Experiment: KB with Acridine orange 3.125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1375 and Psyr_1376 are separated by 259 nucleotides Psyr_1376 and Psyr_1377 are separated by 396 nucleotides
Psyr_1375: Psyr_1375 - 4Fe-4S ferredoxin, iron-sulfur binding protein, at 1,558,249 to 1,558,572
_1375
Psyr_1376: Psyr_1376 - DNA mismatch repair protein MutS, at 1,558,832 to 1,561,411
_1376
Psyr_1377: Psyr_1377 - CinA, C-terminal, at 1,561,808 to 1,562,386
_1377
Position (kb) 1558
1559
1560
1561
1562 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 1557.832 kb on + strand at 1557.836 kb on + strand at 1557.836 kb on + strand at 1557.836 kb on + strand at 1557.836 kb on + strand at 1557.837 kb on - strand at 1557.837 kb on - strand at 1557.837 kb on - strand at 1557.837 kb on - strand at 1557.846 kb on + strand at 1557.852 kb on + strand at 1557.852 kb on + strand at 1557.852 kb on + strand at 1557.852 kb on + strand at 1557.853 kb on - strand at 1557.868 kb on + strand at 1557.869 kb on - strand at 1557.892 kb on - strand at 1557.892 kb on - strand at 1557.892 kb on - strand at 1557.974 kb on - strand at 1558.133 kb on - strand at 1558.139 kb on + strand at 1558.140 kb on - strand at 1558.707 kb on - strand at 1558.811 kb on - strand at 1558.817 kb on - strand at 1558.817 kb on - strand at 1558.817 kb on - strand at 1558.817 kb on - strand at 1558.817 kb on - strand at 1558.817 kb on - strand at 1558.854 kb on + strand at 1558.854 kb on + strand at 1558.854 kb on + strand at 1558.854 kb on + strand at 1558.855 kb on - strand at 1558.855 kb on - strand at 1558.855 kb on - strand at 1558.886 kb on - strand at 1558.945 kb on + strand at 1558.945 kb on + strand at 1558.946 kb on - strand at 1558.946 kb on - strand at 1558.946 kb on - strand at 1559.095 kb on - strand, within Psyr_1376 at 1559.149 kb on + strand, within Psyr_1376 at 1559.241 kb on + strand, within Psyr_1376 at 1559.510 kb on + strand, within Psyr_1376 at 1559.510 kb on + strand, within Psyr_1376 at 1559.533 kb on + strand, within Psyr_1376 at 1559.534 kb on - strand, within Psyr_1376 at 1559.534 kb on - strand, within Psyr_1376 at 1559.534 kb on - strand, within Psyr_1376 at 1559.535 kb on + strand, within Psyr_1376 at 1559.535 kb on + strand, within Psyr_1376 at 1559.535 kb on + strand, within Psyr_1376 at 1559.535 kb on + strand, within Psyr_1376 at 1559.535 kb on + strand, within Psyr_1376 at 1559.535 kb on + strand, within Psyr_1376 at 1559.535 kb on + strand, within Psyr_1376 at 1559.535 kb on + strand, within Psyr_1376 at 1559.535 kb on + strand, within Psyr_1376 at 1559.535 kb on + strand, within Psyr_1376 at 1559.535 kb on + strand, within Psyr_1376 at 1559.535 kb on + strand, within Psyr_1376 at 1559.535 kb on + strand, within Psyr_1376 at 1559.535 kb on + strand, within Psyr_1376 at 1559.535 kb on + strand, within Psyr_1376 at 1559.535 kb on + strand, within Psyr_1376 at 1559.535 kb on + strand, within Psyr_1376 at 1559.535 kb on + strand, within Psyr_1376 at 1559.535 kb on + strand, within Psyr_1376 at 1559.535 kb on + strand, within Psyr_1376 at 1559.536 kb on - strand, within Psyr_1376 at 1559.536 kb on - strand, within Psyr_1376 at 1559.536 kb on - strand, within Psyr_1376 at 1559.536 kb on - strand, within Psyr_1376 at 1559.536 kb on - strand, within Psyr_1376 at 1559.536 kb on - strand, within Psyr_1376 at 1559.536 kb on - strand, within Psyr_1376 at 1559.536 kb on - strand, within Psyr_1376 at 1559.536 kb on - strand, within Psyr_1376 at 1559.536 kb on - strand, within Psyr_1376 at 1559.628 kb on - strand, within Psyr_1376 at 1559.770 kb on + strand, within Psyr_1376 at 1559.771 kb on - strand, within Psyr_1376 at 1559.821 kb on - strand, within Psyr_1376 at 1559.934 kb on - strand, within Psyr_1376 at 1559.934 kb on - strand, within Psyr_1376 at 1559.934 kb on - strand, within Psyr_1376 at 1559.934 kb on - strand, within Psyr_1376 at 1559.934 kb on - strand, within Psyr_1376 at 1559.934 kb on - strand, within Psyr_1376 at 1559.937 kb on + strand, within Psyr_1376 at 1559.937 kb on + strand, within Psyr_1376 at 1559.937 kb on + strand, within Psyr_1376 at 1559.937 kb on + strand, within Psyr_1376 at 1559.937 kb on + strand, within Psyr_1376 at 1559.937 kb on + strand, within Psyr_1376 at 1559.938 kb on - strand, within Psyr_1376 at 1559.938 kb on - strand, within Psyr_1376 at 1559.938 kb on - strand, within Psyr_1376 at 1559.938 kb on - strand, within Psyr_1376 at 1559.986 kb on - strand, within Psyr_1376 at 1559.986 kb on - strand, within Psyr_1376 at 1560.204 kb on + strand, within Psyr_1376 at 1560.205 kb on - strand, within Psyr_1376 at 1560.205 kb on - strand, within Psyr_1376 at 1560.644 kb on + strand, within Psyr_1376 at 1560.645 kb on - strand, within Psyr_1376 at 1560.681 kb on + strand, within Psyr_1376 at 1560.681 kb on + strand, within Psyr_1376 at 1560.681 kb on + strand, within Psyr_1376 at 1560.968 kb on + strand, within Psyr_1376 at 1561.173 kb on + strand at 1561.191 kb on + strand at 1561.250 kb on + strand at 1561.250 kb on + strand at 1561.250 kb on + strand at 1561.251 kb on - strand at 1561.288 kb on - strand at 1561.300 kb on - strand at 1561.500 kb on + strand at 1561.501 kb on - strand at 1561.547 kb on + strand at 1561.548 kb on - strand at 1561.565 kb on + strand at 1561.614 kb on + strand at 1561.614 kb on + strand at 1561.614 kb on + strand at 1561.614 kb on + strand at 1561.654 kb on + strand at 1561.654 kb on + strand at 1561.655 kb on - strand at 1561.655 kb on - strand at 1561.670 kb on + strand at 1561.670 kb on + strand at 1561.671 kb on - strand at 1561.672 kb on + strand at 1561.672 kb on + strand at 1561.799 kb on + strand at 1561.799 kb on + strand at 1561.799 kb on + strand at 1561.799 kb on + strand at 1561.799 kb on + strand at 1561.800 kb on - strand at 1561.800 kb on - strand at 1561.800 kb on - strand at 1561.969 kb on + strand, within Psyr_1377 at 1562.045 kb on + strand, within Psyr_1377 at 1562.046 kb on - strand, within Psyr_1377 at 1562.088 kb on + strand, within Psyr_1377 at 1562.088 kb on + strand, within Psyr_1377 at 1562.088 kb on + strand, within Psyr_1377 at 1562.089 kb on - strand, within Psyr_1377 at 1562.089 kb on - strand, within Psyr_1377 at 1562.089 kb on - strand, within Psyr_1377 at 1562.350 kb on - strand at 1562.410 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 3.125 ug/ml remove 1,557,832 + +5.0 1,557,836 + -0.5 1,557,836 + +2.0 1,557,836 + +1.6 1,557,836 + -1.6 1,557,837 - +0.3 1,557,837 - +0.4 1,557,837 - -0.6 1,557,837 - +1.9 1,557,846 + +0.2 1,557,852 + -1.1 1,557,852 + -0.1 1,557,852 + +3.7 1,557,852 + +2.1 1,557,853 - +0.7 1,557,868 + +1.3 1,557,869 - +2.7 1,557,892 - +1.3 1,557,892 - +0.9 1,557,892 - -1.2 1,557,974 - +0.6 1,558,133 - +3.4 1,558,139 + -1.6 1,558,140 - -2.8 1,558,707 - -1.2 1,558,811 - +4.0 1,558,817 - +2.7 1,558,817 - -1.8 1,558,817 - -4.0 1,558,817 - -0.2 1,558,817 - -3.5 1,558,817 - -0.5 1,558,854 + +3.4 1,558,854 + -0.7 1,558,854 + -1.6 1,558,854 + +4.3 1,558,855 - -0.5 1,558,855 - -0.4 1,558,855 - +2.4 1,558,886 - +1.7 1,558,945 + -0.5 1,558,945 + +5.1 1,558,946 - -1.3 1,558,946 - +0.7 1,558,946 - -3.0 1,559,095 - Psyr_1376 0.10 -0.0 1,559,149 + Psyr_1376 0.12 +2.1 1,559,241 + Psyr_1376 0.16 +5.5 1,559,510 + Psyr_1376 0.26 -0.8 1,559,510 + Psyr_1376 0.26 +2.4 1,559,533 + Psyr_1376 0.27 -1.2 1,559,534 - Psyr_1376 0.27 +0.4 1,559,534 - Psyr_1376 0.27 +2.2 1,559,534 - Psyr_1376 0.27 -3.1 1,559,535 + Psyr_1376 0.27 +0.4 1,559,535 + Psyr_1376 0.27 +0.3 1,559,535 + Psyr_1376 0.27 -4.1 1,559,535 + Psyr_1376 0.27 +3.4 1,559,535 + Psyr_1376 0.27 +2.0 1,559,535 + Psyr_1376 0.27 +0.8 1,559,535 + Psyr_1376 0.27 +0.6 1,559,535 + Psyr_1376 0.27 +1.9 1,559,535 + Psyr_1376 0.27 +0.6 1,559,535 + Psyr_1376 0.27 +0.1 1,559,535 + Psyr_1376 0.27 +0.1 1,559,535 + Psyr_1376 0.27 +0.6 1,559,535 + Psyr_1376 0.27 -0.1 1,559,535 + Psyr_1376 0.27 -3.5 1,559,535 + Psyr_1376 0.27 -1.0 1,559,535 + Psyr_1376 0.27 +0.4 1,559,535 + Psyr_1376 0.27 -0.2 1,559,535 + Psyr_1376 0.27 +3.9 1,559,535 + Psyr_1376 0.27 -0.4 1,559,535 + Psyr_1376 0.27 +0.1 1,559,536 - Psyr_1376 0.27 +1.1 1,559,536 - Psyr_1376 0.27 -0.7 1,559,536 - Psyr_1376 0.27 +0.7 1,559,536 - Psyr_1376 0.27 +1.2 1,559,536 - Psyr_1376 0.27 -0.7 1,559,536 - Psyr_1376 0.27 -0.2 1,559,536 - Psyr_1376 0.27 +4.0 1,559,536 - Psyr_1376 0.27 +3.0 1,559,536 - Psyr_1376 0.27 -0.0 1,559,536 - Psyr_1376 0.27 -0.4 1,559,628 - Psyr_1376 0.31 -4.0 1,559,770 + Psyr_1376 0.36 +0.1 1,559,771 - Psyr_1376 0.36 +1.3 1,559,821 - Psyr_1376 0.38 +2.7 1,559,934 - Psyr_1376 0.43 -0.8 1,559,934 - Psyr_1376 0.43 -1.0 1,559,934 - Psyr_1376 0.43 +1.3 1,559,934 - Psyr_1376 0.43 -1.3 1,559,934 - Psyr_1376 0.43 +2.7 1,559,934 - Psyr_1376 0.43 -0.9 1,559,937 + Psyr_1376 0.43 +0.5 1,559,937 + Psyr_1376 0.43 +1.5 1,559,937 + Psyr_1376 0.43 +1.6 1,559,937 + Psyr_1376 0.43 +0.6 1,559,937 + Psyr_1376 0.43 +1.5 1,559,937 + Psyr_1376 0.43 -0.6 1,559,938 - Psyr_1376 0.43 -1.2 1,559,938 - Psyr_1376 0.43 +0.7 1,559,938 - Psyr_1376 0.43 -3.7 1,559,938 - Psyr_1376 0.43 -1.6 1,559,986 - Psyr_1376 0.45 -0.2 1,559,986 - Psyr_1376 0.45 -2.9 1,560,204 + Psyr_1376 0.53 -3.1 1,560,205 - Psyr_1376 0.53 +4.0 1,560,205 - Psyr_1376 0.53 -1.6 1,560,644 + Psyr_1376 0.70 -1.1 1,560,645 - Psyr_1376 0.70 -3.8 1,560,681 + Psyr_1376 0.72 +3.7 1,560,681 + Psyr_1376 0.72 +2.0 1,560,681 + Psyr_1376 0.72 +0.4 1,560,968 + Psyr_1376 0.83 -2.6 1,561,173 + -1.2 1,561,191 + -1.1 1,561,250 + -0.8 1,561,250 + +4.2 1,561,250 + +0.4 1,561,251 - -0.7 1,561,288 - -0.6 1,561,300 - -0.4 1,561,500 + +0.4 1,561,501 - +0.7 1,561,547 + -1.2 1,561,548 - +0.3 1,561,565 + -0.6 1,561,614 + +3.2 1,561,614 + -0.2 1,561,614 + -0.2 1,561,614 + -1.9 1,561,654 + +0.4 1,561,654 + +0.4 1,561,655 - +0.3 1,561,655 - +4.0 1,561,670 + -2.9 1,561,670 + -0.6 1,561,671 - -1.2 1,561,672 + +0.7 1,561,672 + -0.4 1,561,799 + -0.3 1,561,799 + +1.4 1,561,799 + -1.6 1,561,799 + +0.8 1,561,799 + -3.4 1,561,800 - +0.4 1,561,800 - -1.9 1,561,800 - +2.0 1,561,969 + Psyr_1377 0.28 -0.0 1,562,045 + Psyr_1377 0.41 -1.8 1,562,046 - Psyr_1377 0.41 +2.6 1,562,088 + Psyr_1377 0.48 -0.3 1,562,088 + Psyr_1377 0.48 -0.3 1,562,088 + Psyr_1377 0.48 +1.6 1,562,089 - Psyr_1377 0.49 -0.1 1,562,089 - Psyr_1377 0.49 +3.6 1,562,089 - Psyr_1377 0.49 -0.7 1,562,350 - +0.7 1,562,410 - +0.4
Or see this region's nucleotide sequence