Strain Fitness in Escherichia coli BW25113 around b2575

Experiment: Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnadB and yfiC overlap by 16 nucleotidesyfiC and srmB are separated by 131 nucleotides b2574: nadB - L-aspartate oxidase (NCBI), at 2,708,442 to 2,710,064 nadB b2575: yfiC - putative enzyme (VIMSS), at 2,710,049 to 2,710,786 yfiC b2576: srmB - ATP-dependent RNA helicase (NCBI), at 2,710,918 to 2,712,252 srmB Position (kb) 2710 2711Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 2709.050 kb on + strand, within nadBat 2709.135 kb on + strand, within nadBat 2709.135 kb on + strand, within nadBat 2709.188 kb on - strand, within nadBat 2709.193 kb on - strand, within nadBat 2709.377 kb on - strand, within nadBat 2709.382 kb on + strand, within nadBat 2709.496 kb on + strand, within nadBat 2709.571 kb on + strand, within nadBat 2709.578 kb on - strand, within nadBat 2709.578 kb on - strand, within nadBat 2709.579 kb on + strand, within nadBat 2709.605 kb on + strand, within nadBat 2709.623 kb on - strand, within nadBat 2709.680 kb on - strand, within nadBat 2709.709 kb on - strand, within nadBat 2709.858 kb on - strand, within nadBat 2709.900 kb on - strand, within nadBat 2709.919 kb on + strandat 2709.942 kb on - strandat 2709.942 kb on - strandat 2709.949 kb on + strandat 2709.954 kb on + strandat 2709.957 kb on - strandat 2709.997 kb on + strandat 2710.005 kb on - strandat 2710.005 kb on - strandat 2710.005 kb on - strandat 2710.189 kb on - strand, within yfiCat 2710.236 kb on + strand, within yfiCat 2710.329 kb on + strand, within yfiCat 2710.375 kb on - strand, within yfiCat 2710.398 kb on + strand, within yfiCat 2710.399 kb on - strand, within yfiCat 2710.399 kb on - strand, within yfiCat 2710.402 kb on - strand, within yfiCat 2710.409 kb on + strand, within yfiCat 2710.410 kb on + strand, within yfiCat 2710.413 kb on - strand, within yfiCat 2710.413 kb on - strand, within yfiCat 2710.418 kb on - strand, within yfiCat 2710.418 kb on - strand, within yfiCat 2710.439 kb on - strand, within yfiCat 2710.439 kb on - strand, within yfiCat 2710.591 kb on - strand, within yfiCat 2710.770 kb on + strandat 2710.770 kb on + strandat 2710.770 kb on + strandat 2710.770 kb on + strandat 2710.778 kb on - strandat 2710.778 kb on - strandat 2710.833 kb on - strandat 2710.833 kb on - strandat 2710.838 kb on + strandat 2711.086 kb on + strand, within srmBat 2711.159 kb on + strand, within srmBat 2711.181 kb on + strand, within srmBat 2711.268 kb on - strand, within srmBat 2711.305 kb on + strand, within srmBat 2711.376 kb on - strand, within srmBat 2711.425 kb on - strand, within srmBat 2711.557 kb on - strand, within srmBat 2711.666 kb on + strand, within srmBat 2711.698 kb on + strand, within srmBat 2711.747 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
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2,709,050 + nadB b2574 0.37 +3.7
2,709,135 + nadB b2574 0.43 -0.0
2,709,135 + nadB b2574 0.43 -1.4
2,709,188 - nadB b2574 0.46 +0.7
2,709,193 - nadB b2574 0.46 +0.3
2,709,377 - nadB b2574 0.58 -2.7
2,709,382 + nadB b2574 0.58 -2.5
2,709,496 + nadB b2574 0.65 +1.3
2,709,571 + nadB b2574 0.70 -0.4
2,709,578 - nadB b2574 0.70 -3.4
2,709,578 - nadB b2574 0.70 -0.2
2,709,579 + nadB b2574 0.70 +0.6
2,709,605 + nadB b2574 0.72 -0.9
2,709,623 - nadB b2574 0.73 -1.5
2,709,680 - nadB b2574 0.76 +0.5
2,709,709 - nadB b2574 0.78 -0.1
2,709,858 - nadB b2574 0.87 -1.4
2,709,900 - nadB b2574 0.90 -2.5
2,709,919 + -2.1
2,709,942 - -1.8
2,709,942 - -1.0
2,709,949 + +0.2
2,709,954 + -1.8
2,709,957 - -2.7
2,709,997 + +0.9
2,710,005 - +1.1
2,710,005 - -0.6
2,710,005 - +1.2
2,710,189 - yfiC b2575 0.19 -1.2
2,710,236 + yfiC b2575 0.25 -0.1
2,710,329 + yfiC b2575 0.38 -5.1
2,710,375 - yfiC b2575 0.44 -0.0
2,710,398 + yfiC b2575 0.47 -0.2
2,710,399 - yfiC b2575 0.47 +0.2
2,710,399 - yfiC b2575 0.47 +0.3
2,710,402 - yfiC b2575 0.48 -1.0
2,710,409 + yfiC b2575 0.49 -0.9
2,710,410 + yfiC b2575 0.49 -1.3
2,710,413 - yfiC b2575 0.49 -2.2
2,710,413 - yfiC b2575 0.49 -4.4
2,710,418 - yfiC b2575 0.50 -0.3
2,710,418 - yfiC b2575 0.50 +0.2
2,710,439 - yfiC b2575 0.53 +1.5
2,710,439 - yfiC b2575 0.53 -0.2
2,710,591 - yfiC b2575 0.73 -0.2
2,710,770 + -0.8
2,710,770 + +0.6
2,710,770 + +0.6
2,710,770 + +0.2
2,710,778 - -3.0
2,710,778 - -3.1
2,710,833 - +1.0
2,710,833 - -0.2
2,710,838 + -0.9
2,711,086 + srmB b2576 0.13 -0.8
2,711,159 + srmB b2576 0.18 -0.8
2,711,181 + srmB b2576 0.20 -1.0
2,711,268 - srmB b2576 0.26 -0.3
2,711,305 + srmB b2576 0.29 -1.1
2,711,376 - srmB b2576 0.34 -1.4
2,711,425 - srmB b2576 0.38 -0.5
2,711,557 - srmB b2576 0.48 -1.7
2,711,666 + srmB b2576 0.56 -1.1
2,711,698 + srmB b2576 0.58 +0.0
2,711,747 + +1.0

Or see this region's nucleotide sequence