Experiment: Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hemE and nfi are separated by 9 nucleotides nfi and yjaG are separated by 42 nucleotides yjaG and hupA are separated by 186 nucleotides
b3997: hemE - uroporphyrinogen decarboxylase (NCBI), at 4,195,739 to 4,196,803
hemE
b3998: nfi - endonuclease V (deoxyinosine 3'endoduclease) (VIMSS), at 4,196,813 to 4,197,484
nfi
b3999: yjaG - hypothetical protein (NCBI), at 4,197,527 to 4,198,117
yjaG
b4000: hupA - HU, DNA-binding transcriptional regulator, alpha subunit (NCBI), at 4,198,304 to 4,198,576
hupA
Position (kb)
4196
4197
4198 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4196.940 kb on - strand, within nfi at 4196.973 kb on - strand, within nfi at 4197.077 kb on + strand, within nfi at 4197.155 kb on - strand, within nfi at 4197.274 kb on - strand, within nfi at 4197.274 kb on - strand, within nfi at 4197.291 kb on + strand, within nfi at 4197.291 kb on + strand, within nfi at 4197.316 kb on + strand, within nfi at 4197.331 kb on + strand, within nfi at 4197.353 kb on + strand, within nfi at 4197.353 kb on + strand, within nfi at 4197.353 kb on + strand, within nfi at 4197.361 kb on - strand, within nfi at 4197.361 kb on - strand, within nfi at 4197.383 kb on - strand, within nfi at 4197.383 kb on - strand, within nfi at 4197.436 kb on - strand at 4197.436 kb on - strand at 4197.471 kb on - strand at 4197.593 kb on - strand, within yjaG at 4197.608 kb on + strand, within yjaG at 4197.618 kb on - strand, within yjaG at 4197.618 kb on - strand, within yjaG at 4197.733 kb on - strand, within yjaG at 4197.855 kb on - strand, within yjaG at 4197.855 kb on - strand, within yjaG at 4197.871 kb on + strand, within yjaG at 4197.879 kb on - strand, within yjaG at 4197.911 kb on + strand, within yjaG at 4197.911 kb on + strand, within yjaG at 4197.983 kb on - strand, within yjaG at 4198.028 kb on + strand, within yjaG at 4198.028 kb on + strand, within yjaG at 4198.070 kb on - strand at 4198.162 kb on + strand at 4198.235 kb on - strand at 4198.245 kb on - strand at 4198.250 kb on - strand at 4198.266 kb on + strand at 4198.287 kb on - strand at 4198.287 kb on - strand at 4198.359 kb on - strand, within hupA at 4198.359 kb on - strand, within hupA at 4198.380 kb on + strand, within hupA at 4198.380 kb on + strand, within hupA at 4198.388 kb on - strand, within hupA at 4198.409 kb on + strand, within hupA at 4198.409 kb on + strand, within hupA at 4198.425 kb on + strand, within hupA at 4198.443 kb on - strand, within hupA at 4198.457 kb on - strand, within hupA
Per-strain Table
Position Strand Gene LocusTag Fraction Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB remove 4,196,940 - nfi b3998 0.19 -0.9 4,196,973 - nfi b3998 0.24 -2.6 4,197,077 + nfi b3998 0.39 -1.2 4,197,155 - nfi b3998 0.51 -1.0 4,197,274 - nfi b3998 0.69 -1.3 4,197,274 - nfi b3998 0.69 -2.3 4,197,291 + nfi b3998 0.71 -3.2 4,197,291 + nfi b3998 0.71 +0.3 4,197,316 + nfi b3998 0.75 -1.3 4,197,331 + nfi b3998 0.77 -0.6 4,197,353 + nfi b3998 0.80 -0.9 4,197,353 + nfi b3998 0.80 -0.8 4,197,353 + nfi b3998 0.80 -0.5 4,197,361 - nfi b3998 0.82 -1.6 4,197,361 - nfi b3998 0.82 -0.2 4,197,383 - nfi b3998 0.85 +0.1 4,197,383 - nfi b3998 0.85 -2.7 4,197,436 - -2.4 4,197,436 - -0.3 4,197,471 - +0.1 4,197,593 - yjaG b3999 0.11 -3.8 4,197,608 + yjaG b3999 0.14 -0.7 4,197,618 - yjaG b3999 0.15 -1.9 4,197,618 - yjaG b3999 0.15 -1.0 4,197,733 - yjaG b3999 0.35 -0.4 4,197,855 - yjaG b3999 0.55 -2.8 4,197,855 - yjaG b3999 0.55 -2.3 4,197,871 + yjaG b3999 0.58 -1.7 4,197,879 - yjaG b3999 0.60 -0.7 4,197,911 + yjaG b3999 0.65 -0.9 4,197,911 + yjaG b3999 0.65 +0.2 4,197,983 - yjaG b3999 0.77 -1.6 4,198,028 + yjaG b3999 0.85 -1.8 4,198,028 + yjaG b3999 0.85 -0.0 4,198,070 - -1.0 4,198,162 + +1.8 4,198,235 - -1.3 4,198,245 - -0.1 4,198,250 - -2.2 4,198,266 + -0.4 4,198,287 - -0.3 4,198,287 - -0.5 4,198,359 - hupA b4000 0.20 +2.4 4,198,359 - hupA b4000 0.20 -3.4 4,198,380 + hupA b4000 0.28 +0.2 4,198,380 + hupA b4000 0.28 -2.2 4,198,388 - hupA b4000 0.31 -1.4 4,198,409 + hupA b4000 0.38 -1.2 4,198,409 + hupA b4000 0.38 -0.1 4,198,425 + hupA b4000 0.44 -2.2 4,198,443 - hupA b4000 0.51 +0.6 4,198,457 - hupA b4000 0.56 -1.8
Or see this region's nucleotide sequence