Experiment: Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ygeD and aas overlap by 8 nucleotides aas and rygA are separated by 87 nucleotides rygA and omrB are separated by 114 nucleotides omrB and galR are separated by 213 nucleotides
b2835: ygeD - predicted inner membrane protein (NCBI), at 2,970,691 to 2,971,884
ygeD
b2836: aas - 2-acyl-glycerophospho-ethanolamine acyltransferase (NCBI), at 2,971,877 to 2,974,036
aas
b4444: rygA - unknown RNA (NCBI), at 2,974,124 to 2,974,211
rygA
b4445: omrB - sRNA down regulates OM proteins; positively regulated by OmpR/EnvZ; binds Hfq (RefSeq), at 2,974,326 to 2,974,407
omrB
b2837: galR - DNA-binding transcriptional repressor (NCBI), at 2,974,621 to 2,975,652
galR
Position (kb)
2971
2972
2973
2974
2975 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2970.900 kb on - strand, within ygeD at 2970.970 kb on - strand, within ygeD at 2971.028 kb on + strand, within ygeD at 2971.028 kb on + strand, within ygeD at 2971.031 kb on - strand, within ygeD at 2971.031 kb on - strand, within ygeD at 2971.033 kb on - strand, within ygeD at 2971.068 kb on - strand, within ygeD at 2971.154 kb on - strand, within ygeD at 2971.180 kb on + strand, within ygeD at 2971.303 kb on - strand, within ygeD at 2971.305 kb on + strand, within ygeD at 2971.360 kb on + strand, within ygeD at 2971.360 kb on + strand, within ygeD at 2971.438 kb on - strand, within ygeD at 2971.489 kb on + strand, within ygeD at 2971.489 kb on + strand, within ygeD at 2971.489 kb on + strand, within ygeD at 2971.571 kb on - strand, within ygeD at 2971.581 kb on + strand, within ygeD at 2971.617 kb on + strand, within ygeD at 2971.625 kb on - strand, within ygeD at 2971.625 kb on - strand, within ygeD at 2971.634 kb on + strand, within ygeD at 2971.656 kb on - strand, within ygeD at 2971.656 kb on - strand, within ygeD at 2971.737 kb on - strand, within ygeD at 2971.737 kb on - strand, within ygeD at 2971.753 kb on - strand, within ygeD at 2971.776 kb on - strand at 2971.857 kb on + strand at 2971.864 kb on + strand at 2971.869 kb on + strand at 2971.869 kb on + strand at 2971.970 kb on + strand at 2972.106 kb on - strand, within aas at 2972.202 kb on - strand, within aas at 2972.210 kb on + strand, within aas at 2972.215 kb on + strand, within aas at 2972.215 kb on + strand, within aas at 2972.239 kb on + strand, within aas at 2972.261 kb on - strand, within aas at 2972.261 kb on - strand, within aas at 2972.467 kb on + strand, within aas at 2972.498 kb on - strand, within aas at 2972.589 kb on - strand, within aas at 2972.629 kb on + strand, within aas at 2972.660 kb on - strand, within aas at 2972.660 kb on - strand, within aas at 2972.667 kb on + strand, within aas at 2972.668 kb on - strand, within aas at 2972.750 kb on - strand, within aas at 2972.750 kb on - strand, within aas at 2972.752 kb on - strand, within aas at 2972.752 kb on - strand, within aas at 2972.776 kb on + strand, within aas at 2972.789 kb on + strand, within aas at 2972.887 kb on - strand, within aas at 2972.905 kb on + strand, within aas at 2972.952 kb on + strand, within aas at 2973.147 kb on + strand, within aas at 2973.200 kb on - strand, within aas at 2973.253 kb on + strand, within aas at 2973.261 kb on - strand, within aas at 2973.261 kb on - strand, within aas at 2973.351 kb on + strand, within aas at 2973.351 kb on + strand, within aas at 2973.354 kb on - strand, within aas at 2973.354 kb on - strand, within aas at 2973.526 kb on - strand, within aas at 2973.540 kb on - strand, within aas at 2973.699 kb on - strand, within aas at 2973.741 kb on + strand, within aas at 2973.754 kb on + strand, within aas at 2973.831 kb on - strand at 2973.831 kb on - strand at 2973.842 kb on - strand at 2973.894 kb on - strand at 2973.916 kb on + strand at 2973.918 kb on + strand at 2973.974 kb on - strand at 2973.995 kb on + strand at 2974.053 kb on - strand at 2974.055 kb on + strand at 2974.055 kb on + strand at 2974.090 kb on + strand at 2974.090 kb on + strand at 2974.097 kb on - strand at 2974.148 kb on + strand, within rygA at 2974.161 kb on - strand, within rygA at 2974.163 kb on - strand, within rygA at 2974.163 kb on - strand, within rygA at 2974.168 kb on - strand, within rygA at 2974.174 kb on - strand, within rygA at 2974.183 kb on - strand, within rygA at 2974.340 kb on + strand, within omrB at 2974.353 kb on - strand, within omrB at 2974.363 kb on - strand, within omrB at 2974.381 kb on - strand, within omrB at 2974.429 kb on - strand at 2974.581 kb on - strand at 2974.635 kb on + strand at 2974.635 kb on + strand at 2974.636 kb on - strand at 2974.636 kb on - strand at 2974.684 kb on + strand at 2974.684 kb on + strand at 2974.723 kb on + strand at 2974.733 kb on + strand, within galR at 2974.733 kb on + strand, within galR at 2974.741 kb on - strand, within galR at 2974.741 kb on - strand, within galR at 2974.751 kb on + strand, within galR at 2974.793 kb on + strand, within galR at 2974.793 kb on + strand, within galR
Per-strain Table
Position Strand Gene LocusTag Fraction Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB remove 2,970,900 - ygeD b2835 0.18 -1.4 2,970,970 - ygeD b2835 0.23 -0.9 2,971,028 + ygeD b2835 0.28 -1.4 2,971,028 + ygeD b2835 0.28 -0.7 2,971,031 - ygeD b2835 0.28 -0.3 2,971,031 - ygeD b2835 0.28 -0.1 2,971,033 - ygeD b2835 0.29 -1.1 2,971,068 - ygeD b2835 0.32 -0.9 2,971,154 - ygeD b2835 0.39 -1.1 2,971,180 + ygeD b2835 0.41 -1.6 2,971,303 - ygeD b2835 0.51 -2.7 2,971,305 + ygeD b2835 0.51 -1.2 2,971,360 + ygeD b2835 0.56 -0.9 2,971,360 + ygeD b2835 0.56 -1.8 2,971,438 - ygeD b2835 0.63 -1.4 2,971,489 + ygeD b2835 0.67 -1.7 2,971,489 + ygeD b2835 0.67 +0.5 2,971,489 + ygeD b2835 0.67 -1.0 2,971,571 - ygeD b2835 0.74 -1.4 2,971,581 + ygeD b2835 0.75 -2.4 2,971,617 + ygeD b2835 0.78 -0.9 2,971,625 - ygeD b2835 0.78 -2.6 2,971,625 - ygeD b2835 0.78 -1.4 2,971,634 + ygeD b2835 0.79 -1.9 2,971,656 - ygeD b2835 0.81 -2.4 2,971,656 - ygeD b2835 0.81 -2.3 2,971,737 - ygeD b2835 0.88 -1.4 2,971,737 - ygeD b2835 0.88 -1.2 2,971,753 - ygeD b2835 0.89 -1.3 2,971,776 - -1.1 2,971,857 + -1.5 2,971,864 + -1.0 2,971,869 + -1.0 2,971,869 + -1.0 2,971,970 + -1.2 2,972,106 - aas b2836 0.11 -0.6 2,972,202 - aas b2836 0.15 -1.8 2,972,210 + aas b2836 0.15 -2.3 2,972,215 + aas b2836 0.16 -3.5 2,972,215 + aas b2836 0.16 -1.3 2,972,239 + aas b2836 0.17 -1.6 2,972,261 - aas b2836 0.18 -0.6 2,972,261 - aas b2836 0.18 -0.9 2,972,467 + aas b2836 0.27 -2.2 2,972,498 - aas b2836 0.29 -2.0 2,972,589 - aas b2836 0.33 -1.8 2,972,629 + aas b2836 0.35 -1.8 2,972,660 - aas b2836 0.36 -1.2 2,972,660 - aas b2836 0.36 -0.8 2,972,667 + aas b2836 0.37 -2.5 2,972,668 - aas b2836 0.37 -1.8 2,972,750 - aas b2836 0.40 -0.6 2,972,750 - aas b2836 0.40 -0.1 2,972,752 - aas b2836 0.41 -0.7 2,972,752 - aas b2836 0.41 -0.2 2,972,776 + aas b2836 0.42 -1.6 2,972,789 + aas b2836 0.42 -1.4 2,972,887 - aas b2836 0.47 -0.2 2,972,905 + aas b2836 0.48 +0.0 2,972,952 + aas b2836 0.50 -1.9 2,973,147 + aas b2836 0.59 -1.5 2,973,200 - aas b2836 0.61 -2.4 2,973,253 + aas b2836 0.64 -1.4 2,973,261 - aas b2836 0.64 -0.6 2,973,261 - aas b2836 0.64 -0.6 2,973,351 + aas b2836 0.68 -1.2 2,973,351 + aas b2836 0.68 -0.8 2,973,354 - aas b2836 0.68 -3.4 2,973,354 - aas b2836 0.68 -1.4 2,973,526 - aas b2836 0.76 -3.1 2,973,540 - aas b2836 0.77 -2.6 2,973,699 - aas b2836 0.84 -1.1 2,973,741 + aas b2836 0.86 -1.6 2,973,754 + aas b2836 0.87 +0.3 2,973,831 - -1.3 2,973,831 - -1.8 2,973,842 - -1.7 2,973,894 - -1.6 2,973,916 + -0.9 2,973,918 + -1.4 2,973,974 - -0.3 2,973,995 + -1.2 2,974,053 - -0.3 2,974,055 + -0.4 2,974,055 + -1.9 2,974,090 + -0.9 2,974,090 + -2.6 2,974,097 - +0.5 2,974,148 + rygA b4444 0.27 +0.1 2,974,161 - rygA b4444 0.42 +0.7 2,974,163 - rygA b4444 0.44 -0.3 2,974,163 - rygA b4444 0.44 +0.1 2,974,168 - rygA b4444 0.50 +0.1 2,974,174 - rygA b4444 0.57 +0.5 2,974,183 - rygA b4444 0.67 -1.3 2,974,340 + omrB b4445 0.17 +0.3 2,974,353 - omrB b4445 0.33 -0.6 2,974,363 - omrB b4445 0.45 +0.1 2,974,381 - omrB b4445 0.67 +0.0 2,974,429 - -0.0 2,974,581 - -0.7 2,974,635 + +0.1 2,974,635 + +0.4 2,974,636 - -0.3 2,974,636 - +1.3 2,974,684 + -1.1 2,974,684 + +0.2 2,974,723 + +0.1 2,974,733 + galR b2837 0.11 -2.3 2,974,733 + galR b2837 0.11 -3.8 2,974,741 - galR b2837 0.12 +0.1 2,974,741 - galR b2837 0.12 -1.9 2,974,751 + galR b2837 0.13 +0.1 2,974,793 + galR b2837 0.17 -0.1 2,974,793 + galR b2837 0.17 -1.2
Or see this region's nucleotide sequence