Experiment: L-Valine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_23715 and PS417_23720 are separated by 48 nucleotides PS417_23720 and PS417_23725 are separated by 15 nucleotides PS417_23725 and PS417_23730 are separated by 131 nucleotides
PS417_23715: PS417_23715 - cupin, at 5,127,884 to 5,128,228
_23715
PS417_23720: PS417_23720 - 3-hydroxyisobutyrate dehydrogenase, at 5,128,277 to 5,129,164
_23720
PS417_23725: PS417_23725 - methylmalonate-semialdehyde dehydrogenase, at 5,129,180 to 5,130,697
_23725
PS417_23730: PS417_23730 - LysR family transcriptional regulator, at 5,130,829 to 5,131,776
_23730
Position (kb) 5129
5130
5131 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 5128.207 kb on + strand at 5128.208 kb on - strand at 5128.279 kb on - strand at 5128.300 kb on + strand at 5128.301 kb on - strand at 5128.304 kb on - strand at 5128.512 kb on + strand, within PS417_23720 at 5128.512 kb on + strand at 5128.513 kb on - strand, within PS417_23720 at 5129.541 kb on + strand, within PS417_23725 at 5130.273 kb on + strand, within PS417_23725 at 5130.273 kb on + strand, within PS417_23725 at 5130.274 kb on - strand, within PS417_23725 at 5130.277 kb on + strand, within PS417_23725 at 5130.716 kb on + strand at 5130.717 kb on - strand at 5130.717 kb on - strand at 5130.720 kb on + strand at 5130.721 kb on - strand at 5130.721 kb on - strand at 5130.782 kb on + strand at 5130.782 kb on + strand at 5130.782 kb on + strand at 5130.783 kb on - strand at 5130.783 kb on - strand at 5130.783 kb on - strand at 5130.783 kb on - strand at 5130.944 kb on - strand, within PS417_23730 at 5130.944 kb on - strand, within PS417_23730 at 5131.316 kb on - strand, within PS417_23730 at 5131.316 kb on - strand, within PS417_23730 at 5131.355 kb on - strand, within PS417_23730 at 5131.369 kb on + strand, within PS417_23730 at 5131.370 kb on - strand, within PS417_23730 at 5131.370 kb on - strand, within PS417_23730 at 5131.426 kb on - strand, within PS417_23730 at 5131.432 kb on + strand, within PS417_23730 at 5131.432 kb on + strand, within PS417_23730 at 5131.432 kb on + strand, within PS417_23730 at 5131.432 kb on + strand, within PS417_23730 at 5131.512 kb on + strand, within PS417_23730 at 5131.531 kb on + strand, within PS417_23730 at 5131.531 kb on + strand, within PS417_23730 at 5131.532 kb on - strand, within PS417_23730 at 5131.627 kb on - strand, within PS417_23730 at 5131.651 kb on + strand at 5131.651 kb on + strand, within PS417_23730 at 5131.651 kb on + strand, within PS417_23730 at 5131.652 kb on - strand, within PS417_23730 at 5131.686 kb on + strand at 5131.686 kb on + strand at 5131.687 kb on - strand at 5131.687 kb on - strand at 5131.687 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine (C) remove 5,128,207 + +1.9 5,128,208 - +1.1 5,128,279 - +0.3 5,128,300 + -3.6 5,128,301 - -1.3 5,128,304 - -5.0 5,128,512 + PS417_23720 0.26 -2.5 5,128,512 + +0.1 5,128,513 - PS417_23720 0.27 +0.7 5,129,541 + PS417_23725 0.24 -2.5 5,130,273 + PS417_23725 0.72 -4.8 5,130,273 + PS417_23725 0.72 -1.5 5,130,274 - PS417_23725 0.72 -2.3 5,130,277 + PS417_23725 0.72 +0.1 5,130,716 + -0.9 5,130,717 - +0.7 5,130,717 - -2.4 5,130,720 + -3.7 5,130,721 - -2.9 5,130,721 - -0.8 5,130,782 + -4.6 5,130,782 + -4.1 5,130,782 + -0.8 5,130,783 - -2.9 5,130,783 - -3.9 5,130,783 - -1.0 5,130,783 - -1.9 5,130,944 - PS417_23730 0.12 -2.1 5,130,944 - PS417_23730 0.12 -0.9 5,131,316 - PS417_23730 0.51 -2.7 5,131,316 - PS417_23730 0.51 +0.7 5,131,355 - PS417_23730 0.55 -3.1 5,131,369 + PS417_23730 0.57 -4.1 5,131,370 - PS417_23730 0.57 -1.6 5,131,370 - PS417_23730 0.57 -5.2 5,131,426 - PS417_23730 0.63 -3.7 5,131,432 + PS417_23730 0.64 -3.1 5,131,432 + PS417_23730 0.64 -2.7 5,131,432 + PS417_23730 0.64 -2.3 5,131,432 + PS417_23730 0.64 -5.6 5,131,512 + PS417_23730 0.72 -2.9 5,131,531 + PS417_23730 0.74 -3.2 5,131,531 + PS417_23730 0.74 -2.6 5,131,532 - PS417_23730 0.74 -3.3 5,131,627 - PS417_23730 0.84 -4.0 5,131,651 + -0.3 5,131,651 + PS417_23730 0.87 -3.9 5,131,651 + PS417_23730 0.87 -1.8 5,131,652 - PS417_23730 0.87 -2.6 5,131,686 + -3.9 5,131,686 + -1.1 5,131,687 - -3.9 5,131,687 - -3.3 5,131,687 - -3.2
Or see this region's nucleotide sequence