Experiment: KB with Acridine orange 3.125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0500 and Psyr_0501 are separated by 58 nucleotides Psyr_0501 and Psyr_0502 are separated by 23 nucleotides
Psyr_0500: Psyr_0500 - biotin carboxyl carrier protein / biotin carboxylase, at 542,495 to 544,228
_0500
Psyr_0501: Psyr_0501 - MscS Mechanosensitive ion channel, at 544,287 to 547,691
_0501
Psyr_0502: Psyr_0502 - potassium/proton antiporter, CPA1 family, at 547,715 to 549,511
_0502
Position (kb)
544
545
546
547
548 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 543.293 kb on + strand, within Psyr_0500 at 543.550 kb on + strand, within Psyr_0500 at 543.551 kb on - strand, within Psyr_0500 at 543.558 kb on + strand, within Psyr_0500 at 543.558 kb on + strand, within Psyr_0500 at 543.558 kb on + strand, within Psyr_0500 at 543.559 kb on - strand, within Psyr_0500 at 543.793 kb on + strand, within Psyr_0500 at 543.793 kb on + strand, within Psyr_0500 at 543.794 kb on - strand, within Psyr_0500 at 544.187 kb on + strand at 544.188 kb on - strand at 544.420 kb on + strand at 544.420 kb on + strand at 544.420 kb on + strand at 544.420 kb on + strand at 544.421 kb on - strand at 544.447 kb on + strand at 544.447 kb on + strand at 544.447 kb on + strand at 544.448 kb on - strand at 544.448 kb on - strand at 544.448 kb on - strand at 544.554 kb on + strand at 544.554 kb on + strand at 544.554 kb on + strand at 544.606 kb on + strand at 544.607 kb on - strand at 544.607 kb on - strand at 544.607 kb on - strand at 544.659 kb on + strand, within Psyr_0501 at 544.659 kb on + strand, within Psyr_0501 at 544.659 kb on + strand, within Psyr_0501 at 545.057 kb on - strand, within Psyr_0501 at 545.121 kb on + strand, within Psyr_0501 at 545.122 kb on - strand, within Psyr_0501 at 545.122 kb on - strand, within Psyr_0501 at 545.122 kb on - strand, within Psyr_0501 at 545.245 kb on + strand, within Psyr_0501 at 545.245 kb on + strand, within Psyr_0501 at 545.245 kb on + strand, within Psyr_0501 at 545.245 kb on + strand, within Psyr_0501 at 545.246 kb on - strand, within Psyr_0501 at 545.246 kb on - strand, within Psyr_0501 at 545.246 kb on - strand, within Psyr_0501 at 545.308 kb on + strand, within Psyr_0501 at 545.308 kb on + strand, within Psyr_0501 at 545.309 kb on - strand, within Psyr_0501 at 545.309 kb on - strand, within Psyr_0501 at 545.462 kb on - strand, within Psyr_0501 at 545.536 kb on + strand, within Psyr_0501 at 545.537 kb on - strand, within Psyr_0501 at 545.578 kb on + strand, within Psyr_0501 at 545.578 kb on + strand, within Psyr_0501 at 545.579 kb on - strand, within Psyr_0501 at 545.579 kb on - strand, within Psyr_0501 at 545.579 kb on - strand, within Psyr_0501 at 545.579 kb on - strand, within Psyr_0501 at 545.579 kb on - strand, within Psyr_0501 at 545.581 kb on + strand, within Psyr_0501 at 545.581 kb on + strand, within Psyr_0501 at 545.581 kb on + strand, within Psyr_0501 at 545.581 kb on + strand, within Psyr_0501 at 545.581 kb on + strand, within Psyr_0501 at 545.581 kb on + strand, within Psyr_0501 at 545.581 kb on + strand, within Psyr_0501 at 545.581 kb on + strand, within Psyr_0501 at 545.581 kb on + strand, within Psyr_0501 at 545.582 kb on - strand, within Psyr_0501 at 545.582 kb on - strand, within Psyr_0501 at 545.582 kb on - strand, within Psyr_0501 at 545.582 kb on - strand, within Psyr_0501 at 545.582 kb on - strand, within Psyr_0501 at 545.585 kb on - strand, within Psyr_0501 at 545.585 kb on - strand, within Psyr_0501 at 545.926 kb on + strand, within Psyr_0501 at 545.926 kb on + strand, within Psyr_0501 at 545.926 kb on + strand, within Psyr_0501 at 545.926 kb on + strand, within Psyr_0501 at 545.927 kb on - strand, within Psyr_0501 at 545.927 kb on - strand, within Psyr_0501 at 545.927 kb on - strand, within Psyr_0501 at 546.168 kb on + strand, within Psyr_0501 at 546.290 kb on + strand, within Psyr_0501 at 546.290 kb on + strand, within Psyr_0501 at 546.553 kb on + strand, within Psyr_0501 at 546.554 kb on - strand, within Psyr_0501 at 546.605 kb on - strand, within Psyr_0501 at 546.679 kb on + strand, within Psyr_0501 at 546.680 kb on - strand, within Psyr_0501 at 546.830 kb on - strand, within Psyr_0501 at 547.676 kb on + strand at 547.676 kb on + strand at 547.697 kb on + strand at 547.697 kb on + strand at 547.697 kb on + strand at 547.697 kb on + strand at 547.698 kb on - strand at 547.698 kb on - strand at 547.698 kb on - strand at 547.698 kb on - strand at 547.717 kb on - strand at 547.810 kb on + strand at 547.810 kb on + strand at 547.810 kb on + strand at 547.810 kb on + strand at 547.811 kb on - strand at 547.811 kb on - strand at 547.811 kb on - strand at 547.811 kb on - strand at 547.811 kb on - strand at 547.921 kb on - strand, within Psyr_0502 at 547.921 kb on - strand, within Psyr_0502 at 547.921 kb on - strand, within Psyr_0502 at 548.087 kb on - strand, within Psyr_0502 at 548.087 kb on - strand, within Psyr_0502 at 548.237 kb on + strand, within Psyr_0502 at 548.237 kb on + strand, within Psyr_0502 at 548.238 kb on - strand, within Psyr_0502 at 548.238 kb on - strand, within Psyr_0502 at 548.238 kb on - strand, within Psyr_0502 at 548.238 kb on - strand, within Psyr_0502 at 548.238 kb on - strand, within Psyr_0502 at 548.370 kb on + strand, within Psyr_0502 at 548.370 kb on + strand, within Psyr_0502 at 548.371 kb on - strand, within Psyr_0502 at 548.371 kb on - strand, within Psyr_0502 at 548.371 kb on - strand, within Psyr_0502 at 548.371 kb on - strand, within Psyr_0502 at 548.371 kb on - strand, within Psyr_0502
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 3.125 ug/ml remove 543,293 + Psyr_0500 0.46 +4.7 543,550 + Psyr_0500 0.61 +0.5 543,551 - Psyr_0500 0.61 +1.3 543,558 + Psyr_0500 0.61 -2.1 543,558 + Psyr_0500 0.61 -3.3 543,558 + Psyr_0500 0.61 -0.1 543,559 - Psyr_0500 0.61 -2.3 543,793 + Psyr_0500 0.75 -0.7 543,793 + Psyr_0500 0.75 -1.3 543,794 - Psyr_0500 0.75 -0.1 544,187 + +4.6 544,188 - +0.9 544,420 + -2.8 544,420 + +0.4 544,420 + +0.2 544,420 + +0.2 544,421 - -0.5 544,447 + +2.9 544,447 + -0.6 544,447 + +0.7 544,448 - +1.5 544,448 - +0.2 544,448 - +1.0 544,554 + -1.8 544,554 + -0.2 544,554 + +0.5 544,606 + +4.1 544,607 - -4.2 544,607 - +0.3 544,607 - -4.4 544,659 + Psyr_0501 0.11 -0.7 544,659 + Psyr_0501 0.11 +2.2 544,659 + Psyr_0501 0.11 -0.5 545,057 - Psyr_0501 0.23 -0.6 545,121 + Psyr_0501 0.24 +2.5 545,122 - Psyr_0501 0.25 -2.4 545,122 - Psyr_0501 0.25 -1.4 545,122 - Psyr_0501 0.25 -0.9 545,245 + Psyr_0501 0.28 -2.3 545,245 + Psyr_0501 0.28 +5.7 545,245 + Psyr_0501 0.28 -0.5 545,245 + Psyr_0501 0.28 -2.0 545,246 - Psyr_0501 0.28 -1.3 545,246 - Psyr_0501 0.28 +2.2 545,246 - Psyr_0501 0.28 -1.5 545,308 + Psyr_0501 0.30 -0.5 545,308 + Psyr_0501 0.30 -0.4 545,309 - Psyr_0501 0.30 +0.7 545,309 - Psyr_0501 0.30 +4.9 545,462 - Psyr_0501 0.35 -1.9 545,536 + Psyr_0501 0.37 +0.0 545,537 - Psyr_0501 0.37 -0.4 545,578 + Psyr_0501 0.38 +4.2 545,578 + Psyr_0501 0.38 -0.7 545,579 - Psyr_0501 0.38 -1.3 545,579 - Psyr_0501 0.38 -1.8 545,579 - Psyr_0501 0.38 -0.3 545,579 - Psyr_0501 0.38 +0.5 545,579 - Psyr_0501 0.38 -0.5 545,581 + Psyr_0501 0.38 +4.1 545,581 + Psyr_0501 0.38 -0.3 545,581 + Psyr_0501 0.38 +1.8 545,581 + Psyr_0501 0.38 -1.0 545,581 + Psyr_0501 0.38 -0.6 545,581 + Psyr_0501 0.38 -0.2 545,581 + Psyr_0501 0.38 +0.1 545,581 + Psyr_0501 0.38 -3.9 545,581 + Psyr_0501 0.38 -0.1 545,582 - Psyr_0501 0.38 +0.5 545,582 - Psyr_0501 0.38 +0.7 545,582 - Psyr_0501 0.38 +0.4 545,582 - Psyr_0501 0.38 -0.7 545,582 - Psyr_0501 0.38 +4.0 545,585 - Psyr_0501 0.38 -1.7 545,585 - Psyr_0501 0.38 +4.8 545,926 + Psyr_0501 0.48 +1.9 545,926 + Psyr_0501 0.48 +1.4 545,926 + Psyr_0501 0.48 +0.7 545,926 + Psyr_0501 0.48 -0.9 545,927 - Psyr_0501 0.48 -0.3 545,927 - Psyr_0501 0.48 +0.0 545,927 - Psyr_0501 0.48 -2.2 546,168 + Psyr_0501 0.55 -1.9 546,290 + Psyr_0501 0.59 +3.5 546,290 + Psyr_0501 0.59 -0.6 546,553 + Psyr_0501 0.67 -0.5 546,554 - Psyr_0501 0.67 +1.1 546,605 - Psyr_0501 0.68 +0.1 546,679 + Psyr_0501 0.70 -0.6 546,680 - Psyr_0501 0.70 +3.7 546,830 - Psyr_0501 0.75 -1.5 547,676 + -1.1 547,676 + +0.1 547,697 + -0.6 547,697 + -0.3 547,697 + -0.2 547,697 + -1.9 547,698 - +0.5 547,698 - -2.0 547,698 - +0.2 547,698 - +3.3 547,717 - -0.9 547,810 + +3.1 547,810 + +0.5 547,810 + +0.8 547,810 + -0.3 547,811 - +4.0 547,811 - +1.7 547,811 - -1.0 547,811 - -0.2 547,811 - +4.9 547,921 - Psyr_0502 0.11 -0.2 547,921 - Psyr_0502 0.11 -1.5 547,921 - Psyr_0502 0.11 +4.0 548,087 - Psyr_0502 0.21 +0.5 548,087 - Psyr_0502 0.21 -1.3 548,237 + Psyr_0502 0.29 -0.4 548,237 + Psyr_0502 0.29 -0.8 548,238 - Psyr_0502 0.29 +4.7 548,238 - Psyr_0502 0.29 -2.4 548,238 - Psyr_0502 0.29 +0.4 548,238 - Psyr_0502 0.29 +0.3 548,238 - Psyr_0502 0.29 +2.1 548,370 + Psyr_0502 0.36 +3.3 548,370 + Psyr_0502 0.36 +1.0 548,371 - Psyr_0502 0.37 +2.4 548,371 - Psyr_0502 0.37 +0.5 548,371 - Psyr_0502 0.37 -0.3 548,371 - Psyr_0502 0.37 +1.0 548,371 - Psyr_0502 0.37 -0.9
Or see this region's nucleotide sequence