Experiment: KB with Acridine orange 3.125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0540 and Psyr_0541 are separated by 48 nucleotides Psyr_0541 and Psyr_0542 are separated by 204 nucleotides
Psyr_0540: Psyr_0540 - oxidoreductase, FAD-binding protein, at 610,174 to 611,349
_0540
Psyr_0541: Psyr_0541 - Small multidrug resistance protein, at 611,398 to 611,730
_0541
Psyr_0542: Psyr_0542 - Three-deoxy-D-manno-octulosonic-acid transferase, N-terminal, at 611,935 to 613,080
_0542
Position (kb) 611
612 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 611.382 kb on - strand at 611.382 kb on - strand at 611.382 kb on - strand at 611.382 kb on - strand at 611.382 kb on - strand at 611.383 kb on + strand at 611.383 kb on + strand at 611.384 kb on - strand at 611.384 kb on - strand at 611.384 kb on - strand at 611.496 kb on - strand, within Psyr_0541 at 611.552 kb on + strand, within Psyr_0541 at 611.552 kb on + strand, within Psyr_0541 at 611.552 kb on + strand, within Psyr_0541 at 611.552 kb on + strand, within Psyr_0541 at 611.552 kb on + strand, within Psyr_0541 at 611.553 kb on - strand, within Psyr_0541 at 611.553 kb on - strand, within Psyr_0541 at 611.553 kb on - strand, within Psyr_0541 at 611.553 kb on - strand, within Psyr_0541 at 611.553 kb on - strand, within Psyr_0541 at 611.553 kb on - strand, within Psyr_0541 at 611.717 kb on + strand at 611.717 kb on + strand at 611.717 kb on + strand at 611.718 kb on - strand at 611.718 kb on - strand at 611.718 kb on - strand at 611.718 kb on - strand at 611.720 kb on + strand at 611.720 kb on + strand at 611.762 kb on + strand at 611.763 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 3.125 ug/ml remove 611,382 - +2.6 611,382 - +0.9 611,382 - +1.6 611,382 - +0.5 611,382 - +0.4 611,383 + -2.1 611,383 + +0.5 611,384 - -0.8 611,384 - -2.2 611,384 - +1.4 611,496 - Psyr_0541 0.29 +1.0 611,552 + Psyr_0541 0.46 -0.9 611,552 + Psyr_0541 0.46 -1.6 611,552 + Psyr_0541 0.46 -1.6 611,552 + Psyr_0541 0.46 -0.4 611,552 + Psyr_0541 0.46 +1.2 611,553 - Psyr_0541 0.47 -1.8 611,553 - Psyr_0541 0.47 -1.3 611,553 - Psyr_0541 0.47 -0.7 611,553 - Psyr_0541 0.47 +4.0 611,553 - Psyr_0541 0.47 -1.1 611,553 - Psyr_0541 0.47 -2.1 611,717 + -3.1 611,717 + -1.7 611,717 + +3.3 611,718 - -0.7 611,718 - -0.3 611,718 - +0.2 611,718 - -2.1 611,720 + -0.1 611,720 + -0.6 611,762 + +0.5 611,763 - +0.5
Or see this region's nucleotide sequence