Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5541 and Atu5542 are separated by 62 nucleotides Atu5542 and Atu5543 are separated by 224 nucleotides Atu5543 and Atu5544 are separated by 40 nucleotides Atu5544 and Atu5545 are separated by 165 nucleotides Atu5545 and Atu8053 are separated by 29 nucleotides
Atu5541: Atu5541 - hypothetical protein, at 535,269 to 537,218
Atu5541
Atu5542: Atu5542 - hypothetical protein, at 537,281 to 537,658
Atu5542
Atu5543: Atu5543 - AraC family transcriptional regulator, at 537,883 to 538,530
Atu5543
Atu5544: Atu5544 - hypothetical protein, at 538,571 to 538,945
Atu5544
Atu5545: Atu5545 - hypothetical protein, at 539,111 to 539,338
Atu5545
Atu8053: Atu8053 - hypothetical protein, at 539,368 to 539,553
Atu8053
Position (kb)
537
538
539 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 536.948 kb on + strand, within Atu5541 at 536.948 kb on + strand, within Atu5541 at 536.980 kb on - strand, within Atu5541 at 536.989 kb on - strand, within Atu5541 at 537.076 kb on - strand at 537.161 kb on + strand at 537.161 kb on + strand at 537.188 kb on - strand at 537.280 kb on + strand at 537.298 kb on + strand at 537.306 kb on - strand at 537.308 kb on + strand at 537.308 kb on + strand at 537.316 kb on - strand at 537.316 kb on - strand at 537.318 kb on + strand at 537.318 kb on + strand at 537.320 kb on + strand, within Atu5542 at 537.320 kb on + strand, within Atu5542 at 537.325 kb on + strand, within Atu5542 at 537.328 kb on - strand, within Atu5542 at 537.353 kb on + strand, within Atu5542 at 537.353 kb on + strand, within Atu5542 at 537.361 kb on - strand, within Atu5542 at 537.378 kb on + strand, within Atu5542 at 537.396 kb on + strand, within Atu5542 at 537.401 kb on + strand, within Atu5542 at 537.401 kb on + strand, within Atu5542 at 537.454 kb on + strand, within Atu5542 at 537.464 kb on + strand, within Atu5542 at 537.468 kb on - strand, within Atu5542 at 537.472 kb on - strand, within Atu5542 at 537.474 kb on + strand, within Atu5542 at 537.532 kb on - strand, within Atu5542 at 537.579 kb on - strand, within Atu5542 at 537.607 kb on + strand, within Atu5542 at 537.623 kb on + strand at 537.638 kb on + strand at 537.802 kb on + strand at 537.802 kb on + strand at 537.826 kb on - strand at 537.856 kb on + strand at 537.871 kb on + strand at 537.879 kb on + strand at 537.896 kb on - strand at 537.913 kb on + strand at 537.973 kb on + strand, within Atu5543 at 538.042 kb on - strand, within Atu5543 at 538.044 kb on + strand, within Atu5543 at 538.071 kb on + strand, within Atu5543 at 538.146 kb on - strand, within Atu5543 at 538.151 kb on - strand, within Atu5543 at 538.173 kb on - strand, within Atu5543 at 538.198 kb on - strand, within Atu5543 at 538.283 kb on + strand, within Atu5543 at 538.284 kb on + strand, within Atu5543 at 538.342 kb on + strand, within Atu5543 at 538.390 kb on + strand, within Atu5543 at 538.390 kb on + strand, within Atu5543 at 538.397 kb on + strand, within Atu5543 at 538.397 kb on + strand, within Atu5543 at 538.491 kb on - strand at 538.553 kb on + strand at 538.582 kb on + strand at 538.619 kb on - strand, within Atu5544 at 538.619 kb on - strand, within Atu5544 at 538.729 kb on + strand, within Atu5544 at 538.788 kb on + strand, within Atu5544 at 538.788 kb on + strand, within Atu5544 at 538.788 kb on + strand, within Atu5544 at 538.788 kb on + strand, within Atu5544 at 538.788 kb on + strand, within Atu5544 at 538.796 kb on - strand, within Atu5544 at 538.796 kb on - strand, within Atu5544 at 538.809 kb on + strand, within Atu5544 at 538.843 kb on + strand, within Atu5544 at 538.851 kb on - strand, within Atu5544 at 539.015 kb on + strand at 539.029 kb on - strand at 539.078 kb on - strand at 539.102 kb on + strand at 539.176 kb on + strand, within Atu5545 at 539.181 kb on + strand, within Atu5545 at 539.265 kb on - strand, within Atu5545 at 539.268 kb on + strand, within Atu5545 at 539.275 kb on - strand, within Atu5545 at 539.379 kb on + strand at 539.462 kb on + strand, within Atu8053 at 539.462 kb on + strand, within Atu8053 at 539.463 kb on + strand, within Atu8053 at 539.505 kb on - strand, within Atu8053
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 536,948 + Atu5541 0.86 +0.7 536,948 + Atu5541 0.86 -1.4 536,980 - Atu5541 0.88 +0.8 536,989 - Atu5541 0.88 +0.1 537,076 - +0.4 537,161 + -0.5 537,161 + +0.4 537,188 - +1.1 537,280 + -0.7 537,298 + -0.4 537,306 - +0.1 537,308 + +2.5 537,308 + +0.2 537,316 - -0.9 537,316 - +0.0 537,318 + +0.6 537,318 + +2.2 537,320 + Atu5542 0.10 -0.0 537,320 + Atu5542 0.10 -0.3 537,325 + Atu5542 0.12 +0.0 537,328 - Atu5542 0.12 +0.3 537,353 + Atu5542 0.19 -0.4 537,353 + Atu5542 0.19 +1.5 537,361 - Atu5542 0.21 +0.1 537,378 + Atu5542 0.26 -0.2 537,396 + Atu5542 0.30 +1.1 537,401 + Atu5542 0.32 +1.2 537,401 + Atu5542 0.32 +2.6 537,454 + Atu5542 0.46 +0.3 537,464 + Atu5542 0.48 +0.8 537,468 - Atu5542 0.49 +1.3 537,472 - Atu5542 0.51 +0.8 537,474 + Atu5542 0.51 +0.3 537,532 - Atu5542 0.66 +0.7 537,579 - Atu5542 0.79 +0.3 537,607 + Atu5542 0.86 -0.1 537,623 + +2.0 537,638 + +1.5 537,802 + +0.1 537,802 + -0.2 537,826 - +0.2 537,856 + -0.2 537,871 + +0.2 537,879 + +2.2 537,896 - -2.2 537,913 + +0.1 537,973 + Atu5543 0.14 +0.7 538,042 - Atu5543 0.25 -1.0 538,044 + Atu5543 0.25 -0.7 538,071 + Atu5543 0.29 -0.1 538,146 - Atu5543 0.41 -1.4 538,151 - Atu5543 0.41 -0.3 538,173 - Atu5543 0.45 +0.5 538,198 - Atu5543 0.49 +0.1 538,283 + Atu5543 0.62 -0.0 538,284 + Atu5543 0.62 -1.5 538,342 + Atu5543 0.71 +0.2 538,390 + Atu5543 0.78 +0.6 538,390 + Atu5543 0.78 -0.1 538,397 + Atu5543 0.79 +0.5 538,397 + Atu5543 0.79 -0.6 538,491 - -0.7 538,553 + -0.3 538,582 + +0.8 538,619 - Atu5544 0.13 +1.5 538,619 - Atu5544 0.13 +0.4 538,729 + Atu5544 0.42 +0.8 538,788 + Atu5544 0.58 -0.1 538,788 + Atu5544 0.58 +0.7 538,788 + Atu5544 0.58 +0.2 538,788 + Atu5544 0.58 +0.5 538,788 + Atu5544 0.58 +0.2 538,796 - Atu5544 0.60 +0.6 538,796 - Atu5544 0.60 -0.5 538,809 + Atu5544 0.63 -0.8 538,843 + Atu5544 0.73 +0.5 538,851 - Atu5544 0.75 +0.1 539,015 + +0.0 539,029 - +0.6 539,078 - +0.6 539,102 + +0.5 539,176 + Atu5545 0.29 +0.1 539,181 + Atu5545 0.31 +0.3 539,265 - Atu5545 0.68 -0.5 539,268 + Atu5545 0.69 -0.0 539,275 - Atu5545 0.72 -1.5 539,379 + -0.1 539,462 + Atu8053 0.51 +0.4 539,462 + Atu8053 0.51 -0.0 539,463 + Atu8053 0.51 +1.8 539,505 - Atu8053 0.74 -0.2
Or see this region's nucleotide sequence