Experiment: Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ykgE and ykgF are separated by 10 nucleotides ykgF and ykgG overlap by 8 nucleotides ykgG and ykgH are separated by 242 nucleotides
b0306: ykgE - predicted oxidoreductase (NCBI), at 320,832 to 321,551
ykgE
b0307: ykgF - predicted amino acid dehydrogenase with NAD(P)-binding domain and ferridoxin-like domain (NCBI), at 321,562 to 322,989
ykgF
b0308: ykgG - putative transporter (VIMSS), at 322,982 to 323,677
ykgG
b0310: ykgH - predicted inner membrane protein (NCBI), at 323,920 to 324,588
ykgH
Position (kb) 321
322
323 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 320.576 kb on - strand at 320.654 kb on - strand at 320.723 kb on + strand at 320.728 kb on + strand at 320.729 kb on + strand at 320.729 kb on - strand at 320.731 kb on - strand at 320.781 kb on - strand at 320.788 kb on - strand at 320.788 kb on - strand at 320.807 kb on + strand at 320.813 kb on + strand at 320.815 kb on - strand at 320.821 kb on - strand at 320.865 kb on - strand at 320.883 kb on - strand at 320.884 kb on - strand at 320.886 kb on + strand at 320.886 kb on + strand at 320.894 kb on + strand at 320.894 kb on + strand at 320.898 kb on + strand at 320.898 kb on + strand at 320.899 kb on - strand at 320.901 kb on - strand at 320.904 kb on - strand, within ykgE at 320.944 kb on + strand, within ykgE at 320.944 kb on + strand, within ykgE at 320.959 kb on + strand, within ykgE at 320.959 kb on + strand, within ykgE at 320.971 kb on - strand, within ykgE at 320.975 kb on - strand, within ykgE at 320.975 kb on - strand, within ykgE at 320.977 kb on - strand, within ykgE at 320.978 kb on - strand, within ykgE at 320.983 kb on + strand, within ykgE at 321.022 kb on + strand, within ykgE at 321.027 kb on + strand, within ykgE at 321.027 kb on + strand, within ykgE at 321.035 kb on - strand, within ykgE at 321.065 kb on + strand, within ykgE at 321.073 kb on - strand, within ykgE at 321.106 kb on - strand, within ykgE at 321.202 kb on - strand, within ykgE at 321.221 kb on - strand, within ykgE at 321.221 kb on - strand, within ykgE at 321.236 kb on - strand, within ykgE at 321.236 kb on - strand, within ykgE at 321.249 kb on - strand, within ykgE at 321.266 kb on + strand, within ykgE at 321.307 kb on + strand, within ykgE at 321.321 kb on + strand, within ykgE at 321.325 kb on + strand, within ykgE at 321.325 kb on + strand, within ykgE at 321.335 kb on + strand, within ykgE at 321.436 kb on - strand, within ykgE at 321.461 kb on + strand, within ykgE at 321.461 kb on + strand, within ykgE at 321.533 kb on + strand at 321.533 kb on + strand at 321.552 kb on + strand at 321.553 kb on + strand at 321.557 kb on + strand at 321.672 kb on - strand at 321.699 kb on + strand at 321.732 kb on + strand, within ykgF at 321.732 kb on + strand, within ykgF at 321.736 kb on + strand, within ykgF at 321.750 kb on + strand, within ykgF at 321.918 kb on + strand, within ykgF at 321.960 kb on + strand, within ykgF at 321.960 kb on + strand, within ykgF at 322.013 kb on + strand, within ykgF at 322.028 kb on + strand, within ykgF at 322.028 kb on + strand, within ykgF at 322.034 kb on + strand, within ykgF at 322.075 kb on + strand, within ykgF at 322.081 kb on + strand, within ykgF at 322.154 kb on + strand, within ykgF at 322.239 kb on + strand, within ykgF at 322.477 kb on - strand, within ykgF at 322.516 kb on + strand, within ykgF at 322.516 kb on + strand, within ykgF at 322.560 kb on - strand, within ykgF at 322.641 kb on + strand, within ykgF at 322.660 kb on + strand, within ykgF at 323.074 kb on + strand, within ykgG at 323.074 kb on + strand, within ykgG at 323.094 kb on + strand, within ykgG at 323.105 kb on + strand, within ykgG at 323.168 kb on + strand, within ykgG at 323.237 kb on + strand, within ykgG at 323.307 kb on - strand, within ykgG at 323.346 kb on - strand, within ykgG at 323.346 kb on - strand, within ykgG at 323.359 kb on + strand, within ykgG at 323.359 kb on + strand, within ykgG at 323.500 kb on + strand, within ykgG at 323.503 kb on + strand, within ykgG at 323.503 kb on - strand, within ykgG at 323.583 kb on - strand, within ykgG at 323.612 kb on - strand at 323.614 kb on + strand at 323.614 kb on + strand at 323.621 kb on - strand at 323.623 kb on + strand at 323.623 kb on + strand at 323.623 kb on + strand at 323.628 kb on + strand at 323.628 kb on + strand at 323.630 kb on + strand at 323.630 kb on + strand at 323.630 kb on + strand at 323.630 kb on + strand at 323.630 kb on + strand at 323.630 kb on + strand at 323.630 kb on + strand at 323.651 kb on + strand at 323.690 kb on + strand at 323.696 kb on + strand at 323.698 kb on - strand at 323.701 kb on + strand at 323.701 kb on + strand at 323.712 kb on + strand at 323.712 kb on + strand at 323.712 kb on + strand at 323.712 kb on + strand at 323.742 kb on + strand at 323.748 kb on + strand at 323.748 kb on + strand at 323.748 kb on + strand at 323.749 kb on + strand at 323.757 kb on + strand at 323.757 kb on + strand at 323.759 kb on + strand at 323.759 kb on + strand at 323.759 kb on + strand at 323.874 kb on + strand at 323.874 kb on + strand at 323.889 kb on + strand at 323.892 kb on - strand at 323.892 kb on - strand at 323.892 kb on - strand at 323.892 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB remove 320,576 - +2.1 320,654 - -0.4 320,723 + -0.9 320,728 + -0.2 320,729 + +0.3 320,729 - +2.1 320,731 - +2.1 320,781 - -1.9 320,788 - +1.4 320,788 - +3.6 320,807 + -0.7 320,813 + +0.8 320,815 - +1.8 320,821 - +0.6 320,865 - +0.8 320,883 - +0.8 320,884 - -0.3 320,886 + -1.4 320,886 + +0.5 320,894 + +0.2 320,894 + -3.4 320,898 + +2.0 320,898 + +0.5 320,899 - +1.2 320,901 - -0.4 320,904 - ykgE b0306 0.10 +0.0 320,944 + ykgE b0306 0.16 -1.1 320,944 + ykgE b0306 0.16 +1.6 320,959 + ykgE b0306 0.18 -1.4 320,959 + ykgE b0306 0.18 -0.8 320,971 - ykgE b0306 0.19 -1.3 320,975 - ykgE b0306 0.20 -0.9 320,975 - ykgE b0306 0.20 +0.8 320,977 - ykgE b0306 0.20 +0.9 320,978 - ykgE b0306 0.20 -1.9 320,983 + ykgE b0306 0.21 +0.5 321,022 + ykgE b0306 0.26 +0.6 321,027 + ykgE b0306 0.27 -3.3 321,027 + ykgE b0306 0.27 -0.3 321,035 - ykgE b0306 0.28 -0.1 321,065 + ykgE b0306 0.32 +1.0 321,073 - ykgE b0306 0.33 +0.3 321,106 - ykgE b0306 0.38 +0.3 321,202 - ykgE b0306 0.51 -0.5 321,221 - ykgE b0306 0.54 +1.8 321,221 - ykgE b0306 0.54 +1.2 321,236 - ykgE b0306 0.56 +0.2 321,236 - ykgE b0306 0.56 -1.3 321,249 - ykgE b0306 0.58 +0.2 321,266 + ykgE b0306 0.60 -0.0 321,307 + ykgE b0306 0.66 -0.1 321,321 + ykgE b0306 0.68 -0.3 321,325 + ykgE b0306 0.68 -0.7 321,325 + ykgE b0306 0.68 -0.7 321,335 + ykgE b0306 0.70 -0.8 321,436 - ykgE b0306 0.84 +1.0 321,461 + ykgE b0306 0.87 -3.3 321,461 + ykgE b0306 0.87 +0.4 321,533 + +0.9 321,533 + +0.9 321,552 + +0.7 321,553 + +1.3 321,557 + -0.9 321,672 - -5.4 321,699 + +2.5 321,732 + ykgF b0307 0.12 +1.8 321,732 + ykgF b0307 0.12 +1.7 321,736 + ykgF b0307 0.12 -0.4 321,750 + ykgF b0307 0.13 +0.0 321,918 + ykgF b0307 0.25 -1.3 321,960 + ykgF b0307 0.28 -3.3 321,960 + ykgF b0307 0.28 -3.5 322,013 + ykgF b0307 0.32 +1.7 322,028 + ykgF b0307 0.33 +2.8 322,028 + ykgF b0307 0.33 +0.1 322,034 + ykgF b0307 0.33 +0.4 322,075 + ykgF b0307 0.36 -3.4 322,081 + ykgF b0307 0.36 +0.6 322,154 + ykgF b0307 0.41 +0.6 322,239 + ykgF b0307 0.47 +1.1 322,477 - ykgF b0307 0.64 -2.5 322,516 + ykgF b0307 0.67 -3.2 322,516 + ykgF b0307 0.67 +1.2 322,560 - ykgF b0307 0.70 -1.9 322,641 + ykgF b0307 0.76 +1.1 322,660 + ykgF b0307 0.77 +1.8 323,074 + ykgG b0308 0.13 -1.0 323,074 + ykgG b0308 0.13 -1.6 323,094 + ykgG b0308 0.16 -1.2 323,105 + ykgG b0308 0.18 +0.8 323,168 + ykgG b0308 0.27 +0.2 323,237 + ykgG b0308 0.37 -0.3 323,307 - ykgG b0308 0.47 +2.1 323,346 - ykgG b0308 0.52 +2.1 323,346 - ykgG b0308 0.52 +0.1 323,359 + ykgG b0308 0.54 -0.9 323,359 + ykgG b0308 0.54 -4.6 323,500 + ykgG b0308 0.74 -0.1 323,503 + ykgG b0308 0.75 -1.0 323,503 - ykgG b0308 0.75 +0.5 323,583 - ykgG b0308 0.86 -3.0 323,612 - +0.5 323,614 + +0.6 323,614 + +0.2 323,621 - +1.1 323,623 + +0.5 323,623 + +1.3 323,623 + +2.5 323,628 + -0.7 323,628 + -2.1 323,630 + -0.3 323,630 + +1.1 323,630 + +1.2 323,630 + +1.1 323,630 + +0.2 323,630 + -4.9 323,630 + +0.4 323,651 + -0.4 323,690 + +1.0 323,696 + -0.8 323,698 - +2.1 323,701 + -0.5 323,701 + -1.7 323,712 + -0.1 323,712 + +0.4 323,712 + +0.1 323,712 + +1.4 323,742 + -0.1 323,748 + +0.1 323,748 + +2.0 323,748 + +1.2 323,749 + -2.1 323,757 + +1.2 323,757 + -4.1 323,759 + -1.9 323,759 + -2.5 323,759 + +0.4 323,874 + +2.0 323,874 + +2.0 323,889 + +2.0 323,892 - +2.0 323,892 - +2.0 323,892 - +2.0 323,892 - +2.0
Or see this region's nucleotide sequence