Experiment: KB with Acridine orange 3.125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0291 and Psyr_0292 are separated by 231 nucleotides Psyr_0292 and Psyr_0293 are separated by 16 nucleotides
Psyr_0291: Psyr_0291 - DedA, at 309,519 to 310,181
_0291
Psyr_0292: Psyr_0292 - porphobilinogen synthase, at 310,413 to 311,423
_0292
Psyr_0293: Psyr_0293 - Polyphosphate kinase, at 311,440 to 313,650
_0293
Position (kb) 310
311
312 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3
4 at 309.681 kb on + strand, within Psyr_0291 at 309.681 kb on + strand, within Psyr_0291 at 309.709 kb on + strand, within Psyr_0291 at 309.709 kb on + strand, within Psyr_0291 at 309.710 kb on - strand, within Psyr_0291 at 309.818 kb on - strand, within Psyr_0291 at 309.878 kb on - strand, within Psyr_0291 at 310.006 kb on + strand, within Psyr_0291 at 310.006 kb on + strand, within Psyr_0291 at 310.006 kb on + strand, within Psyr_0291 at 310.006 kb on + strand, within Psyr_0291 at 310.007 kb on - strand, within Psyr_0291 at 310.007 kb on - strand, within Psyr_0291 at 310.007 kb on - strand, within Psyr_0291 at 310.027 kb on + strand, within Psyr_0291 at 310.072 kb on + strand, within Psyr_0291 at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.261 kb on + strand at 310.356 kb on + strand at 310.367 kb on + strand at 310.367 kb on + strand at 310.367 kb on + strand at 310.369 kb on + strand at 310.369 kb on + strand at 310.369 kb on + strand at 310.369 kb on + strand at 310.369 kb on + strand at 310.369 kb on + strand at 310.369 kb on + strand at 310.369 kb on + strand at 311.422 kb on - strand at 311.523 kb on - strand at 311.523 kb on - strand at 311.596 kb on + strand at 311.596 kb on + strand at 311.596 kb on + strand at 311.596 kb on + strand at 311.596 kb on + strand at 311.596 kb on + strand at 311.596 kb on + strand at 311.596 kb on + strand at 311.596 kb on + strand at 311.596 kb on + strand at 311.596 kb on + strand at 311.596 kb on + strand at 311.596 kb on + strand at 311.597 kb on - strand at 311.597 kb on - strand at 311.597 kb on - strand at 311.597 kb on - strand at 311.597 kb on - strand at 311.597 kb on - strand at 311.597 kb on - strand at 311.597 kb on - strand at 311.597 kb on - strand at 311.597 kb on - strand at 311.597 kb on - strand at 311.597 kb on - strand at 311.597 kb on - strand at 311.597 kb on - strand at 311.597 kb on - strand at 311.597 kb on - strand at 311.597 kb on - strand at 311.597 kb on - strand at 311.597 kb on - strand at 311.599 kb on - strand at 311.955 kb on - strand, within Psyr_0293 at 311.955 kb on - strand, within Psyr_0293 at 311.962 kb on + strand, within Psyr_0293 at 311.962 kb on + strand, within Psyr_0293 at 311.962 kb on + strand, within Psyr_0293 at 312.073 kb on - strand, within Psyr_0293 at 312.251 kb on - strand, within Psyr_0293
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 3.125 ug/ml remove 309,681 + Psyr_0291 0.24 -0.4 309,681 + Psyr_0291 0.24 +1.1 309,709 + Psyr_0291 0.29 +0.1 309,709 + Psyr_0291 0.29 -3.0 309,710 - Psyr_0291 0.29 -0.1 309,818 - Psyr_0291 0.45 +4.5 309,878 - Psyr_0291 0.54 -0.7 310,006 + Psyr_0291 0.73 +1.6 310,006 + Psyr_0291 0.73 +3.6 310,006 + Psyr_0291 0.73 +0.1 310,006 + Psyr_0291 0.73 -0.1 310,007 - Psyr_0291 0.74 -1.1 310,007 - Psyr_0291 0.74 -3.1 310,007 - Psyr_0291 0.74 +0.9 310,027 + Psyr_0291 0.77 +1.7 310,072 + Psyr_0291 0.83 +0.6 310,246 + -0.7 310,246 + -0.0 310,246 + -3.0 310,246 + -2.4 310,246 + +0.1 310,246 + -2.8 310,246 + -0.0 310,246 + +0.2 310,247 - -1.5 310,247 - -1.4 310,247 - -4.1 310,247 - -1.5 310,247 - -2.7 310,247 - -0.5 310,247 - +0.2 310,247 - -2.0 310,247 - +1.4 310,247 - -0.3 310,247 - +0.6 310,247 - -1.4 310,247 - +0.2 310,247 - -1.3 310,247 - +0.8 310,247 - +3.0 310,261 + -1.1 310,356 + +1.1 310,367 + -0.6 310,367 + +2.8 310,367 + -3.8 310,369 + -1.9 310,369 + +0.2 310,369 + +3.6 310,369 + +1.6 310,369 + -0.1 310,369 + -0.5 310,369 + +1.6 310,369 + +3.2 311,422 - -2.4 311,523 - +0.6 311,523 - -1.4 311,596 + -4.4 311,596 + -2.9 311,596 + -1.5 311,596 + -2.0 311,596 + -4.0 311,596 + +2.6 311,596 + -5.5 311,596 + -2.1 311,596 + -3.0 311,596 + -6.0 311,596 + -1.2 311,596 + -3.9 311,596 + -0.6 311,597 - +0.6 311,597 - -1.1 311,597 - -2.2 311,597 - -2.4 311,597 - -1.7 311,597 - +0.6 311,597 - -2.7 311,597 - -2.8 311,597 - -2.4 311,597 - -4.6 311,597 - -4.9 311,597 - -2.6 311,597 - -2.2 311,597 - -2.4 311,597 - -4.0 311,597 - -1.9 311,597 - -1.6 311,597 - -2.9 311,597 - -4.6 311,599 - -1.8 311,955 - Psyr_0293 0.23 -2.5 311,955 - Psyr_0293 0.23 +0.6 311,962 + Psyr_0293 0.24 -4.1 311,962 + Psyr_0293 0.24 -4.6 311,962 + Psyr_0293 0.24 -3.1 312,073 - Psyr_0293 0.29 -3.1 312,251 - Psyr_0293 0.37 -3.5
Or see this region's nucleotide sequence