Experiment: KB with Acridine orange 3.125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0826 and Psyr_0827 are separated by 253 nucleotides Psyr_0827 and Psyr_0828 overlap by 4 nucleotides
Psyr_0826: Psyr_0826 - glucose-6-phosphate isomerase, at 934,924 to 936,588
_0826
Psyr_0827: Psyr_0827 - pantothenate synthetase, at 936,842 to 937,693
_0827
Psyr_0828: Psyr_0828 - ketopantoate hydroxymethyltransferase, at 937,690 to 938,490
_0828
Position (kb)
936
937
938 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 935.943 kb on + strand, within Psyr_0826 at 935.943 kb on + strand, within Psyr_0826 at 936.038 kb on + strand, within Psyr_0826 at 936.220 kb on + strand, within Psyr_0826 at 936.220 kb on + strand, within Psyr_0826 at 936.221 kb on - strand, within Psyr_0826 at 936.221 kb on - strand, within Psyr_0826 at 936.332 kb on + strand, within Psyr_0826 at 936.332 kb on + strand, within Psyr_0826 at 936.333 kb on - strand, within Psyr_0826 at 936.333 kb on - strand, within Psyr_0826 at 936.333 kb on - strand, within Psyr_0826 at 936.578 kb on + strand at 936.579 kb on - strand at 936.581 kb on + strand at 936.661 kb on + strand at 936.662 kb on - strand at 936.687 kb on - strand at 936.710 kb on + strand at 936.710 kb on + strand at 936.710 kb on + strand at 936.710 kb on + strand at 936.711 kb on - strand at 936.711 kb on - strand at 936.711 kb on - strand at 936.711 kb on - strand at 936.711 kb on - strand at 936.762 kb on + strand at 936.763 kb on - strand at 936.797 kb on - strand at 936.817 kb on + strand at 936.817 kb on + strand at 936.817 kb on + strand at 936.817 kb on + strand at 936.818 kb on - strand at 936.818 kb on - strand at 936.818 kb on - strand at 936.959 kb on + strand, within Psyr_0827 at 936.959 kb on + strand, within Psyr_0827 at 936.969 kb on + strand, within Psyr_0827 at 937.080 kb on + strand, within Psyr_0827 at 937.080 kb on + strand, within Psyr_0827 at 937.081 kb on - strand, within Psyr_0827 at 937.119 kb on + strand, within Psyr_0827 at 937.482 kb on + strand, within Psyr_0827 at 937.531 kb on + strand, within Psyr_0827 at 937.531 kb on + strand, within Psyr_0827 at 937.532 kb on - strand, within Psyr_0827 at 937.682 kb on + strand at 937.683 kb on - strand at 937.748 kb on + strand at 937.748 kb on + strand at 937.749 kb on - strand at 937.749 kb on - strand at 937.749 kb on - strand at 938.292 kb on - strand, within Psyr_0828 at 938.310 kb on + strand, within Psyr_0828 at 938.363 kb on - strand, within Psyr_0828 at 938.418 kb on - strand at 938.472 kb on + strand at 938.492 kb on + strand at 938.492 kb on + strand at 938.492 kb on + strand at 938.492 kb on + strand at 938.492 kb on + strand at 938.492 kb on + strand at 938.492 kb on + strand at 938.492 kb on + strand at 938.492 kb on + strand at 938.492 kb on + strand at 938.492 kb on + strand at 938.492 kb on + strand at 938.492 kb on + strand at 938.493 kb on - strand at 938.493 kb on - strand at 938.493 kb on - strand at 938.493 kb on - strand at 938.493 kb on - strand at 938.493 kb on - strand at 938.493 kb on - strand at 938.493 kb on - strand at 938.493 kb on - strand at 938.493 kb on - strand at 938.493 kb on - strand at 938.493 kb on - strand at 938.493 kb on - strand at 938.493 kb on - strand at 938.493 kb on - strand at 938.633 kb on + strand at 938.633 kb on + strand at 938.634 kb on - strand at 938.673 kb on + strand at 938.673 kb on + strand at 938.673 kb on + strand at 938.673 kb on + strand at 938.674 kb on - strand at 938.674 kb on - strand at 938.693 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 3.125 ug/ml remove 935,943 + Psyr_0826 0.61 +0.5 935,943 + Psyr_0826 0.61 +0.5 936,038 + Psyr_0826 0.67 +0.5 936,220 + Psyr_0826 0.78 -2.7 936,220 + Psyr_0826 0.78 +0.5 936,221 - Psyr_0826 0.78 +2.5 936,221 - Psyr_0826 0.78 -3.2 936,332 + Psyr_0826 0.85 +0.5 936,332 + Psyr_0826 0.85 -2.6 936,333 - Psyr_0826 0.85 -2.7 936,333 - Psyr_0826 0.85 +0.5 936,333 - Psyr_0826 0.85 +4.0 936,578 + -4.2 936,579 - +0.5 936,581 + +1.5 936,661 + -3.8 936,662 - -0.5 936,687 - -1.8 936,710 + -2.3 936,710 + -2.1 936,710 + -0.5 936,710 + -1.5 936,711 - +2.5 936,711 - +0.5 936,711 - +0.3 936,711 - -1.0 936,711 - +1.5 936,762 + +1.5 936,763 - +0.7 936,797 - -1.7 936,817 + +0.1 936,817 + +3.5 936,817 + -2.5 936,817 + +0.6 936,818 - +1.7 936,818 - +0.8 936,818 - -0.3 936,959 + Psyr_0827 0.14 +0.5 936,959 + Psyr_0827 0.14 +2.5 936,969 + Psyr_0827 0.15 -0.5 937,080 + Psyr_0827 0.28 +0.2 937,080 + Psyr_0827 0.28 -0.6 937,081 - Psyr_0827 0.28 +0.2 937,119 + Psyr_0827 0.33 -4.2 937,482 + Psyr_0827 0.75 +1.4 937,531 + Psyr_0827 0.81 -1.9 937,531 + Psyr_0827 0.81 -2.9 937,532 - Psyr_0827 0.81 -2.0 937,682 + +0.5 937,683 - -3.2 937,748 + -2.1 937,748 + +0.2 937,749 - -1.5 937,749 - -0.4 937,749 - +0.0 938,292 - Psyr_0828 0.75 +1.1 938,310 + Psyr_0828 0.77 +2.8 938,363 - Psyr_0828 0.84 -0.3 938,418 - -2.5 938,472 + -3.2 938,492 + -1.0 938,492 + +3.3 938,492 + -1.1 938,492 + +3.9 938,492 + +0.7 938,492 + -4.5 938,492 + -0.0 938,492 + +0.7 938,492 + +3.0 938,492 + +4.3 938,492 + +0.2 938,492 + +0.5 938,492 + -0.2 938,493 - -1.1 938,493 - +3.3 938,493 - +0.5 938,493 - +0.6 938,493 - -1.4 938,493 - +4.0 938,493 - +2.0 938,493 - +0.3 938,493 - -3.3 938,493 - -1.2 938,493 - +1.4 938,493 - -2.1 938,493 - -0.4 938,493 - -2.1 938,493 - +1.5 938,633 + +1.8 938,633 + +1.4 938,634 - +3.5 938,673 + -1.9 938,673 + +2.3 938,673 + -2.5 938,673 + +0.5 938,674 - -0.1 938,674 - +3.8 938,693 + -0.4
Or see this region's nucleotide sequence