Experiment: L-Valine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_18520 and PS417_18525 are separated by 107 nucleotides PS417_18525 and PS417_18530 overlap by 4 nucleotides PS417_18530 and PS417_18535 are separated by 65 nucleotides
PS417_18520: PS417_18520 - tRNA-Val, at 4,022,191 to 4,022,266
_18520
PS417_18525: PS417_18525 - oxidoreductase, at 4,022,374 to 4,023,153
_18525
PS417_18530: PS417_18530 - O-succinylhomoserine sulfhydrylase, at 4,023,150 to 4,024,361
_18530
PS417_18535: PS417_18535 - amidophosphoribosyltransferase, at 4,024,427 to 4,025,932
_18535
Position (kb)
4023
4024
4025 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 4022.233 kb on - strand, within PS417_18520 at 4022.259 kb on - strand at 4022.259 kb on - strand at 4022.385 kb on - strand at 4022.908 kb on + strand, within PS417_18525 at 4023.300 kb on - strand, within PS417_18530 at 4023.303 kb on - strand, within PS417_18530 at 4023.937 kb on + strand, within PS417_18530 at 4023.937 kb on + strand, within PS417_18530 at 4023.937 kb on + strand, within PS417_18530 at 4024.152 kb on - strand, within PS417_18530 at 4024.152 kb on - strand, within PS417_18530 at 4024.162 kb on + strand, within PS417_18530 at 4024.162 kb on + strand, within PS417_18530 at 4024.226 kb on - strand, within PS417_18530 at 4024.311 kb on + strand at 4024.311 kb on + strand at 4024.312 kb on - strand at 4024.312 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Valine (C) remove 4,022,233 - PS417_18520 0.55 +1.5 4,022,259 - -1.0 4,022,259 - +0.4 4,022,385 - +1.7 4,022,908 + PS417_18525 0.68 -1.7 4,023,300 - PS417_18530 0.12 -3.1 4,023,303 - PS417_18530 0.13 -1.7 4,023,937 + PS417_18530 0.65 -4.0 4,023,937 + PS417_18530 0.65 -2.1 4,023,937 + PS417_18530 0.65 -2.0 4,024,152 - PS417_18530 0.83 -5.1 4,024,152 - PS417_18530 0.83 -6.0 4,024,162 + PS417_18530 0.83 -4.5 4,024,162 + PS417_18530 0.83 -5.1 4,024,226 - PS417_18530 0.89 -1.4 4,024,311 + -3.1 4,024,311 + -4.2 4,024,312 - -3.5 4,024,312 - -5.9
Or see this region's nucleotide sequence