Experiment: Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ygiT and b3022 are separated by 1 nucleotides b3022 and ygiV are separated by 204 nucleotides ygiV and ygiW are separated by 52 nucleotides ygiW and qseB are separated by 151 nucleotides
b3021: ygiT - predicted DNA-binding transcriptional regulator (NCBI), at 3,165,873 to 3,166,268
ygiT
b3022: b3022 - predicted cyanide hydratase (NCBI), at 3,166,270 to 3,166,566
b3022
b3023: ygiV - predicted transcriptional regulator (NCBI), at 3,166,771 to 3,167,253
ygiV
b3024: ygiW - hypothetical protein (NCBI), at 3,167,306 to 3,167,698
ygiW
b3025: qseB - DNA-binding response regulator in two-component regulatory system with QseC (NCBI), at 3,167,850 to 3,168,509
qseB
Position (kb)
3166
3167
3168 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3165.775 kb on + strand at 3165.784 kb on - strand at 3165.798 kb on + strand at 3165.798 kb on + strand at 3165.822 kb on + strand at 3165.822 kb on + strand at 3165.895 kb on + strand at 3165.903 kb on + strand at 3165.914 kb on - strand, within ygiT at 3165.946 kb on - strand, within ygiT at 3165.950 kb on - strand, within ygiT at 3165.950 kb on - strand, within ygiT at 3165.985 kb on - strand, within ygiT at 3166.018 kb on - strand, within ygiT at 3166.093 kb on + strand, within ygiT at 3166.285 kb on - strand at 3166.285 kb on - strand at 3166.286 kb on - strand at 3166.290 kb on - strand at 3166.290 kb on - strand at 3166.299 kb on - strand at 3166.308 kb on - strand, within b3022 at 3166.308 kb on - strand, within b3022 at 3166.325 kb on - strand, within b3022 at 3166.330 kb on - strand, within b3022 at 3166.330 kb on - strand, within b3022 at 3166.335 kb on - strand, within b3022 at 3166.337 kb on + strand, within b3022 at 3166.345 kb on - strand, within b3022 at 3166.345 kb on - strand, within b3022 at 3166.346 kb on - strand, within b3022 at 3166.357 kb on - strand, within b3022 at 3166.370 kb on + strand, within b3022 at 3166.376 kb on - strand, within b3022 at 3166.377 kb on + strand, within b3022 at 3166.382 kb on + strand, within b3022 at 3166.382 kb on + strand, within b3022 at 3166.388 kb on - strand, within b3022 at 3166.388 kb on - strand, within b3022 at 3166.390 kb on - strand, within b3022 at 3166.420 kb on - strand, within b3022 at 3166.441 kb on - strand, within b3022 at 3166.480 kb on - strand, within b3022 at 3166.480 kb on - strand, within b3022 at 3166.523 kb on + strand, within b3022 at 3166.539 kb on + strand at 3166.551 kb on - strand at 3166.551 kb on - strand at 3166.551 kb on - strand at 3166.576 kb on - strand at 3166.576 kb on - strand at 3166.696 kb on - strand at 3166.703 kb on + strand at 3166.703 kb on + strand at 3166.771 kb on - strand at 3166.795 kb on - strand at 3166.795 kb on - strand at 3166.843 kb on + strand, within ygiV at 3166.909 kb on - strand, within ygiV at 3166.956 kb on - strand, within ygiV at 3166.956 kb on - strand, within ygiV at 3166.989 kb on + strand, within ygiV at 3166.989 kb on + strand, within ygiV at 3167.060 kb on + strand, within ygiV at 3167.154 kb on + strand, within ygiV at 3167.158 kb on + strand, within ygiV at 3167.175 kb on + strand, within ygiV at 3167.175 kb on + strand, within ygiV at 3167.175 kb on + strand, within ygiV at 3167.192 kb on + strand, within ygiV at 3167.227 kb on + strand at 3167.422 kb on - strand, within ygiW at 3167.470 kb on + strand, within ygiW at 3167.470 kb on + strand, within ygiW at 3167.470 kb on + strand, within ygiW at 3167.478 kb on - strand, within ygiW at 3167.478 kb on - strand, within ygiW at 3167.500 kb on - strand, within ygiW at 3167.500 kb on - strand, within ygiW at 3167.512 kb on + strand, within ygiW at 3167.652 kb on + strand, within ygiW at 3167.718 kb on - strand at 3167.793 kb on - strand at 3167.793 kb on - strand at 3167.798 kb on + strand at 3167.800 kb on + strand at 3167.800 kb on + strand at 3167.800 kb on + strand at 3167.979 kb on + strand, within qseB at 3167.987 kb on - strand, within qseB at 3167.987 kb on - strand, within qseB at 3168.014 kb on - strand, within qseB at 3168.014 kb on - strand, within qseB at 3168.045 kb on + strand, within qseB at 3168.096 kb on + strand, within qseB at 3168.108 kb on + strand, within qseB at 3168.112 kb on + strand, within qseB at 3168.179 kb on - strand, within qseB at 3168.192 kb on + strand, within qseB at 3168.235 kb on + strand, within qseB
Per-strain Table
Position Strand Gene LocusTag Fraction Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB remove 3,165,775 + -0.7 3,165,784 - +1.3 3,165,798 + +0.1 3,165,798 + +0.3 3,165,822 + +0.1 3,165,822 + +0.9 3,165,895 + -0.7 3,165,903 + +1.8 3,165,914 - ygiT b3021 0.10 -0.5 3,165,946 - ygiT b3021 0.18 -1.0 3,165,950 - ygiT b3021 0.19 -0.2 3,165,950 - ygiT b3021 0.19 +1.0 3,165,985 - ygiT b3021 0.28 -0.6 3,166,018 - ygiT b3021 0.37 -1.3 3,166,093 + ygiT b3021 0.56 +1.8 3,166,285 - -0.3 3,166,285 - +0.5 3,166,286 - -0.1 3,166,290 - -0.6 3,166,290 - -0.0 3,166,299 - -0.0 3,166,308 - b3022 b3022 0.13 -0.1 3,166,308 - b3022 b3022 0.13 -0.4 3,166,325 - b3022 b3022 0.19 +0.2 3,166,330 - b3022 b3022 0.20 +0.5 3,166,330 - b3022 b3022 0.20 +0.3 3,166,335 - b3022 b3022 0.22 +0.3 3,166,337 + b3022 b3022 0.23 +1.8 3,166,345 - b3022 b3022 0.25 -0.0 3,166,345 - b3022 b3022 0.25 +0.3 3,166,346 - b3022 b3022 0.26 +0.1 3,166,357 - b3022 b3022 0.29 -0.2 3,166,370 + b3022 b3022 0.34 -1.0 3,166,376 - b3022 b3022 0.36 -0.3 3,166,377 + b3022 b3022 0.36 +1.5 3,166,382 + b3022 b3022 0.38 +1.8 3,166,382 + b3022 b3022 0.38 -1.6 3,166,388 - b3022 b3022 0.40 -0.3 3,166,388 - b3022 b3022 0.40 +0.2 3,166,390 - b3022 b3022 0.40 -0.2 3,166,420 - b3022 b3022 0.51 -0.1 3,166,441 - b3022 b3022 0.58 +0.7 3,166,480 - b3022 b3022 0.71 +0.4 3,166,480 - b3022 b3022 0.71 +0.6 3,166,523 + b3022 b3022 0.85 -0.4 3,166,539 + +0.1 3,166,551 - +0.4 3,166,551 - +0.0 3,166,551 - +0.1 3,166,576 - -0.2 3,166,576 - +0.7 3,166,696 - -0.4 3,166,703 + -0.9 3,166,703 + +0.5 3,166,771 - +0.1 3,166,795 - +0.5 3,166,795 - +0.2 3,166,843 + ygiV b3023 0.15 -0.5 3,166,909 - ygiV b3023 0.29 -0.4 3,166,956 - ygiV b3023 0.38 +0.1 3,166,956 - ygiV b3023 0.38 -0.2 3,166,989 + ygiV b3023 0.45 -0.3 3,166,989 + ygiV b3023 0.45 -0.1 3,167,060 + ygiV b3023 0.60 +0.1 3,167,154 + ygiV b3023 0.79 -1.9 3,167,158 + ygiV b3023 0.80 -0.1 3,167,175 + ygiV b3023 0.84 -1.4 3,167,175 + ygiV b3023 0.84 +0.1 3,167,175 + ygiV b3023 0.84 -0.2 3,167,192 + ygiV b3023 0.87 -0.2 3,167,227 + +0.0 3,167,422 - ygiW b3024 0.30 -0.7 3,167,470 + ygiW b3024 0.42 -0.2 3,167,470 + ygiW b3024 0.42 +0.4 3,167,470 + ygiW b3024 0.42 -1.2 3,167,478 - ygiW b3024 0.44 +0.1 3,167,478 - ygiW b3024 0.44 +0.2 3,167,500 - ygiW b3024 0.49 +0.5 3,167,500 - ygiW b3024 0.49 +1.0 3,167,512 + ygiW b3024 0.52 +0.6 3,167,652 + ygiW b3024 0.88 -0.2 3,167,718 - -0.5 3,167,793 - +0.0 3,167,793 - +0.5 3,167,798 + -0.3 3,167,800 + -0.2 3,167,800 + +0.1 3,167,800 + -1.5 3,167,979 + qseB b3025 0.20 -0.7 3,167,987 - qseB b3025 0.21 +0.6 3,167,987 - qseB b3025 0.21 -1.0 3,168,014 - qseB b3025 0.25 -0.2 3,168,014 - qseB b3025 0.25 -0.4 3,168,045 + qseB b3025 0.30 -0.3 3,168,096 + qseB b3025 0.37 -0.4 3,168,108 + qseB b3025 0.39 -0.2 3,168,112 + qseB b3025 0.40 +0.1 3,168,179 - qseB b3025 0.50 +0.5 3,168,192 + qseB b3025 0.52 -0.1 3,168,235 + qseB b3025 0.58 -0.2
Or see this region's nucleotide sequence