Experiment: Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Biofilm_growth_time=7hrs; Biofilm_growth_media=LB; Sample_type=planktonic; Outgrowth=None
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yneJ and yneK are separated by 77 nucleotides yneK and ydeA are separated by 149 nucleotides
b1526: yneJ - predicted DNA-binding transcriptional regulator (NCBI), at 1,612,828 to 1,613,709
yneJ
b1527: yneK - hypothetical protein (NCBI), at 1,613,787 to 1,614,902
yneK
b1528: ydeA - sugar efflux transporter (NCBI), at 1,615,052 to 1,616,242
ydeA
Position (kb)
1613
1614
1615 Strain fitness (log2 ratio)
-1
0
1 at 1612.999 kb on + strand, within yneJ at 1612.999 kb on + strand, within yneJ at 1613.337 kb on - strand, within yneJ at 1613.373 kb on + strand, within yneJ at 1613.444 kb on + strand, within yneJ at 1613.563 kb on + strand, within yneJ at 1613.695 kb on - strand at 1613.695 kb on - strand at 1613.743 kb on + strand at 1613.743 kb on + strand at 1613.750 kb on + strand at 1613.769 kb on + strand at 1613.774 kb on + strand at 1613.813 kb on + strand at 1614.197 kb on - strand, within yneK at 1614.356 kb on - strand, within yneK at 1614.567 kb on - strand, within yneK at 1614.730 kb on - strand, within yneK at 1614.730 kb on - strand, within yneK at 1614.774 kb on - strand, within yneK at 1614.810 kb on + strand at 1615.094 kb on - strand at 1615.094 kb on - strand at 1615.131 kb on - strand at 1615.131 kb on - strand at 1615.269 kb on + strand, within ydeA at 1615.269 kb on + strand, within ydeA at 1615.438 kb on + strand, within ydeA at 1615.468 kb on + strand, within ydeA at 1615.468 kb on + strand, within ydeA at 1615.572 kb on + strand, within ydeA at 1615.634 kb on - strand, within ydeA at 1615.634 kb on - strand, within ydeA at 1615.704 kb on - strand, within ydeA at 1615.741 kb on - strand, within ydeA at 1615.741 kb on - strand, within ydeA
Per-strain Table
Position Strand Gene LocusTag Fraction Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Biofilm_growth_time=7hrs; Biofilm_growth_media=LB; Sample_type=planktonic; Outgrowth=None remove 1,612,999 + yneJ b1526 0.19 +0.4 1,612,999 + yneJ b1526 0.19 +1.3 1,613,337 - yneJ b1526 0.58 -0.2 1,613,373 + yneJ b1526 0.62 +0.4 1,613,444 + yneJ b1526 0.70 +0.5 1,613,563 + yneJ b1526 0.83 -0.0 1,613,695 - -0.6 1,613,695 - +0.3 1,613,743 + +0.1 1,613,743 + -1.0 1,613,750 + +0.1 1,613,769 + -0.9 1,613,774 + -0.9 1,613,813 + +0.1 1,614,197 - yneK b1527 0.37 +1.1 1,614,356 - yneK b1527 0.51 +1.1 1,614,567 - yneK b1527 0.70 +1.1 1,614,730 - yneK b1527 0.84 -1.3 1,614,730 - yneK b1527 0.84 -0.8 1,614,774 - yneK b1527 0.88 -1.6 1,614,810 + -1.1 1,615,094 - -0.3 1,615,094 - +0.6 1,615,131 - +0.7 1,615,131 - +1.0 1,615,269 + ydeA b1528 0.18 +0.4 1,615,269 + ydeA b1528 0.18 -0.2 1,615,438 + ydeA b1528 0.32 +0.8 1,615,468 + ydeA b1528 0.35 +0.3 1,615,468 + ydeA b1528 0.35 +0.4 1,615,572 + ydeA b1528 0.44 +0.1 1,615,634 - ydeA b1528 0.49 -0.4 1,615,634 - ydeA b1528 0.49 -1.0 1,615,704 - ydeA b1528 0.55 -0.1 1,615,741 - ydeA b1528 0.58 -0.3 1,615,741 - ydeA b1528 0.58 -0.1
Or see this region's nucleotide sequence