Experiment: Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ytfN and ytfP are separated by 2 nucleotides ytfP and chpS are separated by 211 nucleotides chpS and chpB overlap by 7 nucleotides chpB and ppa are separated by 79 nucleotides ppa and ytfQ are separated by 309 nucleotides
b4221: ytfN - hypothetical protein (NCBI), at 4,442,135 to 4,445,914
ytfN
b4222: ytfP - hypothetical protein (NCBI), at 4,445,917 to 4,446,258
ytfP
b4224: chpS - suppressor of inhibitory function of ChpB, PemI-like, autoregulated (VIMSS), at 4,446,470 to 4,446,721
chpS
b4225: chpB - toxin of the ChpB-ChpS toxin-antitoxin system (NCBI), at 4,446,715 to 4,447,065
chpB
b4226: ppa - inorganic pyrophosphatase (NCBI), at 4,447,145 to 4,447,675
ppa
b4227: ytfQ - predicted sugar transporter subunit: periplasmic-binding component of ABC superfamily (NCBI), at 4,447,985 to 4,448,941
ytfQ
Position (kb)
4446
4447
4448 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 4445.736 kb on + strand at 4445.737 kb on + strand at 4445.737 kb on + strand at 4445.770 kb on - strand at 4445.794 kb on + strand at 4445.806 kb on + strand at 4445.806 kb on + strand at 4445.806 kb on + strand at 4445.836 kb on + strand at 4445.836 kb on + strand at 4445.859 kb on - strand at 4445.866 kb on + strand at 4445.866 kb on + strand at 4445.962 kb on + strand, within ytfP at 4445.962 kb on + strand, within ytfP at 4446.046 kb on - strand, within ytfP at 4446.131 kb on + strand, within ytfP at 4446.166 kb on + strand, within ytfP at 4446.166 kb on + strand, within ytfP at 4446.166 kb on + strand, within ytfP at 4446.176 kb on + strand, within ytfP at 4446.176 kb on - strand, within ytfP at 4446.176 kb on - strand, within ytfP at 4446.176 kb on - strand, within ytfP at 4446.184 kb on - strand, within ytfP at 4446.184 kb on - strand, within ytfP at 4446.240 kb on + strand at 4446.259 kb on + strand at 4446.260 kb on + strand at 4446.272 kb on - strand at 4446.273 kb on - strand at 4446.273 kb on - strand at 4446.375 kb on - strand at 4446.454 kb on + strand at 4446.454 kb on + strand at 4446.496 kb on + strand, within chpS at 4446.501 kb on + strand, within chpS at 4446.501 kb on + strand, within chpS at 4446.509 kb on - strand, within chpS at 4446.509 kb on - strand, within chpS at 4446.514 kb on - strand, within chpS at 4446.559 kb on + strand, within chpS at 4446.569 kb on - strand, within chpS at 4446.569 kb on - strand, within chpS at 4446.646 kb on - strand, within chpS at 4446.652 kb on - strand, within chpS at 4446.746 kb on + strand at 4446.777 kb on + strand, within chpB at 4446.787 kb on + strand, within chpB at 4446.787 kb on + strand, within chpB at 4446.787 kb on - strand, within chpB at 4446.787 kb on - strand, within chpB at 4446.794 kb on + strand, within chpB at 4446.910 kb on + strand, within chpB at 4446.916 kb on - strand, within chpB at 4446.945 kb on + strand, within chpB at 4446.949 kb on - strand, within chpB at 4446.960 kb on + strand, within chpB at 4447.006 kb on - strand, within chpB at 4447.006 kb on - strand, within chpB at 4447.057 kb on + strand at 4447.060 kb on + strand at 4447.060 kb on + strand at 4447.779 kb on + strand at 4447.787 kb on + strand at 4447.792 kb on + strand at 4447.795 kb on - strand at 4447.795 kb on - strand at 4447.797 kb on + strand at 4447.970 kb on + strand at 4447.978 kb on - strand at 4447.983 kb on - strand at 4447.983 kb on - strand at 4448.010 kb on + strand at 4448.039 kb on + strand at 4448.039 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB remove 4,445,736 + +1.8 4,445,737 + -1.5 4,445,737 + -1.4 4,445,770 - -2.3 4,445,794 + +0.2 4,445,806 + -0.7 4,445,806 + +0.7 4,445,806 + -0.1 4,445,836 + -3.4 4,445,836 + -1.0 4,445,859 - -0.7 4,445,866 + -0.1 4,445,866 + -3.3 4,445,962 + ytfP b4222 0.13 +0.4 4,445,962 + ytfP b4222 0.13 -0.1 4,446,046 - ytfP b4222 0.38 +0.5 4,446,131 + ytfP b4222 0.63 +1.3 4,446,166 + ytfP b4222 0.73 +0.2 4,446,166 + ytfP b4222 0.73 -3.3 4,446,166 + ytfP b4222 0.73 +0.5 4,446,176 + ytfP b4222 0.76 -1.2 4,446,176 - ytfP b4222 0.76 -2.8 4,446,176 - ytfP b4222 0.76 -3.2 4,446,176 - ytfP b4222 0.76 -0.4 4,446,184 - ytfP b4222 0.78 +0.0 4,446,184 - ytfP b4222 0.78 -0.0 4,446,240 + -0.9 4,446,259 + +1.3 4,446,260 + -5.1 4,446,272 - -0.4 4,446,273 - +1.2 4,446,273 - -2.7 4,446,375 - -4.2 4,446,454 + -0.9 4,446,454 + -0.5 4,446,496 + chpS b4224 0.10 +0.0 4,446,501 + chpS b4224 0.12 +1.1 4,446,501 + chpS b4224 0.12 +0.4 4,446,509 - chpS b4224 0.15 +0.5 4,446,509 - chpS b4224 0.15 +1.8 4,446,514 - chpS b4224 0.17 -3.5 4,446,559 + chpS b4224 0.35 -0.8 4,446,569 - chpS b4224 0.39 +1.4 4,446,569 - chpS b4224 0.39 +1.0 4,446,646 - chpS b4224 0.70 +1.7 4,446,652 - chpS b4224 0.72 +1.3 4,446,746 + +0.8 4,446,777 + chpB b4225 0.18 +0.2 4,446,787 + chpB b4225 0.21 -0.6 4,446,787 + chpB b4225 0.21 -1.6 4,446,787 - chpB b4225 0.21 +0.1 4,446,787 - chpB b4225 0.21 -1.2 4,446,794 + chpB b4225 0.23 +0.1 4,446,910 + chpB b4225 0.56 -1.6 4,446,916 - chpB b4225 0.57 +0.3 4,446,945 + chpB b4225 0.66 -2.9 4,446,949 - chpB b4225 0.67 -0.5 4,446,960 + chpB b4225 0.70 -1.0 4,447,006 - chpB b4225 0.83 +1.6 4,447,006 - chpB b4225 0.83 -1.5 4,447,057 + -1.1 4,447,060 + +0.5 4,447,060 + +2.4 4,447,779 + -2.8 4,447,787 + +1.1 4,447,792 + +0.2 4,447,795 - -4.1 4,447,795 - +0.6 4,447,797 + -3.6 4,447,970 + +1.5 4,447,978 - +0.5 4,447,983 - +2.1 4,447,983 - +2.0 4,448,010 + +0.6 4,448,039 + -2.1 4,448,039 - +0.2
Or see this region's nucleotide sequence