Experiment: KB with Acridine orange 3.125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0571 and Psyr_0572 are separated by 93 nucleotides Psyr_0572 and Psyr_0573 are separated by 12 nucleotides Psyr_0573 and Psyr_0574 are separated by 96 nucleotides
Psyr_0571: Psyr_0571 - tRNA isopentenyltransferase, at 649,554 to 650,525
_0571
Psyr_0572: Psyr_0572 - RNA-binding protein Hfq, at 650,619 to 650,879
_0572
Psyr_0573: Psyr_0573 - GTP-binding protein HflX, at 650,892 to 652,193
_0573
Psyr_0574: Psyr_0574 - protease FtsH subunit HflK, at 652,290 to 653,492
_0574
Position (kb) 650
651
652
653 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 650.015 kb on + strand, within Psyr_0571 at 650.015 kb on + strand, within Psyr_0571 at 650.016 kb on - strand, within Psyr_0571 at 650.016 kb on - strand, within Psyr_0571 at 650.016 kb on - strand, within Psyr_0571 at 650.154 kb on + strand, within Psyr_0571 at 650.155 kb on - strand, within Psyr_0571 at 650.157 kb on - strand, within Psyr_0571 at 650.157 kb on - strand, within Psyr_0571 at 650.157 kb on - strand, within Psyr_0571 at 650.515 kb on + strand at 650.515 kb on + strand at 650.544 kb on + strand at 650.593 kb on + strand at 650.603 kb on + strand at 650.871 kb on - strand at 650.880 kb on + strand at 651.060 kb on + strand, within Psyr_0573 at 651.061 kb on - strand, within Psyr_0573 at 651.061 kb on - strand, within Psyr_0573 at 651.061 kb on - strand, within Psyr_0573 at 651.242 kb on + strand, within Psyr_0573 at 651.840 kb on + strand, within Psyr_0573 at 651.840 kb on + strand, within Psyr_0573 at 651.840 kb on + strand, within Psyr_0573 at 651.840 kb on + strand, within Psyr_0573 at 651.840 kb on + strand, within Psyr_0573 at 651.840 kb on + strand, within Psyr_0573 at 651.841 kb on - strand, within Psyr_0573 at 651.868 kb on + strand, within Psyr_0573 at 651.880 kb on + strand, within Psyr_0573 at 651.881 kb on - strand, within Psyr_0573 at 652.191 kb on + strand at 652.213 kb on + strand at 652.213 kb on + strand at 652.213 kb on + strand at 652.213 kb on + strand at 652.235 kb on + strand at 652.236 kb on - strand at 652.398 kb on - strand at 652.549 kb on - strand, within Psyr_0574 at 652.556 kb on + strand, within Psyr_0574 at 652.556 kb on + strand, within Psyr_0574 at 652.560 kb on + strand, within Psyr_0574 at 652.560 kb on + strand, within Psyr_0574 at 652.560 kb on + strand, within Psyr_0574 at 652.560 kb on + strand, within Psyr_0574 at 652.560 kb on + strand, within Psyr_0574 at 652.560 kb on + strand, within Psyr_0574 at 652.641 kb on + strand, within Psyr_0574 at 652.665 kb on + strand, within Psyr_0574 at 652.761 kb on + strand, within Psyr_0574
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 3.125 ug/ml remove 650,015 + Psyr_0571 0.47 -0.8 650,015 + Psyr_0571 0.47 +0.3 650,016 - Psyr_0571 0.48 +1.3 650,016 - Psyr_0571 0.48 -0.6 650,016 - Psyr_0571 0.48 -0.2 650,154 + Psyr_0571 0.62 +1.1 650,155 - Psyr_0571 0.62 +0.5 650,157 - Psyr_0571 0.62 -2.3 650,157 - Psyr_0571 0.62 +0.5 650,157 - Psyr_0571 0.62 -1.5 650,515 + -1.2 650,515 + +0.1 650,544 + +0.2 650,593 + -0.5 650,603 + -1.1 650,871 - +0.5 650,880 + +0.1 651,060 + Psyr_0573 0.13 +0.5 651,061 - Psyr_0573 0.13 +0.5 651,061 - Psyr_0573 0.13 +0.5 651,061 - Psyr_0573 0.13 -1.8 651,242 + Psyr_0573 0.27 +1.5 651,840 + Psyr_0573 0.73 +3.1 651,840 + Psyr_0573 0.73 -0.7 651,840 + Psyr_0573 0.73 -1.6 651,840 + Psyr_0573 0.73 -0.7 651,840 + Psyr_0573 0.73 -1.0 651,840 + Psyr_0573 0.73 +0.3 651,841 - Psyr_0573 0.73 +0.5 651,868 + Psyr_0573 0.75 +0.5 651,880 + Psyr_0573 0.76 -0.9 651,881 - Psyr_0573 0.76 +0.5 652,191 + +0.1 652,213 + -2.5 652,213 + -4.1 652,213 + +0.5 652,213 + -0.3 652,235 + +1.3 652,236 - -1.2 652,398 - +0.5 652,549 - Psyr_0574 0.22 -2.3 652,556 + Psyr_0574 0.22 +0.5 652,556 + Psyr_0574 0.22 +0.5 652,560 + Psyr_0574 0.22 -2.1 652,560 + Psyr_0574 0.22 +0.5 652,560 + Psyr_0574 0.22 -1.3 652,560 + Psyr_0574 0.22 +0.4 652,560 + Psyr_0574 0.22 -2.9 652,560 + Psyr_0574 0.22 -3.6 652,641 + Psyr_0574 0.29 -3.2 652,665 + Psyr_0574 0.31 -0.3 652,761 + Psyr_0574 0.39 +3.1
Or see this region's nucleotide sequence