Strain Fitness in Pseudomonas syringae pv. syringae B728a ΔmexB around Psyr_1102

Experiment: KB with Acridine orange 3.125 ug/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_1101 and Psyr_1102 are separated by 695 nucleotidesPsyr_1102 and Psyr_1103 are separated by 98 nucleotidesPsyr_1103 and Psyr_1104 overlap by 4 nucleotides Psyr_1101: Psyr_1101 - Helix-turn-helix, Fis-type, at 1,246,586 to 1,248,094 _1101 Psyr_1102: Psyr_1102 - conserved hypothetical protein, at 1,248,790 to 1,249,146 _1102 Psyr_1103: Psyr_1103 - Protein of unknown function DUF454, at 1,249,245 to 1,249,703 _1103 Psyr_1104: Psyr_1104 - Heme oxygenase, at 1,249,700 to 1,250,314 _1104 Position (kb) 1248 1249 1250Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4 5at 1247.979 kb on + strandat 1247.979 kb on + strandat 1247.979 kb on + strandat 1248.131 kb on + strandat 1248.131 kb on + strandat 1248.131 kb on + strandat 1248.131 kb on + strandat 1248.131 kb on + strandat 1248.131 kb on + strandat 1248.131 kb on + strandat 1248.132 kb on - strandat 1248.132 kb on - strandat 1248.132 kb on - strandat 1248.132 kb on - strandat 1248.132 kb on - strandat 1248.132 kb on - strandat 1248.212 kb on + strandat 1248.213 kb on - strandat 1248.213 kb on - strandat 1248.213 kb on - strandat 1248.399 kb on + strandat 1248.458 kb on - strandat 1248.506 kb on + strandat 1248.696 kb on + strandat 1248.869 kb on + strand, within Psyr_1102at 1248.869 kb on + strand, within Psyr_1102at 1248.870 kb on - strand, within Psyr_1102at 1249.068 kb on - strand, within Psyr_1102at 1249.068 kb on - strand, within Psyr_1102at 1249.085 kb on + strand, within Psyr_1102at 1249.086 kb on - strand, within Psyr_1102at 1249.086 kb on - strand, within Psyr_1102at 1249.086 kb on - strand, within Psyr_1102at 1249.086 kb on - strand, within Psyr_1102at 1249.086 kb on - strand, within Psyr_1102at 1249.086 kb on - strand, within Psyr_1102at 1249.142 kb on + strandat 1249.142 kb on + strandat 1249.143 kb on - strandat 1249.143 kb on - strandat 1249.143 kb on - strandat 1249.255 kb on - strandat 1249.255 kb on - strandat 1249.255 kb on - strandat 1249.287 kb on + strandat 1249.287 kb on + strandat 1249.287 kb on + strandat 1249.287 kb on + strandat 1249.288 kb on - strandat 1249.321 kb on + strand, within Psyr_1103at 1249.322 kb on - strand, within Psyr_1103at 1249.322 kb on - strand, within Psyr_1103at 1249.435 kb on + strand, within Psyr_1103at 1249.435 kb on + strand, within Psyr_1103at 1249.435 kb on + strand, within Psyr_1103at 1249.435 kb on + strand, within Psyr_1103at 1249.435 kb on + strand, within Psyr_1103at 1249.436 kb on - strand, within Psyr_1103at 1249.436 kb on - strand, within Psyr_1103at 1249.436 kb on - strand, within Psyr_1103at 1249.436 kb on - strand, within Psyr_1103at 1249.569 kb on + strand, within Psyr_1103at 1249.602 kb on + strand, within Psyr_1103at 1249.602 kb on + strand, within Psyr_1103at 1249.602 kb on + strand, within Psyr_1103at 1249.602 kb on + strand, within Psyr_1103at 1249.602 kb on + strand, within Psyr_1103at 1249.603 kb on - strand, within Psyr_1103at 1249.603 kb on - strand, within Psyr_1103at 1249.603 kb on - strand, within Psyr_1103at 1249.636 kb on + strand, within Psyr_1103at 1249.636 kb on + strand, within Psyr_1103at 1249.636 kb on + strand, within Psyr_1103at 1249.636 kb on + strand, within Psyr_1103at 1249.636 kb on + strand, within Psyr_1103at 1249.636 kb on + strand, within Psyr_1103at 1249.636 kb on + strand, within Psyr_1103at 1249.637 kb on - strand, within Psyr_1103at 1249.637 kb on - strand, within Psyr_1103at 1250.136 kb on + strand, within Psyr_1104at 1250.136 kb on + strand, within Psyr_1104

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Acridine orange 3.125 ug/ml
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1,247,979 + +4.0
1,247,979 + +3.2
1,247,979 + -1.2
1,248,131 + -2.4
1,248,131 + +0.5
1,248,131 + +1.5
1,248,131 + -3.6
1,248,131 + -1.4
1,248,131 + +1.1
1,248,131 + -2.1
1,248,132 - -0.4
1,248,132 - -1.6
1,248,132 - -0.1
1,248,132 - +4.7
1,248,132 - +4.2
1,248,132 - -0.6
1,248,212 + -0.0
1,248,213 - +0.4
1,248,213 - +0.3
1,248,213 - -0.9
1,248,399 + -0.5
1,248,458 - -3.8
1,248,506 + -2.2
1,248,696 + +0.4
1,248,869 + Psyr_1102 0.22 -0.5
1,248,869 + Psyr_1102 0.22 +3.0
1,248,870 - Psyr_1102 0.22 -0.7
1,249,068 - Psyr_1102 0.78 -2.4
1,249,068 - Psyr_1102 0.78 -1.5
1,249,085 + Psyr_1102 0.83 -0.1
1,249,086 - Psyr_1102 0.83 +3.7
1,249,086 - Psyr_1102 0.83 +2.7
1,249,086 - Psyr_1102 0.83 -2.8
1,249,086 - Psyr_1102 0.83 -0.1
1,249,086 - Psyr_1102 0.83 -0.4
1,249,086 - Psyr_1102 0.83 +0.2
1,249,142 + -2.5
1,249,142 + +1.6
1,249,143 - -3.5
1,249,143 - +4.0
1,249,143 - -3.4
1,249,255 - +0.4
1,249,255 - +0.3
1,249,255 - -0.9
1,249,287 + -1.2
1,249,287 + +0.4
1,249,287 + -1.4
1,249,287 + +3.7
1,249,288 - -2.9
1,249,321 + Psyr_1103 0.17 +2.7
1,249,322 - Psyr_1103 0.17 -0.3
1,249,322 - Psyr_1103 0.17 -2.6
1,249,435 + Psyr_1103 0.41 -0.4
1,249,435 + Psyr_1103 0.41 +1.4
1,249,435 + Psyr_1103 0.41 +0.4
1,249,435 + Psyr_1103 0.41 +0.4
1,249,435 + Psyr_1103 0.41 +1.2
1,249,436 - Psyr_1103 0.42 +3.0
1,249,436 - Psyr_1103 0.42 +0.1
1,249,436 - Psyr_1103 0.42 +3.2
1,249,436 - Psyr_1103 0.42 +2.7
1,249,569 + Psyr_1103 0.71 +3.0
1,249,602 + Psyr_1103 0.78 +3.0
1,249,602 + Psyr_1103 0.78 +0.3
1,249,602 + Psyr_1103 0.78 -1.1
1,249,602 + Psyr_1103 0.78 +3.4
1,249,602 + Psyr_1103 0.78 -0.2
1,249,603 - Psyr_1103 0.78 -0.8
1,249,603 - Psyr_1103 0.78 +3.2
1,249,603 - Psyr_1103 0.78 +0.1
1,249,636 + Psyr_1103 0.85 -1.0
1,249,636 + Psyr_1103 0.85 +1.0
1,249,636 + Psyr_1103 0.85 +5.2
1,249,636 + Psyr_1103 0.85 +1.7
1,249,636 + Psyr_1103 0.85 +3.2
1,249,636 + Psyr_1103 0.85 -2.4
1,249,636 + Psyr_1103 0.85 +1.7
1,249,637 - Psyr_1103 0.85 +0.4
1,249,637 - Psyr_1103 0.85 +0.4
1,250,136 + Psyr_1104 0.71 -1.3
1,250,136 + Psyr_1104 0.71 +0.5

Or see this region's nucleotide sequence