.2 1,815,889 + +0.4 1,815,982 + Psyr_1613 0.13 +0.4 1,815,982 + Psyr_1613 0.13 -0.6 1,815,983 - Psyr_1613 0.13 +0.4 1,815,983 - Psyr_1613 0.13 +0.4 1,816,168 + Psyr_1613 0.38 -3.0 1,816,169 - Psyr_1613 0.38 -1.2 1,816,169 - Psyr_1613 0.38 -3.6 1,816,473 - Psyr_1613 0.79 -3.6 1,816,636 - +3.4 1,816,637 + -1.6 1,816,637 + -2.8 1,816,637 + +0.4 1,816,638 - +0.7 1,816,638 - +2.7 1,816,638 - +1.4 1,816,638 - +4.7 1,816,638 - -4.5 1,816,638 - -1.0 1,816,638 - -0.4 1,816,638 - +0.0 1,816,638 - -2.1 1,816,638 - -0.6 1,816,638 - -3.5 1,816,638 - -2.6 1,816,638 - +0.4 1,816,638 - -0.4 1,816,671 + +0.4 1,816,671 + +0.4 1,816,671 + -3.1 1,816,671 + -2.0 1,816,672 - -1.0 1,816,672 - -0.7 1,816,674 - +0.4 1,816,674 - -2.3 1,816,674 - -0.9 1,816,766 + -2.2 1,816,766 + +0.4 1,816,890 + Psyr_1614 0.15 -3.9 1,816,890 + Psyr_1614 0.15 -4.9 1,816,890 + Psyr_1614 0.15 -4.4 1,816,891 - Psyr_1614 0.15 -2.6 1,817,202 + Psyr_1614 0.49 -3.9 1,817,202 + Psyr_1614 0.49 -2.7 1,817,203 - Psyr_1614 0.49 -3.9 1,817,315 - Psyr_1614 0.61 +0.0 1,817,315 - Psyr_1614 0.61 +0.4 1,817,358 + Psyr_1614 0.65 -2.2 1,817,525 + Psyr_1614 0.83 -1.1 1,817,664 + -1.6 1,817,664 + +2.4 1,817,664 + +0.1 1,817,665 - -3.7 1,817,665 - -3.1 1,817,665 - +2.4 1,817,665 - -0.4 1,817,675 + +0.5 1,817,675 + -0.6 1,817,675 + -1.4 1,817,675 + +2.4 1,817,676 - +2.9 1,817,676 - -1.5 1,817,679 + +0.9 1,817,680 - -4.5 1,817,680 - +4.3 1,817,680 - +2.4 1,817,680 - +0.4 1,817,711 + -1.0 1,817,711 + -0.1 1,817,711 + -0.9 1,817,711 + -0.9 1,817,712 - -0.5 1,817,712 - -0.2 1,817,712 - +1.4 1,817,712 - -0.3 1,817,712 - -1.0 1,817,712 - -0.4 1,817,808 - -0.8 1,817,997 + Psyr_1615 0.24 +1.4 1,818,259 - Psyr_1615 0.46 +3.8 1,818,259 - Psyr_1615 0.46 -0.7 1,818,362 + Psyr_1615 0.55 +0.8 1,818,362 + Psyr_1615 0.55 -0.5 1,818,363 - Psyr_1615 0.55 -0.5 1,818,363 - Psyr_1615 0.55 +2.4 1,818,363 - Psyr_1615 0.55 +0.1 1,818,417 + Psyr_1615 0.59 -1.2 1,818,417 + Psyr_1615 0.59 +2.9 1,818,470 - Psyr_1615 0.64 -0.8 1,818,562 + Psyr_1615 0.72 -2.0
Or see this region's nucleotide sequence