Experiment: Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt citE and citD overlap by 4 nucleotides citD and citC are separated by 14 nucleotides citC and citA are separated by 378 nucleotides
b0616: citE - citrate lyase beta chain (acyl lyase subunit) (VIMSS), at 648,805 to 649,713
citE
b0617: citD - citrate lyase, acyl carrier (gamma) subunit (NCBI), at 649,710 to 650,006
citD
b0618: citC - citrate lyase synthetase (citrate (pro-3S)-lyase ligase (VIMSS), at 650,021 to 651,079
citC
b0619: citA - sensory histidine kinase in two-component regulatory system with citB (NCBI), at 651,458 to 653,116
citA
Position (kb) 650
651
652 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 649.122 kb on - strand, within citE at 649.238 kb on - strand, within citE at 649.238 kb on - strand, within citE at 649.327 kb on - strand, within citE at 649.479 kb on + strand, within citE at 649.540 kb on - strand, within citE at 649.569 kb on + strand, within citE at 649.595 kb on - strand, within citE at 649.595 kb on - strand, within citE at 649.607 kb on + strand, within citE at 649.642 kb on + strand at 649.665 kb on - strand at 649.828 kb on - strand, within citD at 649.828 kb on - strand, within citD at 649.831 kb on - strand, within citD at 649.947 kb on - strand, within citD at 650.199 kb on + strand, within citC at 650.221 kb on + strand, within citC at 650.310 kb on - strand, within citC at 650.326 kb on + strand, within citC at 650.332 kb on - strand, within citC at 650.332 kb on - strand at 650.456 kb on - strand, within citC at 650.577 kb on + strand, within citC at 650.733 kb on - strand, within citC at 650.794 kb on + strand, within citC at 650.808 kb on + strand, within citC at 650.808 kb on + strand, within citC at 650.808 kb on + strand, within citC at 650.816 kb on - strand, within citC at 650.818 kb on + strand, within citC at 650.818 kb on + strand, within citC at 650.818 kb on + strand, within citC at 650.858 kb on + strand, within citC at 650.860 kb on - strand, within citC at 650.863 kb on + strand, within citC at 650.863 kb on + strand, within citC at 650.863 kb on + strand, within citC at 650.885 kb on + strand, within citC at 650.885 kb on + strand, within citC at 650.885 kb on + strand, within citC at 650.888 kb on + strand, within citC at 650.888 kb on + strand, within citC at 650.888 kb on - strand, within citC at 650.955 kb on + strand, within citC at 650.955 kb on + strand, within citC at 650.962 kb on + strand, within citC at 650.966 kb on + strand, within citC at 650.966 kb on + strand, within citC at 650.978 kb on + strand at 650.978 kb on + strand at 650.988 kb on + strand at 650.988 kb on + strand at 650.992 kb on + strand at 650.992 kb on + strand at 651.085 kb on + strand at 651.218 kb on + strand at 651.525 kb on - strand at 651.551 kb on - strand at 651.606 kb on - strand at 651.606 kb on - strand at 651.611 kb on + strand at 651.616 kb on + strand at 651.622 kb on - strand at 651.622 kb on - strand at 651.629 kb on - strand, within citA at 651.629 kb on - strand, within citA at 651.657 kb on + strand, within citA at 651.661 kb on - strand, within citA at 651.663 kb on - strand, within citA at 651.669 kb on + strand, within citA at 651.671 kb on - strand, within citA at 651.671 kb on - strand, within citA at 651.677 kb on - strand, within citA at 651.690 kb on + strand, within citA at 651.711 kb on + strand, within citA at 651.711 kb on + strand, within citA at 651.784 kb on - strand, within citA at 651.810 kb on + strand, within citA at 651.810 kb on + strand, within citA at 651.818 kb on - strand, within citA at 651.818 kb on - strand, within citA at 651.876 kb on + strand, within citA at 651.877 kb on + strand, within citA
Per-strain Table
Position Strand Gene LocusTag Fraction Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB remove 649,122 - citE b0616 0.35 -0.6 649,238 - citE b0616 0.48 +0.7 649,238 - citE b0616 0.48 +2.3 649,327 - citE b0616 0.57 +0.4 649,479 + citE b0616 0.74 +0.1 649,540 - citE b0616 0.81 -1.8 649,569 + citE b0616 0.84 +0.1 649,595 - citE b0616 0.87 -1.9 649,595 - citE b0616 0.87 -2.3 649,607 + citE b0616 0.88 +0.0 649,642 + +1.4 649,665 - -0.1 649,828 - citD b0617 0.40 +0.8 649,828 - citD b0617 0.40 -0.1 649,831 - citD b0617 0.41 -1.5 649,947 - citD b0617 0.80 -0.3 650,199 + citC b0618 0.17 +0.3 650,221 + citC b0618 0.19 -4.3 650,310 - citC b0618 0.27 -1.5 650,326 + citC b0618 0.29 -1.6 650,332 - citC b0618 0.29 +0.4 650,332 - +1.6 650,456 - citC b0618 0.41 +1.2 650,577 + citC b0618 0.53 +0.8 650,733 - citC b0618 0.67 +3.0 650,794 + citC b0618 0.73 -1.0 650,808 + citC b0618 0.74 -0.5 650,808 + citC b0618 0.74 +2.3 650,808 + citC b0618 0.74 +2.8 650,816 - citC b0618 0.75 -3.8 650,818 + citC b0618 0.75 -2.0 650,818 + citC b0618 0.75 -2.1 650,818 + citC b0618 0.75 +3.2 650,858 + citC b0618 0.79 -2.2 650,860 - citC b0618 0.79 +0.2 650,863 + citC b0618 0.80 -2.3 650,863 + citC b0618 0.80 +2.4 650,863 + citC b0618 0.80 -0.7 650,885 + citC b0618 0.82 +0.7 650,885 + citC b0618 0.82 +2.3 650,885 + citC b0618 0.82 +4.3 650,888 + citC b0618 0.82 -1.5 650,888 + citC b0618 0.82 -2.7 650,888 - citC b0618 0.82 +2.2 650,955 + citC b0618 0.88 +0.6 650,955 + citC b0618 0.88 +2.0 650,962 + citC b0618 0.89 -0.6 650,966 + citC b0618 0.89 +4.2 650,966 + citC b0618 0.89 +2.3 650,978 + -0.1 650,978 + -1.1 650,988 + -0.8 650,988 + +0.6 650,992 + +2.2 650,992 + +2.2 651,085 + +2.2 651,218 + +2.2 651,525 - -0.4 651,551 - +2.2 651,606 - +2.2 651,606 - +2.2 651,611 + +2.2 651,616 + +2.2 651,622 - +0.2 651,622 - -0.6 651,629 - citA b0619 0.10 +0.6 651,629 - citA b0619 0.10 +2.2 651,657 + citA b0619 0.12 +2.3 651,661 - citA b0619 0.12 -0.4 651,663 - citA b0619 0.12 -0.6 651,669 + citA b0619 0.13 -0.4 651,671 - citA b0619 0.13 -2.0 651,671 - citA b0619 0.13 +0.6 651,677 - citA b0619 0.13 +1.2 651,690 + citA b0619 0.14 +2.1 651,711 + citA b0619 0.15 -2.4 651,711 + citA b0619 0.15 +1.6 651,784 - citA b0619 0.20 +1.9 651,810 + citA b0619 0.21 +0.1 651,810 + citA b0619 0.21 +2.3 651,818 - citA b0619 0.22 +1.4 651,818 - citA b0619 0.22 -2.0 651,876 + citA b0619 0.25 +1.8 651,877 + citA b0619 0.25 -0.2
Or see this region's nucleotide sequence