Strain Fitness in Pseudomonas simiae WCS417 around PS417_15020

Experiment: L-Isoleucine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_15010 and PS417_15015 are separated by 184 nucleotidesPS417_15015 and PS417_15020 are separated by 53 nucleotidesPS417_15020 and PS417_15025 are separated by 129 nucleotides PS417_15010: PS417_15010 - TetR family transcriptional regulator, at 3,282,082 to 3,282,675 _15010 PS417_15015: PS417_15015 - isochorismatase, at 3,282,860 to 3,283,384 _15015 PS417_15020: PS417_15020 - 2-hydroxyacid dehydrogenase, at 3,283,438 to 3,284,391 _15020 PS417_15025: PS417_15025 - aminoglycoside phosphotransferase, at 3,284,521 to 3,285,588 _15025 Position (kb) 3283 3284 3285Strain fitness (log2 ratio) -1 0 1at 3282.465 kb on + strand, within PS417_15010at 3282.465 kb on + strand, within PS417_15010at 3282.465 kb on + strand, within PS417_15010at 3282.466 kb on - strand, within PS417_15010at 3282.466 kb on - strand, within PS417_15010at 3282.466 kb on - strand, within PS417_15010at 3282.466 kb on - strand, within PS417_15010at 3282.466 kb on - strand, within PS417_15010at 3282.471 kb on + strand, within PS417_15010at 3282.499 kb on - strand, within PS417_15010at 3282.500 kb on + strand, within PS417_15010at 3282.500 kb on + strand, within PS417_15010at 3282.501 kb on - strand, within PS417_15010at 3282.768 kb on - strandat 3282.822 kb on - strandat 3282.900 kb on + strandat 3282.903 kb on - strandat 3283.402 kb on - strandat 3283.402 kb on - strandat 3283.480 kb on + strandat 3283.481 kb on - strandat 3283.609 kb on + strand, within PS417_15020at 3283.680 kb on - strand, within PS417_15020at 3283.680 kb on - strand, within PS417_15020at 3283.945 kb on + strand, within PS417_15020at 3283.978 kb on + strand, within PS417_15020at 3283.978 kb on + strand, within PS417_15020at 3284.136 kb on - strand, within PS417_15020at 3284.335 kb on + strandat 3284.335 kb on + strandat 3284.336 kb on - strandat 3284.336 kb on - strandat 3284.336 kb on - strandat 3284.430 kb on + strandat 3284.430 kb on + strandat 3284.430 kb on + strandat 3284.431 kb on - strandat 3284.431 kb on - strandat 3284.433 kb on + strandat 3284.433 kb on + strandat 3284.434 kb on - strandat 3284.472 kb on + strandat 3284.590 kb on + strandat 3284.668 kb on + strand, within PS417_15025at 3284.668 kb on + strand, within PS417_15025at 3284.668 kb on + strand, within PS417_15025at 3284.669 kb on - strand, within PS417_15025at 3284.669 kb on - strand, within PS417_15025at 3284.809 kb on + strand, within PS417_15025at 3284.810 kb on - strand, within PS417_15025at 3284.851 kb on + strandat 3285.104 kb on + strand, within PS417_15025at 3285.324 kb on + strand, within PS417_15025at 3285.324 kb on + strand, within PS417_15025at 3285.325 kb on - strand, within PS417_15025at 3285.382 kb on + strand, within PS417_15025

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (C)
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3,282,465 + PS417_15010 0.64 +0.0
3,282,465 + PS417_15010 0.64 -0.2
3,282,465 + PS417_15010 0.64 +0.3
3,282,466 - PS417_15010 0.65 -0.3
3,282,466 - PS417_15010 0.65 -0.0
3,282,466 - PS417_15010 0.65 +0.4
3,282,466 - PS417_15010 0.65 +0.3
3,282,466 - PS417_15010 0.65 -0.5
3,282,471 + PS417_15010 0.65 -0.1
3,282,499 - PS417_15010 0.70 -1.1
3,282,500 + PS417_15010 0.70 +0.6
3,282,500 + PS417_15010 0.70 -0.1
3,282,501 - PS417_15010 0.71 -0.1
3,282,768 - -0.8
3,282,822 - +1.6
3,282,900 + -0.4
3,282,903 - -0.4
3,283,402 - +0.0
3,283,402 - -0.1
3,283,480 + +0.7
3,283,481 - +0.3
3,283,609 + PS417_15020 0.18 +0.0
3,283,680 - PS417_15020 0.25 +0.0
3,283,680 - PS417_15020 0.25 -0.3
3,283,945 + PS417_15020 0.53 +0.6
3,283,978 + PS417_15020 0.57 -0.2
3,283,978 + PS417_15020 0.57 +0.0
3,284,136 - PS417_15020 0.73 -0.0
3,284,335 + +0.2
3,284,335 + +0.5
3,284,336 - -0.4
3,284,336 - -0.8
3,284,336 - +0.0
3,284,430 + +0.8
3,284,430 + -0.1
3,284,430 + -0.5
3,284,431 - -0.1
3,284,431 - -0.3
3,284,433 + -1.0
3,284,433 + +0.2
3,284,434 - +1.2
3,284,472 + -0.8
3,284,590 + +0.4
3,284,668 + PS417_15025 0.14 +0.3
3,284,668 + PS417_15025 0.14 +0.4
3,284,668 + PS417_15025 0.14 -0.6
3,284,669 - PS417_15025 0.14 -0.1
3,284,669 - PS417_15025 0.14 +1.3
3,284,809 + PS417_15025 0.27 -1.0
3,284,810 - PS417_15025 0.27 -0.7
3,284,851 + -1.0
3,285,104 + PS417_15025 0.55 +0.9
3,285,324 + PS417_15025 0.75 +0.4
3,285,324 + PS417_15025 0.75 +0.6
3,285,325 - PS417_15025 0.75 -1.2
3,285,382 + PS417_15025 0.81 -0.2

Or see this region's nucleotide sequence