Experiment: Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yeaJ and yeaK are separated by 42 nucleotides yeaK and b1788 are separated by 0 nucleotides b1788 and yeaL are separated by 169 nucleotides yeaL and yeaM overlap by 44 nucleotides
b1786: yeaJ - orf, hypothetical protein (VIMSS), at 1,870,065 to 1,871,555
yeaJ
b1787: yeaK - hypothetical protein (NCBI), at 1,871,598 to 1,872,101
yeaK
b1788: b1788 - orf, hypothetical protein (VIMSS), at 1,872,102 to 1,872,206
b1788
b1789: yeaL - conserved inner membrane protein (NCBI), at 1,872,376 to 1,872,822
yeaL
b1790: yeaM - predicted DNA-binding transcriptional regulator (NCBI), at 1,872,779 to 1,873,600
yeaM
Position (kb)
1871
1872
1873 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1870.602 kb on + strand, within yeaJ at 1870.602 kb on + strand, within yeaJ at 1870.604 kb on - strand, within yeaJ at 1870.609 kb on + strand, within yeaJ at 1870.609 kb on + strand, within yeaJ at 1870.617 kb on - strand, within yeaJ at 1870.619 kb on - strand, within yeaJ at 1870.627 kb on + strand, within yeaJ at 1870.683 kb on - strand, within yeaJ at 1870.683 kb on - strand, within yeaJ at 1870.711 kb on + strand, within yeaJ at 1870.742 kb on + strand, within yeaJ at 1870.742 kb on + strand, within yeaJ at 1870.742 kb on + strand, within yeaJ at 1870.748 kb on - strand, within yeaJ at 1870.808 kb on + strand, within yeaJ at 1870.808 kb on + strand, within yeaJ at 1870.819 kb on - strand, within yeaJ at 1870.823 kb on - strand, within yeaJ at 1870.838 kb on - strand, within yeaJ at 1870.843 kb on + strand, within yeaJ at 1870.849 kb on + strand, within yeaJ at 1870.861 kb on - strand, within yeaJ at 1870.867 kb on - strand, within yeaJ at 1870.872 kb on - strand, within yeaJ at 1870.887 kb on + strand, within yeaJ at 1870.890 kb on + strand, within yeaJ at 1870.890 kb on - strand, within yeaJ at 1870.925 kb on - strand, within yeaJ at 1870.932 kb on + strand, within yeaJ at 1870.939 kb on + strand, within yeaJ at 1870.941 kb on + strand, within yeaJ at 1870.948 kb on - strand, within yeaJ at 1871.045 kb on + strand, within yeaJ at 1871.045 kb on + strand, within yeaJ at 1871.047 kb on + strand, within yeaJ at 1871.047 kb on + strand, within yeaJ at 1871.052 kb on + strand, within yeaJ at 1871.085 kb on - strand, within yeaJ at 1871.153 kb on - strand, within yeaJ at 1871.217 kb on - strand, within yeaJ at 1871.218 kb on + strand, within yeaJ at 1871.253 kb on - strand, within yeaJ at 1871.312 kb on + strand, within yeaJ at 1871.335 kb on + strand, within yeaJ at 1871.390 kb on + strand, within yeaJ at 1871.396 kb on - strand, within yeaJ at 1871.414 kb on + strand at 1871.414 kb on + strand at 1871.436 kb on + strand at 1871.483 kb on + strand at 1871.495 kb on + strand at 1871.495 kb on + strand at 1871.502 kb on - strand at 1871.534 kb on + strand at 1871.534 kb on + strand at 1871.534 kb on + strand at 1871.534 kb on + strand at 1871.551 kb on + strand at 1871.551 kb on + strand at 1871.581 kb on + strand at 1871.581 kb on + strand at 1871.734 kb on + strand, within yeaK at 1871.741 kb on - strand, within yeaK at 1871.746 kb on - strand, within yeaK at 1871.748 kb on + strand, within yeaK at 1871.748 kb on + strand, within yeaK at 1871.754 kb on - strand, within yeaK at 1871.754 kb on - strand, within yeaK at 1871.759 kb on + strand, within yeaK at 1871.790 kb on - strand, within yeaK at 1871.804 kb on - strand, within yeaK at 1871.823 kb on - strand, within yeaK at 1871.909 kb on - strand, within yeaK at 1871.909 kb on - strand, within yeaK at 1871.978 kb on - strand, within yeaK at 1872.285 kb on - strand at 1872.399 kb on - strand at 1872.443 kb on - strand, within yeaL at 1872.584 kb on + strand, within yeaL at 1872.606 kb on - strand, within yeaL at 1872.672 kb on - strand, within yeaL at 1872.672 kb on - strand, within yeaL at 1872.680 kb on + strand, within yeaL at 1872.680 kb on + strand, within yeaL
Per-strain Table
Position Strand Gene LocusTag Fraction Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB remove 1,870,602 + yeaJ b1786 0.36 +2.3 1,870,602 + yeaJ b1786 0.36 -3.1 1,870,604 - yeaJ b1786 0.36 +2.2 1,870,609 + yeaJ b1786 0.36 -1.5 1,870,609 + yeaJ b1786 0.36 -2.7 1,870,617 - yeaJ b1786 0.37 -0.8 1,870,619 - yeaJ b1786 0.37 +0.2 1,870,627 + yeaJ b1786 0.38 +3.3 1,870,683 - yeaJ b1786 0.41 +0.2 1,870,683 - yeaJ b1786 0.41 +1.2 1,870,711 + yeaJ b1786 0.43 +0.8 1,870,742 + yeaJ b1786 0.45 -2.4 1,870,742 + yeaJ b1786 0.45 -2.3 1,870,742 + yeaJ b1786 0.45 -3.0 1,870,748 - yeaJ b1786 0.46 -0.4 1,870,808 + yeaJ b1786 0.50 +0.2 1,870,808 + yeaJ b1786 0.50 +1.2 1,870,819 - yeaJ b1786 0.51 -1.7 1,870,823 - yeaJ b1786 0.51 +1.5 1,870,838 - yeaJ b1786 0.52 -0.1 1,870,843 + yeaJ b1786 0.52 -0.2 1,870,849 + yeaJ b1786 0.53 +0.6 1,870,861 - yeaJ b1786 0.53 +0.1 1,870,867 - yeaJ b1786 0.54 +1.6 1,870,872 - yeaJ b1786 0.54 +2.2 1,870,887 + yeaJ b1786 0.55 +0.7 1,870,890 + yeaJ b1786 0.55 -3.4 1,870,890 - yeaJ b1786 0.55 +0.5 1,870,925 - yeaJ b1786 0.58 -2.2 1,870,932 + yeaJ b1786 0.58 -0.6 1,870,939 + yeaJ b1786 0.59 -0.4 1,870,941 + yeaJ b1786 0.59 -3.3 1,870,948 - yeaJ b1786 0.59 -2.4 1,871,045 + yeaJ b1786 0.66 -1.7 1,871,045 + yeaJ b1786 0.66 +1.9 1,871,047 + yeaJ b1786 0.66 +0.8 1,871,047 + yeaJ b1786 0.66 -1.2 1,871,052 + yeaJ b1786 0.66 +0.4 1,871,085 - yeaJ b1786 0.68 +0.6 1,871,153 - yeaJ b1786 0.73 +0.6 1,871,217 - yeaJ b1786 0.77 +1.2 1,871,218 + yeaJ b1786 0.77 +1.9 1,871,253 - yeaJ b1786 0.80 -2.4 1,871,312 + yeaJ b1786 0.84 -1.0 1,871,335 + yeaJ b1786 0.85 -3.3 1,871,390 + yeaJ b1786 0.89 +1.2 1,871,396 - yeaJ b1786 0.89 +0.4 1,871,414 + -1.6 1,871,414 + -1.7 1,871,436 + +1.7 1,871,483 + -2.2 1,871,495 + -2.5 1,871,495 + -3.2 1,871,502 - -0.2 1,871,534 + -2.1 1,871,534 + +1.2 1,871,534 + -1.0 1,871,534 + -0.9 1,871,551 + +0.9 1,871,551 + +1.6 1,871,581 + -0.2 1,871,581 + -0.4 1,871,734 + yeaK b1787 0.27 -2.4 1,871,741 - yeaK b1787 0.28 -1.9 1,871,746 - yeaK b1787 0.29 -0.0 1,871,748 + yeaK b1787 0.30 -0.1 1,871,748 + yeaK b1787 0.30 -3.0 1,871,754 - yeaK b1787 0.31 -1.9 1,871,754 - yeaK b1787 0.31 -1.3 1,871,759 + yeaK b1787 0.32 -0.1 1,871,790 - yeaK b1787 0.38 -0.8 1,871,804 - yeaK b1787 0.41 -1.0 1,871,823 - yeaK b1787 0.45 +2.2 1,871,909 - yeaK b1787 0.62 -2.8 1,871,909 - yeaK b1787 0.62 -1.5 1,871,978 - yeaK b1787 0.75 -0.7 1,872,285 - -1.4 1,872,399 - -0.0 1,872,443 - yeaL b1789 0.15 -0.4 1,872,584 + yeaL b1789 0.47 +0.1 1,872,606 - yeaL b1789 0.51 -0.6 1,872,672 - yeaL b1789 0.66 -0.6 1,872,672 - yeaL b1789 0.66 -1.6 1,872,680 + yeaL b1789 0.68 -2.5 1,872,680 + yeaL b1789 0.68 -0.7
Or see this region's nucleotide sequence