Strain Fitness in Pseudomonas simiae WCS417 around PS417_13850

Experiment: L-Valine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_13840 and PS417_13845 are separated by 61 nucleotidesPS417_13845 and PS417_13850 are separated by 11 nucleotidesPS417_13850 and PS417_13855 are separated by 9 nucleotides PS417_13840: PS417_13840 - isobutyryl-CoA dehydrogenase (EC 1.3.8.5) (from data), at 3,040,452 to 3,041,603 _13840 PS417_13845: PS417_13845 - enoyl-CoA hydratase, at 3,041,665 to 3,042,438 _13845 PS417_13850: PS417_13850 - 2-methylbutanoyl-CoA dehydrogenase (EC 1.3.8.5) (from data), at 3,042,450 to 3,043,577 _13850 PS417_13855: PS417_13855 - acetyl-CoA acetyltransferase, at 3,043,587 to 3,044,771 _13855 Position (kb) 3042 3043 3044Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 3041.481 kb on + strand, within PS417_13840at 3041.482 kb on - strand, within PS417_13840at 3041.482 kb on - strand, within PS417_13840at 3041.614 kb on + strandat 3043.006 kb on + strandat 3043.137 kb on + strand, within PS417_13850at 3043.138 kb on - strand, within PS417_13850at 3043.141 kb on + strand, within PS417_13850at 3043.141 kb on + strand, within PS417_13850at 3043.142 kb on - strand, within PS417_13850at 3043.142 kb on - strand, within PS417_13850at 3043.178 kb on + strand, within PS417_13850at 3043.378 kb on + strand, within PS417_13850at 3043.591 kb on + strandat 3043.592 kb on - strandat 3044.401 kb on + strand, within PS417_13855

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Valine (C)
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3,041,481 + PS417_13840 0.89 -2.4
3,041,482 - PS417_13840 0.89 -3.1
3,041,482 - PS417_13840 0.89 -2.9
3,041,614 + +0.6
3,043,006 + +0.1
3,043,137 + PS417_13850 0.61 -1.4
3,043,138 - PS417_13850 0.61 -0.5
3,043,141 + PS417_13850 0.61 -4.0
3,043,141 + PS417_13850 0.61 -1.4
3,043,142 - PS417_13850 0.61 -1.5
3,043,142 - PS417_13850 0.61 +2.4
3,043,178 + PS417_13850 0.65 -2.1
3,043,378 + PS417_13850 0.82 +0.4
3,043,591 + -1.7
3,043,592 - +0.9
3,044,401 + PS417_13855 0.69 -0.9

Or see this region's nucleotide sequence