Experiment: Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Biofilm_growth_time=7hrs; Biofilm_growth_media=LB; Sample_type=planktonic; Outgrowth=None
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt flhA and flhB overlap by 8 nucleotides flhB and cheZ are separated by 201 nucleotides cheZ and cheY are separated by 10 nucleotides
b1879: flhA - flagellar biosynthesis protein A (NCBI), at 1,960,996 to 1,963,074
flhA
b1880: flhB - flagellar biosynthesis protein B (NCBI), at 1,963,067 to 1,964,215
flhB
b1881: cheZ - chemotaxis regulator, protein phosphatase for CheY (NCBI), at 1,964,417 to 1,965,061
cheZ
b1882: cheY - chemotaxis regulator transmitting signal to flagellar motor component (NCBI), at 1,965,072 to 1,965,461
cheY
Position (kb)
1963
1964
1965 Strain fitness (log2 ratio)
-1
0
1 at 1962.154 kb on + strand, within flhA at 1962.257 kb on + strand, within flhA at 1962.265 kb on - strand, within flhA at 1962.265 kb on - strand, within flhA at 1962.513 kb on + strand, within flhA at 1962.513 kb on + strand, within flhA at 1962.958 kb on - strand at 1963.140 kb on - strand at 1963.140 kb on - strand at 1963.206 kb on + strand, within flhB at 1963.214 kb on - strand, within flhB at 1963.214 kb on - strand, within flhB at 1963.245 kb on + strand, within flhB at 1963.349 kb on - strand, within flhB at 1963.501 kb on - strand, within flhB at 1963.599 kb on + strand, within flhB at 1963.636 kb on - strand, within flhB at 1963.647 kb on - strand, within flhB at 1963.647 kb on - strand, within flhB at 1963.705 kb on - strand, within flhB at 1963.946 kb on + strand, within flhB at 1964.064 kb on - strand, within flhB at 1964.080 kb on + strand, within flhB at 1964.080 kb on + strand, within flhB at 1964.088 kb on + strand, within flhB at 1964.507 kb on - strand, within cheZ at 1964.609 kb on - strand, within cheZ at 1964.847 kb on - strand, within cheZ at 1964.889 kb on + strand, within cheZ
Per-strain Table
Position Strand Gene LocusTag Fraction Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Biofilm_growth_time=7hrs; Biofilm_growth_media=LB; Sample_type=planktonic; Outgrowth=None remove 1,962,154 + flhA b1879 0.56 -0.3 1,962,257 + flhA b1879 0.61 -0.2 1,962,265 - flhA b1879 0.61 +0.3 1,962,265 - flhA b1879 0.61 +0.9 1,962,513 + flhA b1879 0.73 -1.0 1,962,513 + flhA b1879 0.73 +0.3 1,962,958 - +0.8 1,963,140 - -0.3 1,963,140 - -0.3 1,963,206 + flhB b1880 0.12 -1.0 1,963,214 - flhB b1880 0.13 +0.4 1,963,214 - flhB b1880 0.13 +0.1 1,963,245 + flhB b1880 0.15 +0.0 1,963,349 - flhB b1880 0.25 +0.0 1,963,501 - flhB b1880 0.38 -0.0 1,963,599 + flhB b1880 0.46 +0.0 1,963,636 - flhB b1880 0.50 +0.4 1,963,647 - flhB b1880 0.50 +0.4 1,963,647 - flhB b1880 0.50 +0.2 1,963,705 - flhB b1880 0.56 +0.0 1,963,946 + flhB b1880 0.77 -0.7 1,964,064 - flhB b1880 0.87 +0.1 1,964,080 + flhB b1880 0.88 +0.4 1,964,080 + flhB b1880 0.88 -0.5 1,964,088 + flhB b1880 0.89 -0.1 1,964,507 - cheZ b1881 0.14 -0.6 1,964,609 - cheZ b1881 0.30 -0.3 1,964,847 - cheZ b1881 0.67 +0.3 1,964,889 + cheZ b1881 0.73 +0.1
Or see this region's nucleotide sequence