Experiment: Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt napA and napD overlap by 4 nucleotides napD and napF overlap by 11 nucleotides napF and yojO are separated by 108 nucleotides yojO and eco are separated by 134 nucleotides
b2206: napA - nitrate reductase, periplasmic, large subunit (NCBI), at 2,298,289 to 2,300,775
napA
b2207: napD - assembly protein for periplasmic nitrate reductase (NCBI), at 2,300,772 to 2,301,035
napD
b2208: napF - ferredoxin-type protein, predicted role in electron transfer to periplasmic nitrate reductase (NapA) (NCBI), at 2,301,025 to 2,301,519
napF
b4604: yojO - hypothetical protein (RefSeq), at 2,301,628 to 2,301,792
yojO
b2209: eco - ecotin precursor (NCBI), at 2,301,927 to 2,302,415
eco
Position (kb)
2300
2301
2302 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2299.817 kb on - strand, within napA at 2299.859 kb on + strand, within napA at 2300.072 kb on + strand, within napA at 2300.072 kb on + strand, within napA at 2300.114 kb on + strand, within napA at 2300.239 kb on + strand, within napA at 2300.240 kb on - strand, within napA at 2300.245 kb on - strand, within napA at 2300.338 kb on + strand, within napA at 2300.365 kb on + strand, within napA at 2300.365 kb on + strand, within napA at 2300.421 kb on - strand, within napA at 2300.421 kb on - strand, within napA at 2300.605 kb on + strand at 2300.832 kb on + strand, within napD at 2300.841 kb on + strand, within napD at 2300.865 kb on - strand, within napD at 2300.965 kb on + strand, within napD at 2301.037 kb on + strand at 2301.037 kb on + strand at 2301.060 kb on + strand at 2301.060 kb on + strand at 2301.084 kb on + strand, within napF at 2301.183 kb on - strand, within napF at 2301.210 kb on - strand, within napF at 2301.210 kb on - strand, within napF at 2301.256 kb on - strand, within napF at 2301.257 kb on - strand, within napF at 2301.286 kb on + strand, within napF at 2301.415 kb on - strand, within napF at 2301.496 kb on - strand at 2301.620 kb on + strand at 2301.874 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB remove 2,299,817 - napA b2206 0.61 -2.1 2,299,859 + napA b2206 0.63 -2.1 2,300,072 + napA b2206 0.72 +0.7 2,300,072 + napA b2206 0.72 +0.9 2,300,114 + napA b2206 0.73 +1.9 2,300,239 + napA b2206 0.78 +2.0 2,300,240 - napA b2206 0.78 -0.5 2,300,245 - napA b2206 0.79 +1.0 2,300,338 + napA b2206 0.82 +1.2 2,300,365 + napA b2206 0.83 -0.1 2,300,365 + napA b2206 0.83 +0.7 2,300,421 - napA b2206 0.86 -1.1 2,300,421 - napA b2206 0.86 +0.1 2,300,605 + -0.1 2,300,832 + napD b2207 0.23 +1.4 2,300,841 + napD b2207 0.26 -1.4 2,300,865 - napD b2207 0.35 +0.0 2,300,965 + napD b2207 0.73 +3.0 2,301,037 + -0.8 2,301,037 + -3.2 2,301,060 + -0.3 2,301,060 + -0.4 2,301,084 + napF b2208 0.12 +1.1 2,301,183 - napF b2208 0.32 -1.5 2,301,210 - napF b2208 0.37 -2.8 2,301,210 - napF b2208 0.37 -1.0 2,301,256 - napF b2208 0.47 +0.9 2,301,257 - napF b2208 0.47 +0.4 2,301,286 + napF b2208 0.53 +1.0 2,301,415 - napF b2208 0.79 +0.4 2,301,496 - +0.3 2,301,620 + +1.3 2,301,874 + -0.4
Or see this region's nucleotide sequence