Experiment: Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybgJ and ybgK overlap by 7 nucleotides ybgK and ybgL overlap by 11 nucleotides ybgL and nei are separated by 35 nucleotides nei and abrB overlap by 4 nucleotides
b0711: ybgJ - predicted enzyme subunit (NCBI), at 742,816 to 743,472
ybgJ
b0712: ybgK - predicted enzyme subunit (NCBI), at 743,466 to 744,398
ybgK
b0713: ybgL - hypothetical protein (NCBI), at 744,388 to 745,122
ybgL
b0714: nei - endonuclease VIII/ 5-formyluracil/5-hydroxymethyluracil DNA glycosylase (NCBI), at 745,158 to 745,949
nei
b0715: abrB - putative transport protein (VIMSS), at 745,946 to 746,992
abrB
Position (kb) 744
745
746 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 743.461 kb on + strand at 743.489 kb on + strand at 743.492 kb on + strand at 743.492 kb on + strand at 743.492 kb on + strand at 743.500 kb on - strand at 743.500 kb on - strand at 743.504 kb on + strand at 743.515 kb on - strand at 743.538 kb on + strand at 743.538 kb on + strand at 743.539 kb on + strand at 743.539 kb on + strand at 743.595 kb on - strand, within ybgK at 743.595 kb on - strand, within ybgK at 743.642 kb on + strand, within ybgK at 743.799 kb on + strand, within ybgK at 743.865 kb on - strand, within ybgK at 743.880 kb on + strand, within ybgK at 743.880 kb on + strand, within ybgK at 743.888 kb on - strand, within ybgK at 743.920 kb on - strand, within ybgK at 743.946 kb on - strand, within ybgK at 743.956 kb on + strand, within ybgK at 743.956 kb on + strand, within ybgK at 744.039 kb on + strand, within ybgK at 744.039 kb on + strand, within ybgK at 744.080 kb on + strand, within ybgK at 744.080 kb on + strand, within ybgK at 744.152 kb on + strand, within ybgK at 744.271 kb on - strand, within ybgK at 744.423 kb on - strand at 744.662 kb on - strand, within ybgL at 744.662 kb on - strand, within ybgL at 744.711 kb on + strand, within ybgL at 744.766 kb on + strand, within ybgL at 744.832 kb on - strand, within ybgL at 744.978 kb on - strand, within ybgL at 744.978 kb on - strand, within ybgL at 745.140 kb on - strand at 745.148 kb on + strand at 745.194 kb on + strand at 745.194 kb on + strand at 745.286 kb on + strand, within nei at 745.297 kb on + strand, within nei at 745.297 kb on + strand, within nei at 745.358 kb on - strand, within nei at 745.358 kb on - strand, within nei at 745.419 kb on + strand, within nei at 745.689 kb on - strand, within nei at 745.991 kb on + strand at 746.028 kb on - strand at 746.035 kb on - strand at 746.088 kb on + strand, within abrB at 746.122 kb on - strand, within abrB
Per-strain Table
Position Strand Gene LocusTag Fraction Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB remove 743,461 + +0.7 743,489 + +0.1 743,492 + -0.7 743,492 + -0.4 743,492 + -3.3 743,500 - +0.8 743,500 - +1.0 743,504 + -0.3 743,515 - +0.7 743,538 + -1.1 743,538 + -2.2 743,539 + -2.8 743,539 + -3.5 743,595 - ybgK b0712 0.14 -1.3 743,595 - ybgK b0712 0.14 +1.0 743,642 + ybgK b0712 0.19 -0.4 743,799 + ybgK b0712 0.36 -0.6 743,865 - ybgK b0712 0.43 -2.4 743,880 + ybgK b0712 0.44 +0.9 743,880 + ybgK b0712 0.44 +0.6 743,888 - ybgK b0712 0.45 -0.9 743,920 - ybgK b0712 0.49 -0.7 743,946 - ybgK b0712 0.51 -1.1 743,956 + ybgK b0712 0.53 +1.1 743,956 + ybgK b0712 0.53 -0.2 744,039 + ybgK b0712 0.61 +0.9 744,039 + ybgK b0712 0.61 -2.8 744,080 + ybgK b0712 0.66 -0.3 744,080 + ybgK b0712 0.66 -0.3 744,152 + ybgK b0712 0.74 -4.0 744,271 - ybgK b0712 0.86 -3.4 744,423 - +0.3 744,662 - ybgL b0713 0.37 -1.3 744,662 - ybgL b0713 0.37 -0.5 744,711 + ybgL b0713 0.44 -0.9 744,766 + ybgL b0713 0.51 -3.1 744,832 - ybgL b0713 0.60 -0.4 744,978 - ybgL b0713 0.80 -0.1 744,978 - ybgL b0713 0.80 -1.2 745,140 - -3.1 745,148 + -3.3 745,194 + -0.3 745,194 + -2.2 745,286 + nei b0714 0.16 +0.4 745,297 + nei b0714 0.18 -1.3 745,297 + nei b0714 0.18 -0.4 745,358 - nei b0714 0.25 -0.2 745,358 - nei b0714 0.25 -2.6 745,419 + nei b0714 0.33 -0.7 745,689 - nei b0714 0.67 +1.3 745,991 + -0.2 746,028 - +0.4 746,035 - +2.8 746,088 + abrB b0715 0.14 +1.6 746,122 - abrB b0715 0.17 -0.6
Or see this region's nucleotide sequence