Experiment: Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yagR and yagS overlap by 4 nucleotides yagS and yagT overlap by 4 nucleotides yagT and yagU are separated by 417 nucleotides
b0284: yagR - predicted oxidoreductase with molybdenum-binding domain (NCBI), at 297,960 to 300,158
yagR
b0285: yagS - predicted oxidoreductase with FAD-binding domain (NCBI), at 300,155 to 301,111
yagS
b0286: yagT - predicted xanthine dehydrogenase, 2Fe-2S subunit (NCBI), at 301,108 to 301,797
yagT
b0287: yagU - conserved inner membrane protein (NCBI), at 302,215 to 302,829
yagU
Position (kb) 301
302 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 300.109 kb on + strand at 300.109 kb on + strand at 300.176 kb on + strand at 300.181 kb on + strand at 300.181 kb on + strand at 300.196 kb on - strand at 300.268 kb on - strand, within yagS at 300.268 kb on - strand, within yagS at 300.325 kb on + strand, within yagS at 300.385 kb on + strand, within yagS at 300.483 kb on + strand, within yagS at 300.486 kb on - strand, within yagS at 300.486 kb on - strand, within yagS at 300.544 kb on + strand, within yagS at 300.552 kb on - strand, within yagS at 300.581 kb on + strand, within yagS at 300.719 kb on + strand, within yagS at 300.798 kb on + strand, within yagS at 300.829 kb on - strand, within yagS at 300.887 kb on - strand, within yagS at 300.916 kb on - strand, within yagS at 301.099 kb on - strand at 301.177 kb on + strand, within yagT at 301.287 kb on + strand, within yagT at 301.300 kb on + strand, within yagT at 301.300 kb on + strand, within yagT at 301.344 kb on - strand, within yagT at 301.428 kb on - strand, within yagT at 301.483 kb on - strand, within yagT at 301.483 kb on - strand, within yagT at 301.489 kb on - strand, within yagT at 301.580 kb on - strand, within yagT at 301.580 kb on - strand, within yagT at 301.638 kb on - strand, within yagT at 301.638 kb on - strand, within yagT at 301.672 kb on - strand, within yagT at 301.790 kb on - strand at 301.795 kb on + strand at 301.795 kb on + strand at 301.797 kb on + strand at 301.797 kb on + strand at 301.797 kb on + strand at 301.800 kb on - strand at 301.833 kb on - strand at 301.857 kb on - strand at 301.955 kb on + strand at 301.963 kb on - strand at 301.986 kb on + strand at 301.996 kb on - strand at 302.030 kb on + strand at 302.030 kb on + strand at 302.030 kb on + strand at 302.030 kb on + strand at 302.030 kb on + strand at 302.030 kb on + strand at 302.030 kb on + strand at 302.030 kb on + strand at 302.030 kb on + strand at 302.030 kb on + strand at 302.030 kb on + strand at 302.031 kb on + strand at 302.033 kb on + strand at 302.033 kb on + strand at 302.033 kb on + strand at 302.038 kb on - strand at 302.038 kb on - strand at 302.038 kb on - strand at 302.038 kb on - strand at 302.038 kb on - strand at 302.038 kb on - strand at 302.038 kb on - strand at 302.038 kb on - strand at 302.038 kb on - strand at 302.040 kb on + strand at 302.048 kb on - strand at 302.048 kb on - strand at 302.145 kb on + strand at 302.175 kb on + strand at 302.182 kb on + strand at 302.183 kb on - strand at 302.185 kb on + strand at 302.198 kb on - strand at 302.204 kb on - strand at 302.205 kb on - strand at 302.207 kb on + strand at 302.212 kb on + strand at 302.212 kb on + strand at 302.215 kb on - strand at 302.220 kb on - strand at 302.220 kb on - strand at 302.220 kb on - strand at 302.242 kb on + strand at 302.254 kb on - strand at 302.254 kb on - strand at 302.258 kb on + strand at 302.261 kb on - strand at 302.261 kb on - strand at 302.261 kb on - strand at 302.261 kb on - strand at 302.271 kb on + strand at 302.274 kb on - strand at 302.274 kb on - strand at 302.278 kb on - strand, within yagU at 302.293 kb on + strand, within yagU at 302.302 kb on + strand, within yagU at 302.304 kb on + strand, within yagU at 302.308 kb on + strand, within yagU at 302.316 kb on - strand, within yagU at 302.318 kb on - strand, within yagU at 302.340 kb on + strand, within yagU at 302.355 kb on + strand, within yagU at 302.355 kb on + strand, within yagU at 302.367 kb on + strand, within yagU at 302.377 kb on + strand, within yagU at 302.380 kb on + strand, within yagU at 302.380 kb on + strand, within yagU at 302.387 kb on - strand, within yagU at 302.403 kb on + strand, within yagU at 302.442 kb on - strand, within yagU at 302.457 kb on + strand, within yagU at 302.464 kb on - strand, within yagU at 302.470 kb on - strand, within yagU at 302.485 kb on + strand, within yagU at 302.485 kb on + strand, within yagU at 302.485 kb on + strand, within yagU at 302.495 kb on - strand, within yagU at 302.506 kb on + strand, within yagU at 302.508 kb on + strand, within yagU at 302.514 kb on - strand, within yagU at 302.514 kb on - strand, within yagU at 302.516 kb on + strand, within yagU at 302.516 kb on + strand, within yagU at 302.517 kb on + strand, within yagU at 302.517 kb on + strand, within yagU at 302.524 kb on + strand, within yagU at 302.524 kb on + strand, within yagU at 302.525 kb on - strand, within yagU at 302.530 kb on + strand, within yagU at 302.538 kb on - strand, within yagU at 302.561 kb on + strand, within yagU at 302.608 kb on + strand, within yagU at 302.608 kb on + strand, within yagU at 302.608 kb on + strand, within yagU at 302.616 kb on - strand, within yagU at 302.616 kb on - strand, within yagU at 302.623 kb on + strand, within yagU at 302.626 kb on - strand, within yagU at 302.656 kb on + strand, within yagU at 302.663 kb on + strand, within yagU at 302.664 kb on + strand, within yagU at 302.664 kb on + strand, within yagU at 302.668 kb on + strand, within yagU at 302.668 kb on + strand, within yagU at 302.668 kb on + strand, within yagU at 302.668 kb on + strand, within yagU at 302.675 kb on + strand, within yagU at 302.675 kb on + strand, within yagU at 302.675 kb on + strand, within yagU at 302.675 kb on + strand, within yagU at 302.675 kb on + strand, within yagU at 302.676 kb on - strand, within yagU at 302.690 kb on + strand, within yagU at 302.694 kb on + strand, within yagU at 302.780 kb on + strand at 302.780 kb on + strand at 302.782 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB remove 300,109 + -3.7 300,109 + +0.1 300,176 + +1.2 300,181 + -0.4 300,181 + +0.1 300,196 - +0.1 300,268 - yagS b0285 0.12 -1.5 300,268 - yagS b0285 0.12 -0.2 300,325 + yagS b0285 0.18 +0.7 300,385 + yagS b0285 0.24 +1.2 300,483 + yagS b0285 0.34 -1.7 300,486 - yagS b0285 0.35 +0.2 300,486 - yagS b0285 0.35 -3.9 300,544 + yagS b0285 0.41 -0.4 300,552 - yagS b0285 0.41 +0.4 300,581 + yagS b0285 0.45 +0.5 300,719 + yagS b0285 0.59 +0.6 300,798 + yagS b0285 0.67 -0.3 300,829 - yagS b0285 0.70 +1.0 300,887 - yagS b0285 0.76 -4.4 300,916 - yagS b0285 0.80 +0.7 301,099 - +1.1 301,177 + yagT b0286 0.10 +0.1 301,287 + yagT b0286 0.26 -3.6 301,300 + yagT b0286 0.28 -1.9 301,300 + yagT b0286 0.28 -0.2 301,344 - yagT b0286 0.34 -0.9 301,428 - yagT b0286 0.46 -0.2 301,483 - yagT b0286 0.54 +0.7 301,483 - yagT b0286 0.54 +0.2 301,489 - yagT b0286 0.55 -2.1 301,580 - yagT b0286 0.68 -1.1 301,580 - yagT b0286 0.68 -0.5 301,638 - yagT b0286 0.77 -2.2 301,638 - yagT b0286 0.77 +0.3 301,672 - yagT b0286 0.82 +0.2 301,790 - -1.2 301,795 + +0.2 301,795 + -0.7 301,797 + +1.3 301,797 + +0.4 301,797 + -2.6 301,800 - +1.0 301,833 - +1.2 301,857 - +0.0 301,955 + -0.8 301,963 - +2.1 301,986 + +1.9 301,996 - -1.4 302,030 + +0.7 302,030 + -1.2 302,030 + -1.6 302,030 + +0.2 302,030 + -0.9 302,030 + -2.6 302,030 + -2.2 302,030 + +0.1 302,030 + -2.3 302,030 + -0.1 302,030 + +1.0 302,031 + -0.8 302,033 + +0.1 302,033 + -1.4 302,033 + -4.1 302,038 - +0.1 302,038 - +2.1 302,038 - +4.3 302,038 - +1.8 302,038 - +2.1 302,038 - +0.7 302,038 - +2.1 302,038 - +2.1 302,038 - -0.2 302,040 + +0.2 302,048 - +3.4 302,048 - -0.5 302,145 + +0.4 302,175 + +0.8 302,182 + -3.9 302,183 - -0.5 302,185 + +1.8 302,198 - -3.8 302,204 - -4.6 302,205 - +1.9 302,207 + +1.1 302,212 + +0.6 302,212 + -0.8 302,215 - -0.2 302,220 - +0.2 302,220 - +0.5 302,220 - +1.1 302,242 + +1.8 302,254 - +1.6 302,254 - -0.9 302,258 + -1.0 302,261 - -2.9 302,261 - +1.1 302,261 - +0.5 302,261 - -0.7 302,271 + -2.8 302,274 - -1.6 302,274 - +1.1 302,278 - yagU b0287 0.10 +1.0 302,293 + yagU b0287 0.13 -2.2 302,302 + yagU b0287 0.14 +1.6 302,304 + yagU b0287 0.14 +0.3 302,308 + yagU b0287 0.15 -2.8 302,316 - yagU b0287 0.16 -0.2 302,318 - yagU b0287 0.17 +1.1 302,340 + yagU b0287 0.20 -5.1 302,355 + yagU b0287 0.23 -2.2 302,355 + yagU b0287 0.23 -1.6 302,367 + yagU b0287 0.25 +0.0 302,377 + yagU b0287 0.26 -2.4 302,380 + yagU b0287 0.27 -0.7 302,380 + yagU b0287 0.27 -1.1 302,387 - yagU b0287 0.28 +1.1 302,403 + yagU b0287 0.31 +1.4 302,442 - yagU b0287 0.37 -0.8 302,457 + yagU b0287 0.39 -1.7 302,464 - yagU b0287 0.40 +0.5 302,470 - yagU b0287 0.41 -0.5 302,485 + yagU b0287 0.44 +0.6 302,485 + yagU b0287 0.44 -1.1 302,485 + yagU b0287 0.44 +0.3 302,495 - yagU b0287 0.46 +2.1 302,506 + yagU b0287 0.47 +0.8 302,508 + yagU b0287 0.48 +2.0 302,514 - yagU b0287 0.49 -0.5 302,514 - yagU b0287 0.49 +2.1 302,516 + yagU b0287 0.49 +0.3 302,516 + yagU b0287 0.49 -0.4 302,517 + yagU b0287 0.49 +0.8 302,517 + yagU b0287 0.49 -1.1 302,524 + yagU b0287 0.50 +1.3 302,524 + yagU b0287 0.50 -0.0 302,525 - yagU b0287 0.50 -0.5 302,530 + yagU b0287 0.51 -3.7 302,538 - yagU b0287 0.53 +1.1 302,561 + yagU b0287 0.56 +1.0 302,608 + yagU b0287 0.64 -0.7 302,608 + yagU b0287 0.64 +0.1 302,608 + yagU b0287 0.64 +1.1 302,616 - yagU b0287 0.65 +2.1 302,616 - yagU b0287 0.65 +2.1 302,623 + yagU b0287 0.66 -3.2 302,626 - yagU b0287 0.67 +2.1 302,656 + yagU b0287 0.72 -2.1 302,663 + yagU b0287 0.73 +0.4 302,664 + yagU b0287 0.73 -1.7 302,664 + yagU b0287 0.73 -2.2 302,668 + yagU b0287 0.74 +0.9 302,668 + yagU b0287 0.74 +0.5 302,668 + yagU b0287 0.74 +0.1 302,668 + yagU b0287 0.74 -0.6 302,675 + yagU b0287 0.75 -0.5 302,675 + yagU b0287 0.75 -1.7 302,675 + yagU b0287 0.75 -5.3 302,675 + yagU b0287 0.75 -4.0 302,675 + yagU b0287 0.75 +0.3 302,676 - yagU b0287 0.75 +2.1 302,690 + yagU b0287 0.77 -0.5 302,694 + yagU b0287 0.78 +0.4 302,780 + -3.3 302,780 + +2.2 302,782 + -1.4
Or see this region's nucleotide sequence