Experiment: Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Biofilm_growth_time=7hrs; Biofilm_growth_media=LB; Sample_type=planktonic; Outgrowth=None
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yhhZ and yrhA overlap by 4 nucleotides yrhA and insA-6 are separated by 64 nucleotides insA-6 and insB-6 overlap by 82 nucleotides insB-6 and yrhD are separated by 223 nucleotides
b3442: yhhZ - hypothetical protein (NCBI), at 3,579,886 to 3,581,064
yhhZ
b3443: yrhA - orf, hypothetical protein (VIMSS), at 3,581,061 to 3,581,441
yrhA
b3444: insA-6 - IS1 repressor protein InsA (NCBI), at 3,581,506 to 3,581,781
insA-6
b3445: insB-6 - IS1 transposase InsAB' (NCBI), at 3,581,700 to 3,582,203
insB-6
b4612: yrhD - hypothetical protein (RefSeq), at 3,582,427 to 3,582,582
yrhD
Position (kb)
3581
3582 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3580.425 kb on - strand, within yhhZ at 3580.473 kb on + strand, within yhhZ at 3581.376 kb on - strand, within yrhA at 3581.399 kb on - strand, within yrhA at 3581.399 kb on - strand, within yrhA at 3581.402 kb on + strand, within yrhA at 3581.410 kb on - strand at 3581.452 kb on - strand at 3581.509 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Biofilm_growth_time=7hrs; Biofilm_growth_media=LB; Sample_type=planktonic; Outgrowth=None remove 3,580,425 - yhhZ b3442 0.46 +2.1 3,580,473 + yhhZ b3442 0.50 +2.1 3,581,376 - yrhA b3443 0.83 -1.9 3,581,399 - yrhA b3443 0.89 -1.2 3,581,399 - yrhA b3443 0.89 -1.4 3,581,402 + yrhA b3443 0.90 +2.1 3,581,410 - +0.1 3,581,452 - -0.4 3,581,509 - +0.6
Or see this region's nucleotide sequence