Strain Fitness in Escherichia coli BW25113 around b1845

Experiment: Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyobB and exoX are separated by 23 nucleotidesexoX and ptrB overlap by 4 nucleotidesptrB and yebE are separated by 208 nucleotides b1843: yobB - hypothetical protein (NCBI), at 1,923,464 to 1,924,120 yobB b1844: exoX - exodeoxyribonuclease X (NCBI), at 1,924,144 to 1,924,806 exoX b1845: ptrB - protease II (NCBI), at 1,924,803 to 1,926,863 ptrB b1846: yebE - hypothetical protein (NCBI), at 1,927,072 to 1,927,731 yebE Position (kb) 1924 1925 1926 1927Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 1923.831 kb on - strand, within yobBat 1923.840 kb on - strand, within yobBat 1923.840 kb on - strand, within yobBat 1923.846 kb on + strand, within yobBat 1923.995 kb on + strand, within yobBat 1924.003 kb on - strand, within yobBat 1924.274 kb on + strand, within exoXat 1924.274 kb on + strand, within exoXat 1924.274 kb on - strand, within exoXat 1924.309 kb on - strand, within exoXat 1924.361 kb on - strand, within exoXat 1924.361 kb on - strand, within exoXat 1924.423 kb on + strand, within exoXat 1924.423 kb on + strand, within exoXat 1924.471 kb on + strand, within exoXat 1924.507 kb on - strand, within exoXat 1924.609 kb on - strand, within exoXat 1924.715 kb on + strand, within exoXat 1924.770 kb on + strandat 1924.831 kb on - strandat 1924.893 kb on + strandat 1925.014 kb on - strand, within ptrBat 1925.128 kb on + strand, within ptrBat 1925.128 kb on + strand, within ptrBat 1925.602 kb on + strand, within ptrBat 1925.602 kb on + strand, within ptrBat 1925.668 kb on - strand, within ptrBat 1925.757 kb on - strand, within ptrBat 1925.785 kb on - strand, within ptrBat 1925.976 kb on + strand, within ptrBat 1925.976 kb on + strand, within ptrBat 1925.976 kb on + strand, within ptrBat 1925.984 kb on - strand, within ptrBat 1925.984 kb on - strand, within ptrBat 1926.042 kb on + strand, within ptrBat 1926.048 kb on + strand, within ptrBat 1926.056 kb on - strand, within ptrBat 1926.172 kb on + strand, within ptrBat 1926.172 kb on + strand, within ptrBat 1926.326 kb on - strand, within ptrBat 1926.403 kb on - strand, within ptrBat 1926.489 kb on - strand, within ptrBat 1926.492 kb on + strand, within ptrBat 1926.492 kb on + strand, within ptrBat 1926.531 kb on + strand, within ptrBat 1926.554 kb on - strand, within ptrBat 1926.554 kb on - strand, within ptrBat 1926.593 kb on + strand, within ptrBat 1926.750 kb on + strandat 1926.750 kb on + strandat 1927.234 kb on + strand, within yebEat 1927.234 kb on + strand, within yebEat 1927.267 kb on + strand, within yebEat 1927.334 kb on - strand, within yebEat 1927.382 kb on - strand, within yebEat 1927.382 kb on - strand, within yebEat 1927.407 kb on + strand, within yebEat 1927.533 kb on + strand, within yebEat 1927.578 kb on + strand, within yebEat 1927.767 kb on - strandat 1927.861 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
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1,923,831 - yobB b1843 0.56 +1.1
1,923,840 - yobB b1843 0.57 -1.4
1,923,840 - yobB b1843 0.57 -3.5
1,923,846 + yobB b1843 0.58 -0.6
1,923,995 + yobB b1843 0.81 +0.0
1,924,003 - yobB b1843 0.82 -0.4
1,924,274 + exoX b1844 0.20 +0.2
1,924,274 + exoX b1844 0.20 +1.1
1,924,274 - exoX b1844 0.20 +1.2
1,924,309 - exoX b1844 0.25 +1.9
1,924,361 - exoX b1844 0.33 +0.2
1,924,361 - exoX b1844 0.33 -1.3
1,924,423 + exoX b1844 0.42 +0.9
1,924,423 + exoX b1844 0.42 -0.5
1,924,471 + exoX b1844 0.49 -1.1
1,924,507 - exoX b1844 0.55 -0.7
1,924,609 - exoX b1844 0.70 -0.0
1,924,715 + exoX b1844 0.86 -0.5
1,924,770 + +0.9
1,924,831 - -0.5
1,924,893 + -1.8
1,925,014 - ptrB b1845 0.10 -1.0
1,925,128 + ptrB b1845 0.16 -1.7
1,925,128 + ptrB b1845 0.16 -2.5
1,925,602 + ptrB b1845 0.39 -1.3
1,925,602 + ptrB b1845 0.39 +0.4
1,925,668 - ptrB b1845 0.42 -0.8
1,925,757 - ptrB b1845 0.46 -0.3
1,925,785 - ptrB b1845 0.48 -3.9
1,925,976 + ptrB b1845 0.57 -2.1
1,925,976 + ptrB b1845 0.57 -3.9
1,925,976 + ptrB b1845 0.57 -2.9
1,925,984 - ptrB b1845 0.57 +0.8
1,925,984 - ptrB b1845 0.57 +2.3
1,926,042 + ptrB b1845 0.60 -2.7
1,926,048 + ptrB b1845 0.60 +0.0
1,926,056 - ptrB b1845 0.61 +0.0
1,926,172 + ptrB b1845 0.66 -1.6
1,926,172 + ptrB b1845 0.66 +3.4
1,926,326 - ptrB b1845 0.74 -1.8
1,926,403 - ptrB b1845 0.78 +0.3
1,926,489 - ptrB b1845 0.82 +0.3
1,926,492 + ptrB b1845 0.82 -3.4
1,926,492 + ptrB b1845 0.82 -3.2
1,926,531 + ptrB b1845 0.84 -0.6
1,926,554 - ptrB b1845 0.85 -1.0
1,926,554 - ptrB b1845 0.85 -0.2
1,926,593 + ptrB b1845 0.87 +0.2
1,926,750 + -0.7
1,926,750 + -1.1
1,927,234 + yebE b1846 0.25 -0.1
1,927,234 + yebE b1846 0.25 -2.6
1,927,267 + yebE b1846 0.30 +2.3
1,927,334 - yebE b1846 0.40 -3.6
1,927,382 - yebE b1846 0.47 -3.1
1,927,382 - yebE b1846 0.47 +1.7
1,927,407 + yebE b1846 0.51 +0.6
1,927,533 + yebE b1846 0.70 -3.6
1,927,578 + yebE b1846 0.77 -1.3
1,927,767 - -1.4
1,927,861 + -0.1

Or see this region's nucleotide sequence