Experiment: Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt chaA and chaB are separated by 269 nucleotides chaB and chaC are separated by 157 nucleotides chaC and ychN are separated by 43 nucleotides ychN and ychO are separated by 184 nucleotides
b1216: chaA - calcium/sodium:proton antiporter (NCBI), at 1,269,972 to 1,271,072
chaA
b1217: chaB - cation transport regulator (NCBI), at 1,271,342 to 1,271,572
chaB
b1218: chaC - regulatory protein for cation transport (RefSeq), at 1,271,730 to 1,272,425
chaC
b1219: ychN - hypothetical protein (NCBI), at 1,272,469 to 1,272,822
ychN
b1220: ychO - predicted invasin (RefSeq), at 1,273,007 to 1,274,401
ychO
Position (kb)
1271
1272
1273 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1270.750 kb on - strand, within chaA at 1270.755 kb on - strand, within chaA at 1270.780 kb on - strand, within chaA at 1270.790 kb on - strand, within chaA at 1270.790 kb on - strand, within chaA at 1270.878 kb on + strand, within chaA at 1270.878 kb on + strand, within chaA at 1270.972 kb on - strand at 1270.972 kb on - strand at 1270.977 kb on - strand at 1270.986 kb on + strand at 1270.988 kb on + strand at 1270.994 kb on + strand at 1270.994 kb on + strand at 1271.045 kb on - strand at 1271.054 kb on + strand at 1271.074 kb on + strand at 1271.074 kb on + strand at 1271.083 kb on - strand at 1271.083 kb on - strand at 1271.145 kb on + strand at 1271.248 kb on + strand at 1271.254 kb on - strand at 1271.256 kb on - strand at 1271.342 kb on - strand at 1271.347 kb on + strand at 1271.381 kb on + strand, within chaB at 1271.381 kb on + strand, within chaB at 1271.475 kb on - strand, within chaB at 1271.723 kb on + strand at 1271.892 kb on + strand, within chaC at 1271.892 kb on + strand, within chaC at 1271.932 kb on - strand, within chaC at 1272.143 kb on - strand, within chaC at 1272.159 kb on - strand, within chaC at 1272.288 kb on - strand, within chaC at 1272.522 kb on - strand, within ychN at 1272.532 kb on - strand, within ychN at 1272.581 kb on - strand, within ychN at 1272.615 kb on - strand, within ychN at 1272.714 kb on + strand, within ychN at 1272.787 kb on - strand at 1272.787 kb on - strand at 1273.039 kb on + strand at 1273.305 kb on + strand, within ychO at 1273.328 kb on - strand, within ychO at 1273.328 kb on - strand, within ychO
Per-strain Table
Position Strand Gene LocusTag Fraction Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB remove 1,270,750 - chaA b1216 0.71 +0.7 1,270,755 - chaA b1216 0.71 +0.2 1,270,780 - chaA b1216 0.73 -0.7 1,270,790 - chaA b1216 0.74 -2.6 1,270,790 - chaA b1216 0.74 +0.2 1,270,878 + chaA b1216 0.82 -1.7 1,270,878 + chaA b1216 0.82 -1.6 1,270,972 - -1.3 1,270,972 - +1.0 1,270,977 - -3.1 1,270,986 + +0.6 1,270,988 + +1.9 1,270,994 + -0.9 1,270,994 + -2.4 1,271,045 - +0.4 1,271,054 + -0.6 1,271,074 + -0.4 1,271,074 + -1.4 1,271,083 - +2.0 1,271,083 - -0.4 1,271,145 + -1.0 1,271,248 + -0.6 1,271,254 - -0.9 1,271,256 - -2.0 1,271,342 - +0.8 1,271,347 + -2.3 1,271,381 + chaB b1217 0.17 -2.3 1,271,381 + chaB b1217 0.17 -1.2 1,271,475 - chaB b1217 0.58 -0.5 1,271,723 + -0.1 1,271,892 + chaC b1218 0.23 -0.4 1,271,892 + chaC b1218 0.23 -3.9 1,271,932 - chaC b1218 0.29 -2.1 1,272,143 - chaC b1218 0.59 +0.4 1,272,159 - chaC b1218 0.62 -2.6 1,272,288 - chaC b1218 0.80 +1.4 1,272,522 - ychN b1219 0.15 -2.0 1,272,532 - ychN b1219 0.18 -1.5 1,272,581 - ychN b1219 0.32 +0.6 1,272,615 - ychN b1219 0.41 +0.7 1,272,714 + ychN b1219 0.69 +1.9 1,272,787 - -3.8 1,272,787 - -0.1 1,273,039 + -1.9 1,273,305 + ychO b1220 0.21 +0.3 1,273,328 - ychO b1220 0.23 +0.0 1,273,328 - ychO b1220 0.23 +0.2
Or see this region's nucleotide sequence