Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_18225 and PS417_18230 are separated by 189 nucleotides PS417_18230 and PS417_18235 are separated by 73 nucleotides PS417_18235 and PS417_18240 are separated by 41 nucleotides
PS417_18225: PS417_18225 - phenol degradation protein meta, at 3,971,116 to 3,971,982
_18225
PS417_18230: PS417_18230 - amidase, at 3,972,172 to 3,973,686
_18230
PS417_18235: PS417_18235 - nitrile hydratase, at 3,973,760 to 3,974,353
_18235
PS417_18240: PS417_18240 - nitrile hydratase, at 3,974,395 to 3,975,057
_18240
Position (kb)
3972
3973
3974 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3971.183 kb on + strand at 3971.237 kb on - strand, within PS417_18225 at 3971.266 kb on + strand at 3971.524 kb on + strand, within PS417_18225 at 3971.524 kb on + strand at 3971.524 kb on + strand, within PS417_18225 at 3971.524 kb on + strand, within PS417_18225 at 3971.524 kb on + strand, within PS417_18225 at 3971.524 kb on + strand, within PS417_18225 at 3971.525 kb on - strand, within PS417_18225 at 3971.525 kb on - strand, within PS417_18225 at 3971.529 kb on - strand, within PS417_18225 at 3971.605 kb on + strand, within PS417_18225 at 3971.605 kb on + strand, within PS417_18225 at 3971.743 kb on + strand, within PS417_18225 at 3971.758 kb on - strand, within PS417_18225 at 3971.842 kb on - strand, within PS417_18225 at 3972.271 kb on - strand at 3972.333 kb on - strand, within PS417_18230 at 3972.394 kb on + strand at 3972.788 kb on - strand at 3972.801 kb on + strand, within PS417_18230 at 3972.801 kb on + strand, within PS417_18230 at 3972.820 kb on + strand, within PS417_18230 at 3972.820 kb on + strand, within PS417_18230 at 3972.964 kb on + strand, within PS417_18230 at 3972.964 kb on + strand, within PS417_18230 at 3972.965 kb on - strand, within PS417_18230 at 3972.965 kb on - strand, within PS417_18230 at 3973.228 kb on + strand, within PS417_18230 at 3973.229 kb on - strand, within PS417_18230 at 3973.336 kb on + strand, within PS417_18230 at 3973.393 kb on + strand, within PS417_18230 at 3973.489 kb on + strand, within PS417_18230 at 3973.490 kb on - strand, within PS417_18230 at 3973.618 kb on + strand at 3973.733 kb on - strand at 3973.932 kb on + strand, within PS417_18235 at 3974.016 kb on - strand, within PS417_18235 at 3974.241 kb on - strand, within PS417_18235 at 3974.457 kb on + strand at 3974.477 kb on + strand, within PS417_18240 at 3974.477 kb on + strand, within PS417_18240 at 3974.507 kb on - strand, within PS417_18240 at 3974.615 kb on - strand, within PS417_18240 at 3974.623 kb on + strand, within PS417_18240 at 3974.623 kb on + strand, within PS417_18240 at 3974.624 kb on - strand, within PS417_18240 at 3974.624 kb on - strand, within PS417_18240
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,971,183 + +0.0 3,971,237 - PS417_18225 0.14 +0.8 3,971,266 + +0.9 3,971,524 + PS417_18225 0.47 -0.1 3,971,524 + +1.9 3,971,524 + PS417_18225 0.47 +0.0 3,971,524 + PS417_18225 0.47 +0.6 3,971,524 + PS417_18225 0.47 -0.3 3,971,524 + PS417_18225 0.47 -0.5 3,971,525 - PS417_18225 0.47 +1.5 3,971,525 - PS417_18225 0.47 +0.6 3,971,529 - PS417_18225 0.48 -1.1 3,971,605 + PS417_18225 0.56 +1.7 3,971,605 + PS417_18225 0.56 +1.2 3,971,743 + PS417_18225 0.72 -0.4 3,971,758 - PS417_18225 0.74 -0.8 3,971,842 - PS417_18225 0.84 +1.3 3,972,271 - +0.5 3,972,333 - PS417_18230 0.11 +1.1 3,972,394 + +0.9 3,972,788 - -1.1 3,972,801 + PS417_18230 0.42 +0.9 3,972,801 + PS417_18230 0.42 +0.2 3,972,820 + PS417_18230 0.43 +0.5 3,972,820 + PS417_18230 0.43 -1.4 3,972,964 + PS417_18230 0.52 -0.0 3,972,964 + PS417_18230 0.52 +0.6 3,972,965 - PS417_18230 0.52 +0.3 3,972,965 - PS417_18230 0.52 +1.2 3,973,228 + PS417_18230 0.70 -2.1 3,973,229 - PS417_18230 0.70 +0.4 3,973,336 + PS417_18230 0.77 +1.2 3,973,393 + PS417_18230 0.81 -0.5 3,973,489 + PS417_18230 0.87 +0.3 3,973,490 - PS417_18230 0.87 -2.9 3,973,618 + +0.3 3,973,733 - -0.8 3,973,932 + PS417_18235 0.29 +0.7 3,974,016 - PS417_18235 0.43 -0.5 3,974,241 - PS417_18235 0.81 +0.0 3,974,457 + -0.0 3,974,477 + PS417_18240 0.12 +0.3 3,974,477 + PS417_18240 0.12 +0.1 3,974,507 - PS417_18240 0.17 -0.3 3,974,615 - PS417_18240 0.33 -0.6 3,974,623 + PS417_18240 0.34 +0.3 3,974,623 + PS417_18240 0.34 +0.5 3,974,624 - PS417_18240 0.35 +0.0 3,974,624 - PS417_18240 0.35 +0.5
Or see this region's nucleotide sequence