Experiment: Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt appA and yccC are separated by 114 nucleotides yccC and yccY are separated by 19 nucleotides yccY and yccZ overlap by 13 nucleotides
b0980: appA - phosphoanhydride phosphorylase (NCBI), at 1,039,840 to 1,041,138
appA
b0981: yccC - cryptic autophosphorylating protein tyrosine kinase Etk (NCBI), at 1,041,253 to 1,043,433
yccC
b0982: yccY - putative phosphatase (VIMSS), at 1,043,453 to 1,043,899
yccY
b0983: yccZ - predicted exopolysaccharide export protein (NCBI), at 1,043,887 to 1,045,026
yccZ
Position (kb)
1041
1042
1043
1044 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1040.254 kb on + strand, within appA at 1040.348 kb on - strand, within appA at 1040.782 kb on - strand, within appA at 1040.797 kb on - strand, within appA at 1040.816 kb on + strand, within appA at 1040.816 kb on + strand, within appA at 1040.822 kb on - strand, within appA at 1040.822 kb on - strand, within appA at 1040.874 kb on + strand, within appA at 1040.894 kb on + strand, within appA at 1040.894 kb on + strand, within appA at 1041.148 kb on - strand at 1041.148 kb on - strand at 1041.171 kb on + strand at 1041.197 kb on - strand at 1041.197 kb on - strand at 1041.272 kb on + strand at 1041.286 kb on - strand at 1041.331 kb on + strand at 1041.331 kb on + strand at 1041.339 kb on - strand at 1041.360 kb on - strand at 1041.366 kb on + strand at 1041.419 kb on - strand at 1041.554 kb on + strand, within yccC at 1041.554 kb on + strand, within yccC at 1041.573 kb on + strand, within yccC at 1041.573 kb on + strand, within yccC at 1041.695 kb on + strand, within yccC at 1042.035 kb on - strand, within yccC at 1042.035 kb on - strand, within yccC at 1042.053 kb on + strand, within yccC at 1042.180 kb on + strand, within yccC at 1042.180 kb on + strand, within yccC at 1042.202 kb on - strand, within yccC at 1042.202 kb on - strand, within yccC at 1042.283 kb on + strand, within yccC at 1042.582 kb on + strand, within yccC at 1042.582 kb on + strand, within yccC at 1042.607 kb on - strand, within yccC at 1042.704 kb on + strand, within yccC at 1042.704 kb on + strand, within yccC at 1042.988 kb on + strand, within yccC at 1043.081 kb on + strand, within yccC at 1043.081 kb on + strand, within yccC at 1043.099 kb on + strand, within yccC at 1043.278 kb on + strand at 1043.278 kb on + strand at 1043.345 kb on - strand at 1043.345 kb on - strand at 1043.391 kb on + strand at 1043.500 kb on - strand, within yccY at 1043.530 kb on - strand, within yccY at 1043.635 kb on - strand, within yccY at 1043.686 kb on + strand, within yccY at 1043.710 kb on + strand, within yccY at 1043.710 kb on + strand, within yccY at 1043.812 kb on - strand, within yccY at 1043.829 kb on - strand, within yccY at 1043.918 kb on + strand at 1043.918 kb on + strand at 1043.929 kb on + strand at 1043.929 kb on + strand at 1043.958 kb on + strand at 1044.203 kb on - strand, within yccZ at 1044.397 kb on - strand, within yccZ at 1044.397 kb on - strand, within yccZ
Per-strain Table
Position Strand Gene LocusTag Fraction Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB remove 1,040,254 + appA b0980 0.32 -3.8 1,040,348 - appA b0980 0.39 +0.0 1,040,782 - appA b0980 0.73 -0.3 1,040,797 - appA b0980 0.74 +0.3 1,040,816 + appA b0980 0.75 -1.0 1,040,816 + appA b0980 0.75 +1.2 1,040,822 - appA b0980 0.76 -1.0 1,040,822 - appA b0980 0.76 -0.9 1,040,874 + appA b0980 0.80 +0.6 1,040,894 + appA b0980 0.81 -2.4 1,040,894 + appA b0980 0.81 -3.0 1,041,148 - -0.3 1,041,148 - -0.1 1,041,171 + +0.4 1,041,197 - -3.7 1,041,197 - +1.5 1,041,272 + -0.2 1,041,286 - -2.4 1,041,331 + +1.3 1,041,331 + +1.1 1,041,339 - +0.4 1,041,360 - -0.4 1,041,366 + +0.5 1,041,419 - -1.0 1,041,554 + yccC b0981 0.14 -1.6 1,041,554 + yccC b0981 0.14 +0.9 1,041,573 + yccC b0981 0.15 +1.4 1,041,573 + yccC b0981 0.15 -0.5 1,041,695 + yccC b0981 0.20 +1.0 1,042,035 - yccC b0981 0.36 -2.1 1,042,035 - yccC b0981 0.36 +0.8 1,042,053 + yccC b0981 0.37 +2.6 1,042,180 + yccC b0981 0.43 +1.3 1,042,180 + yccC b0981 0.43 +0.4 1,042,202 - yccC b0981 0.44 -3.8 1,042,202 - yccC b0981 0.44 +0.8 1,042,283 + yccC b0981 0.47 +0.5 1,042,582 + yccC b0981 0.61 -2.9 1,042,582 + yccC b0981 0.61 +0.2 1,042,607 - yccC b0981 0.62 +0.3 1,042,704 + yccC b0981 0.67 +1.3 1,042,704 + yccC b0981 0.67 +1.2 1,042,988 + yccC b0981 0.80 -0.4 1,043,081 + yccC b0981 0.84 +1.4 1,043,081 + yccC b0981 0.84 +0.5 1,043,099 + yccC b0981 0.85 -0.2 1,043,278 + -3.5 1,043,278 + +0.1 1,043,345 - -0.5 1,043,345 - +0.4 1,043,391 + +0.7 1,043,500 - yccY b0982 0.11 +1.5 1,043,530 - yccY b0982 0.17 +0.2 1,043,635 - yccY b0982 0.41 +0.2 1,043,686 + yccY b0982 0.52 -0.3 1,043,710 + yccY b0982 0.57 +0.6 1,043,710 + yccY b0982 0.57 +1.1 1,043,812 - yccY b0982 0.80 +1.2 1,043,829 - yccY b0982 0.84 -0.2 1,043,918 + +1.2 1,043,918 + -1.9 1,043,929 + -2.2 1,043,929 + +0.2 1,043,958 + -1.0 1,044,203 - yccZ b0983 0.28 -1.6 1,044,397 - yccZ b0983 0.45 +0.3 1,044,397 - yccZ b0983 0.45 +0.4
Or see this region's nucleotide sequence