Strain Fitness in Escherichia coli BW25113 around b3330

Experiment: Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgspF and gspG are separated by 9 nucleotidesgspG and gspH are separated by 7 nucleotidesgspH and yheH overlap by 4 nucleotidesyheH and gspJ overlap by 8 nucleotidesgspJ and gspK overlap by 8 nucleotides b3327: gspF - general secretory pathway component, cryptic (NCBI), at 3,457,839 to 3,459,035 gspF b3328: gspG - pseudopilin, cryptic, general secretion pathway (NCBI), at 3,459,045 to 3,459,482 gspG b3329: gspH - predicted general secretory pathway component, cryptic (NCBI), at 3,459,490 to 3,459,999 gspH b3330: yheH - putative export protein for general secretion pathway (GSP) (VIMSS), at 3,459,996 to 3,460,373 yheH b3331: gspJ - predicted general secretory pathway component, cryptic (NCBI), at 3,460,366 to 3,460,953 gspJ b3332: gspK - general secretory pathway component, cryptic (NCBI), at 3,460,946 to 3,461,929 gspK Position (kb) 3459 3460 3461Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 3459.001 kb on + strandat 3459.004 kb on - strandat 3459.015 kb on - strandat 3459.027 kb on - strandat 3459.078 kb on - strandat 3459.078 kb on - strandat 3459.203 kb on - strand, within gspGat 3459.203 kb on - strand, within gspGat 3459.226 kb on - strand, within gspGat 3459.244 kb on - strand, within gspGat 3459.252 kb on + strand, within gspGat 3459.252 kb on + strand, within gspGat 3459.288 kb on - strand, within gspGat 3459.288 kb on - strand, within gspGat 3459.320 kb on + strand, within gspGat 3459.320 kb on + strand, within gspGat 3459.324 kb on + strand, within gspGat 3459.355 kb on - strand, within gspGat 3459.360 kb on + strand, within gspGat 3459.360 kb on + strand, within gspGat 3459.360 kb on + strand, within gspGat 3459.360 kb on + strand, within gspGat 3459.368 kb on - strand, within gspGat 3459.371 kb on + strand, within gspGat 3459.389 kb on - strand, within gspGat 3459.391 kb on + strand, within gspGat 3459.391 kb on + strand, within gspGat 3459.391 kb on + strand, within gspGat 3459.399 kb on - strand, within gspGat 3459.421 kb on + strand, within gspGat 3459.446 kb on - strandat 3459.460 kb on - strandat 3459.460 kb on - strandat 3459.519 kb on - strandat 3459.519 kb on - strandat 3459.530 kb on - strandat 3459.530 kb on - strandat 3459.540 kb on - strandat 3459.570 kb on + strand, within gspHat 3459.578 kb on - strand, within gspHat 3459.603 kb on - strand, within gspHat 3459.607 kb on - strand, within gspHat 3459.607 kb on - strand, within gspHat 3459.674 kb on - strand, within gspHat 3459.698 kb on - strand, within gspHat 3459.716 kb on + strand, within gspHat 3459.742 kb on + strand, within gspHat 3459.742 kb on + strand, within gspHat 3459.747 kb on + strand, within gspHat 3459.818 kb on + strand, within gspHat 3459.851 kb on - strand, within gspHat 3459.851 kb on - strand, within gspHat 3459.893 kb on + strand, within gspHat 3459.920 kb on - strand, within gspHat 3459.930 kb on + strand, within gspHat 3459.937 kb on + strand, within gspHat 3459.937 kb on + strand, within gspHat 3459.947 kb on + strand, within gspHat 3459.947 kb on + strand, within gspHat 3459.955 kb on - strandat 3459.964 kb on - strandat 3459.978 kb on + strandat 3459.978 kb on + strandat 3459.978 kb on + strandat 3459.995 kb on + strandat 3460.012 kb on + strandat 3460.012 kb on + strandat 3460.031 kb on - strandat 3460.043 kb on - strand, within yheHat 3460.057 kb on + strand, within yheHat 3460.081 kb on + strand, within yheHat 3460.081 kb on + strand, within yheHat 3460.081 kb on + strand, within yheHat 3460.081 kb on + strand, within yheHat 3460.083 kb on + strand, within yheHat 3460.083 kb on + strand, within yheHat 3460.086 kb on - strand, within yheHat 3460.086 kb on - strand, within yheHat 3460.086 kb on - strand, within yheHat 3460.089 kb on - strand, within yheHat 3460.089 kb on - strand, within yheHat 3460.107 kb on + strand, within yheHat 3460.114 kb on + strand, within yheHat 3460.133 kb on + strand, within yheHat 3460.198 kb on - strand, within yheHat 3460.198 kb on - strand, within yheHat 3460.224 kb on + strand, within yheHat 3460.248 kb on - strand, within yheHat 3460.248 kb on - strand, within yheHat 3460.248 kb on - strand, within yheHat 3460.277 kb on - strand, within yheHat 3460.280 kb on + strand, within yheHat 3460.280 kb on + strand, within yheHat 3460.280 kb on + strand, within yheHat 3460.280 kb on + strand, within yheHat 3460.280 kb on + strand, within yheHat 3460.385 kb on + strandat 3460.388 kb on - strandat 3460.395 kb on + strandat 3460.395 kb on + strandat 3460.401 kb on + strandat 3460.439 kb on - strand, within gspJat 3460.518 kb on + strandat 3460.524 kb on + strand, within gspJat 3460.529 kb on - strand, within gspJat 3460.534 kb on - strand, within gspJat 3460.538 kb on - strand, within gspJat 3460.569 kb on - strand, within gspJat 3460.569 kb on - strand, within gspJat 3460.580 kb on - strand, within gspJat 3460.592 kb on - strand, within gspJat 3460.596 kb on + strand, within gspJat 3460.639 kb on - strand, within gspJat 3460.666 kb on + strandat 3460.667 kb on + strand, within gspJat 3460.668 kb on - strand, within gspJat 3460.674 kb on - strand, within gspJat 3460.686 kb on - strand, within gspJat 3460.686 kb on - strand, within gspJat 3460.705 kb on - strand, within gspJat 3460.705 kb on - strand, within gspJat 3460.707 kb on + strand, within gspJat 3460.713 kb on - strand, within gspJat 3460.713 kb on - strand, within gspJat 3460.725 kb on - strand, within gspJat 3460.725 kb on - strand, within gspJat 3460.727 kb on - strand, within gspJat 3460.732 kb on - strand, within gspJat 3460.734 kb on - strand, within gspJat 3460.770 kb on - strand, within gspJat 3460.770 kb on - strand, within gspJat 3460.770 kb on - strand, within gspJat 3460.771 kb on - strand, within gspJat 3460.835 kb on + strand, within gspJat 3460.835 kb on + strand, within gspJat 3460.848 kb on + strand, within gspJat 3460.848 kb on + strand, within gspJat 3460.854 kb on + strand, within gspJat 3460.870 kb on - strand, within gspJat 3460.872 kb on - strand, within gspJat 3460.872 kb on - strand, within gspJat 3460.877 kb on + strand, within gspJat 3460.890 kb on - strand, within gspJat 3460.900 kb on + strandat 3460.900 kb on + strandat 3460.937 kb on - strandat 3460.937 kb on - strandat 3460.978 kb on - strandat 3460.978 kb on - strandat 3460.982 kb on + strandat 3460.982 kb on + strandat 3461.004 kb on + strandat 3461.021 kb on + strandat 3461.045 kb on - strand, within gspKat 3461.045 kb on - strand, within gspKat 3461.094 kb on + strand, within gspKat 3461.129 kb on - strand, within gspKat 3461.132 kb on + strand, within gspKat 3461.139 kb on - strand, within gspKat 3461.147 kb on + strand, within gspKat 3461.176 kb on + strand, within gspKat 3461.176 kb on + strand, within gspKat 3461.176 kb on + strand, within gspKat 3461.176 kb on + strand, within gspKat 3461.178 kb on + strand, within gspKat 3461.178 kb on + strand, within gspKat 3461.184 kb on - strand, within gspKat 3461.186 kb on - strand, within gspKat 3461.186 kb on - strand, within gspKat 3461.186 kb on - strand, within gspKat 3461.201 kb on + strand, within gspKat 3461.249 kb on - strand, within gspKat 3461.253 kb on + strand, within gspKat 3461.261 kb on - strand, within gspKat 3461.289 kb on + strand, within gspKat 3461.320 kb on + strand, within gspKat 3461.323 kb on + strand, within gspKat 3461.343 kb on - strand, within gspKat 3461.343 kb on - strand, within gspKat 3461.348 kb on + strand, within gspKat 3461.348 kb on + strand, within gspKat 3461.348 kb on + strand, within gspKat 3461.352 kb on - strand, within gspK

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Per-strain Table

Position Strand Gene LocusTag Fraction Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
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3,459,001 + -0.1
3,459,004 - -0.7
3,459,015 - +0.5
3,459,027 - -0.9
3,459,078 - -1.1
3,459,078 - -2.3
3,459,203 - gspG b3328 0.36 +0.2
3,459,203 - gspG b3328 0.36 +1.4
3,459,226 - gspG b3328 0.41 -0.3
3,459,244 - gspG b3328 0.45 +0.0
3,459,252 + gspG b3328 0.47 +0.0
3,459,252 + gspG b3328 0.47 -1.2
3,459,288 - gspG b3328 0.55 -3.9
3,459,288 - gspG b3328 0.55 +0.3
3,459,320 + gspG b3328 0.63 -1.1
3,459,320 + gspG b3328 0.63 +0.1
3,459,324 + gspG b3328 0.64 -0.8
3,459,355 - gspG b3328 0.71 +2.0
3,459,360 + gspG b3328 0.72 +0.3
3,459,360 + gspG b3328 0.72 +1.7
3,459,360 + gspG b3328 0.72 -0.1
3,459,360 + gspG b3328 0.72 -0.4
3,459,368 - gspG b3328 0.74 -0.4
3,459,371 + gspG b3328 0.74 -2.3
3,459,389 - gspG b3328 0.79 -0.2
3,459,391 + gspG b3328 0.79 -0.6
3,459,391 + gspG b3328 0.79 +0.1
3,459,391 + gspG b3328 0.79 +0.9
3,459,399 - gspG b3328 0.81 -0.0
3,459,421 + gspG b3328 0.86 -1.9
3,459,446 - -4.1
3,459,460 - +1.7
3,459,460 - -3.1
3,459,519 - +0.7
3,459,519 - -1.2
3,459,530 - -1.1
3,459,530 - +0.7
3,459,540 - -1.6
3,459,570 + gspH b3329 0.16 -0.6
3,459,578 - gspH b3329 0.17 -0.4
3,459,603 - gspH b3329 0.22 -3.3
3,459,607 - gspH b3329 0.23 -0.3
3,459,607 - gspH b3329 0.23 -3.9
3,459,674 - gspH b3329 0.36 -4.2
3,459,698 - gspH b3329 0.41 -0.6
3,459,716 + gspH b3329 0.44 -2.1
3,459,742 + gspH b3329 0.49 -0.3
3,459,742 + gspH b3329 0.49 +2.1
3,459,747 + gspH b3329 0.50 +0.3
3,459,818 + gspH b3329 0.64 -0.1
3,459,851 - gspH b3329 0.71 -0.0
3,459,851 - gspH b3329 0.71 -0.2
3,459,893 + gspH b3329 0.79 -0.0
3,459,920 - gspH b3329 0.84 -1.1
3,459,930 + gspH b3329 0.86 +0.3
3,459,937 + gspH b3329 0.88 +1.2
3,459,937 + gspH b3329 0.88 +0.0
3,459,947 + gspH b3329 0.90 -2.9
3,459,947 + gspH b3329 0.90 -0.4
3,459,955 - -1.1
3,459,964 - -1.2
3,459,978 + +0.5
3,459,978 + +0.1
3,459,978 + +0.1
3,459,995 + -0.9
3,460,012 + -1.2
3,460,012 + -2.9
3,460,031 - +0.3
3,460,043 - yheH b3330 0.12 -0.6
3,460,057 + yheH b3330 0.16 -2.7
3,460,081 + yheH b3330 0.22 -0.4
3,460,081 + yheH b3330 0.22 -2.3
3,460,081 + yheH b3330 0.22 +0.3
3,460,081 + yheH b3330 0.22 +1.7
3,460,083 + yheH b3330 0.23 -2.0
3,460,083 + yheH b3330 0.23 -1.4
3,460,086 - yheH b3330 0.24 -0.0
3,460,086 - yheH b3330 0.24 -3.0
3,460,086 - yheH b3330 0.24 +0.8
3,460,089 - yheH b3330 0.25 +0.0
3,460,089 - yheH b3330 0.25 -0.0
3,460,107 + yheH b3330 0.29 -1.4
3,460,114 + yheH b3330 0.31 +0.4
3,460,133 + yheH b3330 0.36 +0.6
3,460,198 - yheH b3330 0.53 +0.0
3,460,198 - yheH b3330 0.53 -0.4
3,460,224 + yheH b3330 0.60 -2.3
3,460,248 - yheH b3330 0.67 -0.5
3,460,248 - yheH b3330 0.67 +0.5
3,460,248 - yheH b3330 0.67 -1.0
3,460,277 - yheH b3330 0.74 -0.3
3,460,280 + yheH b3330 0.75 +0.4
3,460,280 + yheH b3330 0.75 +0.7
3,460,280 + yheH b3330 0.75 -1.0
3,460,280 + yheH b3330 0.75 +0.2
3,460,280 + yheH b3330 0.75 +1.1
3,460,385 + +0.9
3,460,388 - +1.1
3,460,395 + -3.1
3,460,395 + -1.6
3,460,401 + -0.1
3,460,439 - gspJ b3331 0.12 -0.9
3,460,518 + +0.3
3,460,524 + gspJ b3331 0.27 +0.2
3,460,529 - gspJ b3331 0.28 -0.1
3,460,534 - gspJ b3331 0.29 +1.1
3,460,538 - gspJ b3331 0.29 -0.6
3,460,569 - gspJ b3331 0.35 +0.1
3,460,569 - gspJ b3331 0.35 -1.4
3,460,580 - gspJ b3331 0.36 -3.3
3,460,592 - gspJ b3331 0.38 +0.4
3,460,596 + gspJ b3331 0.39 -3.3
3,460,639 - gspJ b3331 0.46 +0.6
3,460,666 + -0.3
3,460,667 + gspJ b3331 0.51 -0.3
3,460,668 - gspJ b3331 0.51 -1.2
3,460,674 - gspJ b3331 0.52 -2.5
3,460,686 - gspJ b3331 0.54 +0.7
3,460,686 - gspJ b3331 0.54 +1.1
3,460,705 - gspJ b3331 0.58 +0.1
3,460,705 - gspJ b3331 0.58 +0.1
3,460,707 + gspJ b3331 0.58 +0.1
3,460,713 - gspJ b3331 0.59 +0.5
3,460,713 - gspJ b3331 0.59 -2.0
3,460,725 - gspJ b3331 0.61 +0.3
3,460,725 - gspJ b3331 0.61 -0.3
3,460,727 - gspJ b3331 0.61 -0.5
3,460,732 - gspJ b3331 0.62 +0.0
3,460,734 - gspJ b3331 0.63 -0.4
3,460,770 - gspJ b3331 0.69 +0.3
3,460,770 - gspJ b3331 0.69 -3.1
3,460,770 - gspJ b3331 0.69 -0.6
3,460,771 - gspJ b3331 0.69 -1.4
3,460,835 + gspJ b3331 0.80 -3.0
3,460,835 + gspJ b3331 0.80 +0.8
3,460,848 + gspJ b3331 0.82 -3.0
3,460,848 + gspJ b3331 0.82 -2.4
3,460,854 + gspJ b3331 0.83 +0.9
3,460,870 - gspJ b3331 0.86 -0.2
3,460,872 - gspJ b3331 0.86 +0.4
3,460,872 - gspJ b3331 0.86 -3.2
3,460,877 + gspJ b3331 0.87 -1.0
3,460,890 - gspJ b3331 0.89 +1.0
3,460,900 + +0.6
3,460,900 + -3.4
3,460,937 - -3.1
3,460,937 - -0.8
3,460,978 - -2.1
3,460,978 - +0.3
3,460,982 + +0.1
3,460,982 + -1.7
3,461,004 + -0.7
3,461,021 + +0.0
3,461,045 - gspK b3332 0.10 +0.6
3,461,045 - gspK b3332 0.10 -3.2
3,461,094 + gspK b3332 0.15 -1.3
3,461,129 - gspK b3332 0.19 -0.2
3,461,132 + gspK b3332 0.19 -3.8
3,461,139 - gspK b3332 0.20 +1.1
3,461,147 + gspK b3332 0.20 -0.3
3,461,176 + gspK b3332 0.23 -0.4
3,461,176 + gspK b3332 0.23 +0.7
3,461,176 + gspK b3332 0.23 +2.1
3,461,176 + gspK b3332 0.23 -3.0
3,461,178 + gspK b3332 0.24 +0.5
3,461,178 + gspK b3332 0.24 -1.3
3,461,184 - gspK b3332 0.24 -0.5
3,461,186 - gspK b3332 0.24 +0.6
3,461,186 - gspK b3332 0.24 +0.0
3,461,186 - gspK b3332 0.24 -1.4
3,461,201 + gspK b3332 0.26 -2.3
3,461,249 - gspK b3332 0.31 +0.5
3,461,253 + gspK b3332 0.31 +0.2
3,461,261 - gspK b3332 0.32 -2.4
3,461,289 + gspK b3332 0.35 +0.3
3,461,320 + gspK b3332 0.38 -0.6
3,461,323 + gspK b3332 0.38 +0.7
3,461,343 - gspK b3332 0.40 -0.1
3,461,343 - gspK b3332 0.40 -1.1
3,461,348 + gspK b3332 0.41 -0.5
3,461,348 + gspK b3332 0.41 -0.4
3,461,348 + gspK b3332 0.41 +0.4
3,461,352 - gspK b3332 0.41 -0.3

Or see this region's nucleotide sequence